CNGA4 (NM_001037329) Human Tagged ORF Clone

CAT#: RC218174

CNGA4 (Myc-DDK-tagged)-Human cyclic nucleotide gated channel alpha 4 (CNGA4)

Product Images

Circular map for RC218174

• 

  Western blot validation of overexpression lysate (Cat# LY421949) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218174 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNGA4
• Synonyms: CNCA2; CNG-4; CNG4; CNG5; CNGB2; OCNC2; OCNCb; OCNCBETA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218174 representing NM_001037329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGGACACCAAAGTGAAGACAACAGAGTCCAGTCCCCCAGCCCCATCCAAGGCCAGGAAGTTGC
  TGCCTGTCCTGGACCCATCTGGGGATTACTACTACTGGTGGCTGAACACAATGGTCTTCCCAGTCATGTA
  TAACCTCATCATCCTCGTGTGCAGAGCCTGCTTCCCCGACTTGCAGCACGGTTATCTGGTGGCCTGGTTG
  GTGCTGGACTACACGAGTGACCTGCTATACCTACTAGACATGGTGGTGCGCTTCCACACAGGATTCTTGG
  AACAGGGCATCCTGGTGGTGGACAAGGGTAGGATCTCGAGTCGCTACGTTCGCACCTGGAGTTTCTTCTT
  GGACCTGGCTTCCCTGATGCCCACAGATGTGGTCTACGTGCGGCTGGGCCCGCACACACCCACCCTGAGG
  CTGAACCGCTTTCTCCGCGCGCCCCGCCTCTTCGAGGCCTTCGACCGCACAGAGACCCGCACAGCTTACC
  CAAATGCCTTTCGCATTGCCAAGCTGATGCTTTACATTTTTGTCGTCATCCATTGGAACAGCTGCCTATA
  CTTTGCCCTATCCCGGTACCTGGGCTTCGGGCGTGACGCATGGGTGTACCCGGACCCCGCGCAGCCTGGC
  TTTGAGCGCCTGCGGCGCCAGTACCTCTATAGCTTTTACTTCTCCACGCTGATACTGACTACAGTGGGCG
  ATACACCGCCGCCAGCCAGGGAAGAAGAGTACCTCTTCATGGTGGGCGACTTCCTGCTGGCCGTCATGGG
  TTTCGCCACCATCATGGGTAGCATGAGCTCTGTCATCTACAACATGAACACTGCAGATGCGGCTTTCTAC
  CCAGATCATGCACTGGTGAAGAAGTACATGAAGCTGCAGCACGTCAACCGCAAGCTGGAGCGGCGAGTTA
  TTGACTGGTATCAGCACCTGCAGATCAACAAGAAGATGACCAACGAGGTAGCCATCTTACAGCACTTGCC
  TGAGCGGCTGCGGGCAGAAGTGGCTGTGTCTGTGCACCTGTCCACTCTGAGCCGGGTGCAGATCTTTCAG
  AACTGTGAGGCCAGCCTGCTGGAGGAGCTGGTGCTGAAGCTGCAGCCCCAGACCTACTCACCAGGTGAAT
  ATGTATGCCGCAAAGGAGACATTGGCCAAGAGATGTACATCATCCGAGAGGGTCAACTGGCCGTGGTGGC
  AGATGATGGTATCACACAGTATGCTGTGCTCGGTGCAGGGCTCTACTTTGGGGAGATCAGCATCATCAAC
  ATCAAAGGGAACATGTCTGGGAACCGCCGCACAGCCAACATCAAGAGCCTAGGTTATTCAGACCTATTCT
  GCCTGAGCAAGGAGGACCTGCGGGAGGTGCTGAGCGAGTATCCACAAGCACAGACCATCATGGAGGAGAA
  AGGACGTGAGATCCTGCTGAAAATGAACAAGTTGGACGTGAATGCTGAGGCAGCTGAGATCGCCCTGCAG
  GAGGCCACAGAGTCCCGGCTACGAGGCCTAGACCAGCAGCTGGATGATCTACAGACCAAGTTTGCTCGCC
  TCCTGGCTGAGCTGGAGTCCAGCGCACTTAAGATTGCTTACCGCATTGAACGGCTGGAGTGGCAGACTCG
  AGAGTGGCCAATGCCCGAGGACCTGGCTGAGGCTGATGACGAGGGTGAGCCTGAGGAGGGAACTTCCAAA
  GATGAAGAGGGCAGGGCCAGCCAGGAGGGACCCCCAGGTCCAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218174 representing NM_001037329
  Red=Cloning site Green=Tags(s)
  
  MSQDTKVKTTESSPPAPSKARKLLPVLDPSGDYYYWWLNTMVFPVMYNLIILVCRACFPDLQHGYLVAWL
  VLDYTSDLLYLLDMVVRFHTGFLEQGILVVDKGRISSRYVRTWSFFLDLASLMPTDVVYVRLGPHTPTLR
  LNRFLRAPRLFEAFDRTETRTAYPNAFRIAKLMLYIFVVIHWNSCLYFALSRYLGFGRDAWVYPDPAQPG
  FERLRRQYLYSFYFSTLILTTVGDTPPPAREEEYLFMVGDFLLAVMGFATIMGSMSSVIYNMNTADAAFY
  PDHALVKKYMKLQHVNRKLERRVIDWYQHLQINKKMTNEVAILQHLPERLRAEVAVSVHLSTLSRVQIFQ
  NCEASLLEELVLKLQPQTYSPGEYVCRKGDIGQEMYIIREGQLAVVADDGITQYAVLGAGLYFGEISIIN
  IKGNMSGNRRTANIKSLGYSDLFCLSKEDLREVLSEYPQAQTIMEEKGREILLKMNKLDVNAEAAEIALQ
  EATESRLRGLDQQLDDLQTKFARLLAELESSALKIAYRIERLEWQTREWPMPEDLAEADDEGEPEEGTSK
  DEEGRASQEGPPGPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037329
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037329.4
• RefSeq Size: 1851 bp
• RefSeq ORF: 1728 bp
• Locus ID: 1262
• UniProt ID: Q8IV77
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 65.8 kDa
• Gene Summary: CNGA4 is a modulatory subunit of vertebrate cyclic nucleotide-gated membrane channels that transduce odorant signals (Munger et al., 2001 [PubMed 11739959]).[supplied by OMIM, Mar 2008]