SCFD1 (NM_016106) Human Tagged ORF Clone

CAT#: RC217990

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SCFD1 (Myc-DDK-tagged)-Human sec1 family domain containing 1 (SCFD1), transcript variant 1



Reconstitution Protocol

USD 598.00

USD 896.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SCFD1
Synonyms C14orf163; RA410; SLY1; SLY1P; STXBP1L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217990 representing NM_016106
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGGCGGCAGCGACAGCAGCAGCAGCAGCCAGTATTCGGGAAAGGCAGACAGTGGCTTTGA
AGCGTATGTTGAATTTCAATGTGCCTCATATTAAAAACAGCACAGGAGAACCAGTATGGAAGGTACTCAT
TTATGACAGATTTGGCCAAGATATAATCTCTCCTCTGCTATCTGTGAAGGAGCTAAGAGACATGGGAATC
ACTCTGCATCTGCTTTTACACTCTGATCGAGATCCTATTCCAGATGTTCCTGCAGTATACTTTGTAATGC
CAACTGAAGAAAATATTGACAGAATGTGCCAGGATCTTCGAAATCAACTATATGAATCATATTATTTAAA
TTTTATTTCTGCTATTTCAAGAAGTAAACTGGAAGATATTGCAAATGCAGCGTTAGCAGCTAGTGCAGTA
ACACAAGTAGCCAAGGTTTTTGACCAATATCTCAATTTTATTACTTTGGAAGATGATATGTTTGTATTAT
GTAATCAAAATAAGGAGCTTGTTTCATATCGTGCCATTAACAGGCCAGATATCACAGACACGGAAATGGA
AACTGTTATGGACACTATAGTTGACAGCCTCTTCTGCTTTTTTGTTACTCTGGGTGCTGTTCCTATAATC
AGATGTTCAAGAGGAACAGCAGCAGAAATGGTAGCAGTGAAACTAGACAAGAAACTTCGAGAAAATCTAA
GAGATGCAAGAAACAGTCTTTTTACAGGTGATACACTTGGAGCTGGCCAATTCAGCTTCCAGAGGCCCTT
ATTAGTCCTTGTTGACAGAAACATAGATTTGGCAACTCCTTTACATCATACTTGGACATATCAAGCATTG
GTGCACGATGTACTGGATTTCCATTTAAACAGGGTTAATTTGGAAGAATCTTCAGGAGTGGAAAACTCTC
CAGCTGGTGCTAGACCAAAGAGAAAAAACAAGAAGTCTTATGATTTAACTCCGGTTGATAAATTTTGGCA
AAAACATAAAGGAAGTCCATTCCCAGAAGTTGCAGAATCAGTTCAGCAAGAACTAGAATCTTACAGAGCA
CAGGAAGATGAGGTCAAACGACTTAAAAGCATTATGGGACTAGAAGGGGAAGATGAAGGAGCCATAAGTA
TGCTTTCTGACAATACCGCTAAGCTAACATCAGCTGTTAGTTCTTTGCCAGAACTCCTTGAGAAAAAAAG
ACTTATTGATCTCCATACAAATGTTGCCACTGCTGTTTTAGAACATATAAAGGCAAGAAAATTGGATGTA
TATTTTGAATATGAAGAAAAAATAATGAGCAAAACTACTCTGGATAAATCTCTTCTAGATATAATATCAG
ACCCTGATGCAGGAACTCCAGAAGATAAAATGAGGTTGTTTCTTATCTATTATATAAGCACACAGCAAGC
ACCTTCTGAGGCTGATTTGGAGCAATATAAAAAAGCTTTAACTGATGCAGGATGCAACCTTAATCCTTTA
CAATATATCAAACAGTGGAAGGCTTTTACCAAGATGGCCTCAGCTCCGGCCAGCTATGGCAGCACTACCA
CTAAACCAATGGGTCTTTTATCACGAGTCATGAATACAGGATCACAGTTTGTGATGGAAGGAGTGAAGAA
CCTGGTTTTGAAACAGCAAAATCTACCTGTTACTCGTATTTTGGACAATCTTATGGAGATGAAGTCAAAC
CCCGAAACTGATGACTATAGATATTTTGATCCCAAAATGCTGCGGGGCAATGACAGCTCAGTTCCCAGAA
ATAAAAATCCATTCCAAGAGGCCATTGTTTTTGTGGTGGGAGGAGGCAACTACATTGAATATCAGAATCT
TGTTGACTACATAAAGGGGAAACAAGGCAAACACATTTTATATGGCTGCAGTGAGCTTTTTAATGCTACA
CAGTTCATAAAACAGTTGTCACAACTTGGACAAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217990 representing NM_016106
Red=Cloning site Green=Tags(s)

MAAAAAATAAAAASIRERQTVALKRMLNFNVPHIKNSTGEPVWKVLIYDRFGQDIISPLLSVKELRDMGI
TLHLLLHSDRDPIPDVPAVYFVMPTEENIDRMCQDLRNQLYESYYLNFISAISRSKLEDIANAALAASAV
TQVAKVFDQYLNFITLEDDMFVLCNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPII
RCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDTLGAGQFSFQRPLLVLVDRNIDLATPLHHTWTYQAL
VHDVLDFHLNRVNLEESSGVENSPAGARPKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRA
QEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDV
YFEYEEKIMSKTTLDKSLLDIISDPDAGTPEDKMRLFLIYYISTQQAPSEADLEQYKKALTDAGCNLNPL
QYIKQWKAFTKMASAPASYGSTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSN
PETDDYRYFDPKMLRGNDSSVPRNKNPFQEAIVFVVGGGNYIEYQNLVDYIKGKQGKHILYGCSELFNAT
QFIKQLSQLGQK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016106
ORF Size 1926 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016106.4
RefSeq Size 2172 bp
RefSeq ORF 1929 bp
Locus ID 23256
UniProt ID Q8WVM8
Cytogenetics 14q12
Domains Sec1
MW 72.2 kDa
Gene Summary Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).[UniProtKB/Swiss-Prot Function]
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