Ankyrin repeat domain containing protein 33 (ANKRD33) (NM_182608) Human Tagged ORF Clone

CAT#: RC218015

ANKRD33 (Myc-DDK-tagged)-Human ankyrin repeat domain 33 (ANKRD33), transcript variant 2

Product Images

Circular map for RC218015

• 

  Western blot validation of overexpression lysate (Cat# LY405454) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218015 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankyrin repeat domain containing protein 33
• Synonyms: C12orf7; PANKY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218015 representing NM_182608
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTCCAGCCATCTGTTACCTGCGTTGCTTCCTGGGGAGGGATAGTCCACCTGGAGGCATTCGGAG
  ACCCAGTGATTGTGCTCCGCGGAGCCTGGGCTGTGCCCCGCGTTGACTGCCTCATAGATACCCTACGAAC
  CCCAAATGCCAGCTGCATGAGAAAAGGGACTCACCTTCTGGTTCCCTGCCTGGAAGAGGAAGAGCTGGCA
  TTGCACAGGAGACGGCTGGACATGTCTGAGGCACTGCCCTGCCCGGGCAAGGAGACCCCCACCCCAGGCT
  GCAGGCTGGGGGCCCTGTATTGGGCCTGTGTCCACAATGATCCCACCCAGCTCCAAGCCATACTGGATGG
  TGGGGTCTCCCCAGAGGAGGCCACCCAGGTGGACAGCAATGGGAGGACAGGCCTCATGGTCGCATGCTAC
  CACGGCTTCCAGAGTGTTGTGGCCCTGCTCAGCCACTGTCCTTTCCTTGATGTGAACCAGCAGGACAAAG
  GAGGGGACACGGCCCTCATGTTGGCTGCCCAAGCAGGCCACGTGCCTCTAGTGAGTCTCCTGCTCAACTA
  CTATGTGGGCCTGGACCTGGAACGCCGGGACCAGCGGGGGCTCACGGCGTTAATGAAGGCTGCCATGCGG
  AACCGCTGTGCTGACCTGACAGCAGTGGACCCTGTTCGGGGCAAGACGGCCCTGGAATGGGCAGTGCTGA
  CCGACAGCTTCGACACCGTGTGGAGGATTCGGCAGCTGCTGAGGCGGCCCCAAGTGGAGCAGCTTAGCCA
  GCACTACAAGCCCGAGTGGCCGGCCTTGTCCGGGCTCGTGGCCCAGGCCCAGGCCCAGGCCCAGGTTGCC
  CCTTCACTCCTAGAACGGCTGCAGGCTACCTTGAGCCTCCCCTTTGCCCCGTCTCCTCAGGAGGGGGGTG
  TTCTGGACCACCTTGTGACTGCCACAACCAGCCTGGCCAGTCCCTTCGTCACCACTGCCTGCCACACTCT
  GTGCCCTGACCATCCACCTTCGCTGGGCACCCGAAGCAAGTCCGTGCCAGAGCTGTTAGGTACTGCCCCG
  CCCCCTCCCCTGGTTCCCCAGTCCCCGCCAGGGAGTCCCCAGAGGTCCCCGTGGGTCTTCGTCCCCTACC
  AGAGCCCTCAGGGCATATTGAGCAAGTGCCTTCAGTGGCTACAACCCAGGGATAGCACCAGCCCCAGGCC
  CCAAGTCCCCAAGATCCTCCTCTCCAAGGCATCCTCATCCTCCCACCAGTGCCAGCCGAAGCCCAGTCCT
  TCAGGACACCAAAGTCTGGCCCTTCCTCTCTGGCGATACCAGGAGCTCAGGATAGAGAAGAGGAAACAGG
  AGGAGGAGGCCAGAATGGCACAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218015 representing NM_182608
  Red=Cloning site Green=Tags(s)
  
  MKVQPSVTCVASWGGIVHLEAFGDPVIVLRGAWAVPRVDCLIDTLRTPNASCMRKGTHLLVPCLEEEELA
  LHRRRLDMSEALPCPGKETPTPGCRLGALYWACVHNDPTQLQAILDGGVSPEEATQVDSNGRTGLMVACY
  HGFQSVVALLSHCPFLDVNQQDKGGDTALMLAAQAGHVPLVSLLLNYYVGLDLERRDQRGLTALMKAAMR
  NRCADLTAVDPVRGKTALEWAVLTDSFDTVWRIRQLLRRPQVEQLSQHYKPEWPALSGLVAQAQAQAQVA
  PSLLERLQATLSLPFAPSPQEGGVLDHLVTATTSLASPFVTTACHTLCPDHPPSLGTRSKSVPELLGTAP
  PPPLVPQSPPGSPQRSPWVFVPYQSPQGILSKCLQWLQPRDSTSPRPQVPKILLSKASSSSHQCQPKPSP
  SGHQSLALPLWRYQELRIEKRKQEEEARMAQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182608
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182608.4
• RefSeq Size: 1935 bp
• RefSeq ORF: 1359 bp
• Locus ID: 341405
• UniProt ID: Q7Z3H0
• Cytogenetics: 12q13.13
• MW: 49.2 kDa
• Gene Summary: Acts as a transcriptional repressor for CRX-activated photoreceptor gene regulation.[UniProtKB/Swiss-Prot Function]