SCFD1 (NM_016106) Human Tagged ORF Clone

CAT#: RC217990

SCFD1 (Myc-DDK-tagged)-Human sec1 family domain containing 1 (SCFD1), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SCFD1 (Cat# RC217990, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCFD1(Cat# TA504721). Positive lysates LY414181 (100ug) and LC414181 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414181) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217990 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SCFD1 protein (Cat# TP317990). The protein was produced from HEK293T cells transfected with SCFD1 cDNA clone (Cat# RC217990) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCFD1
• Synonyms: C14orf163; RA410; SLY1; SLY1P; STXBP1L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217990 representing NM_016106
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCAGCGACAGCAGCAGCAGCAGCCAGTATTCGGGAAAGGCAGACAGTGGCTTTGA
  AGCGTATGTTGAATTTCAATGTGCCTCATATTAAAAACAGCACAGGAGAACCAGTATGGAAGGTACTCAT
  TTATGACAGATTTGGCCAAGATATAATCTCTCCTCTGCTATCTGTGAAGGAGCTAAGAGACATGGGAATC
  ACTCTGCATCTGCTTTTACACTCTGATCGAGATCCTATTCCAGATGTTCCTGCAGTATACTTTGTAATGC
  CAACTGAAGAAAATATTGACAGAATGTGCCAGGATCTTCGAAATCAACTATATGAATCATATTATTTAAA
  TTTTATTTCTGCTATTTCAAGAAGTAAACTGGAAGATATTGCAAATGCAGCGTTAGCAGCTAGTGCAGTA
  ACACAAGTAGCCAAGGTTTTTGACCAATATCTCAATTTTATTACTTTGGAAGATGATATGTTTGTATTAT
  GTAATCAAAATAAGGAGCTTGTTTCATATCGTGCCATTAACAGGCCAGATATCACAGACACGGAAATGGA
  AACTGTTATGGACACTATAGTTGACAGCCTCTTCTGCTTTTTTGTTACTCTGGGTGCTGTTCCTATAATC
  AGATGTTCAAGAGGAACAGCAGCAGAAATGGTAGCAGTGAAACTAGACAAGAAACTTCGAGAAAATCTAA
  GAGATGCAAGAAACAGTCTTTTTACAGGTGATACACTTGGAGCTGGCCAATTCAGCTTCCAGAGGCCCTT
  ATTAGTCCTTGTTGACAGAAACATAGATTTGGCAACTCCTTTACATCATACTTGGACATATCAAGCATTG
  GTGCACGATGTACTGGATTTCCATTTAAACAGGGTTAATTTGGAAGAATCTTCAGGAGTGGAAAACTCTC
  CAGCTGGTGCTAGACCAAAGAGAAAAAACAAGAAGTCTTATGATTTAACTCCGGTTGATAAATTTTGGCA
  AAAACATAAAGGAAGTCCATTCCCAGAAGTTGCAGAATCAGTTCAGCAAGAACTAGAATCTTACAGAGCA
  CAGGAAGATGAGGTCAAACGACTTAAAAGCATTATGGGACTAGAAGGGGAAGATGAAGGAGCCATAAGTA
  TGCTTTCTGACAATACCGCTAAGCTAACATCAGCTGTTAGTTCTTTGCCAGAACTCCTTGAGAAAAAAAG
  ACTTATTGATCTCCATACAAATGTTGCCACTGCTGTTTTAGAACATATAAAGGCAAGAAAATTGGATGTA
  TATTTTGAATATGAAGAAAAAATAATGAGCAAAACTACTCTGGATAAATCTCTTCTAGATATAATATCAG
  ACCCTGATGCAGGAACTCCAGAAGATAAAATGAGGTTGTTTCTTATCTATTATATAAGCACACAGCAAGC
  ACCTTCTGAGGCTGATTTGGAGCAATATAAAAAAGCTTTAACTGATGCAGGATGCAACCTTAATCCTTTA
  CAATATATCAAACAGTGGAAGGCTTTTACCAAGATGGCCTCAGCTCCGGCCAGCTATGGCAGCACTACCA
  CTAAACCAATGGGTCTTTTATCACGAGTCATGAATACAGGATCACAGTTTGTGATGGAAGGAGTGAAGAA
  CCTGGTTTTGAAACAGCAAAATCTACCTGTTACTCGTATTTTGGACAATCTTATGGAGATGAAGTCAAAC
  CCCGAAACTGATGACTATAGATATTTTGATCCCAAAATGCTGCGGGGCAATGACAGCTCAGTTCCCAGAA
  ATAAAAATCCATTCCAAGAGGCCATTGTTTTTGTGGTGGGAGGAGGCAACTACATTGAATATCAGAATCT
  TGTTGACTACATAAAGGGGAAACAAGGCAAACACATTTTATATGGCTGCAGTGAGCTTTTTAATGCTACA
  CAGTTCATAAAACAGTTGTCACAACTTGGACAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217990 representing NM_016106
  Red=Cloning site Green=Tags(s)
  
  MAAAAAATAAAAASIRERQTVALKRMLNFNVPHIKNSTGEPVWKVLIYDRFGQDIISPLLSVKELRDMGI
  TLHLLLHSDRDPIPDVPAVYFVMPTEENIDRMCQDLRNQLYESYYLNFISAISRSKLEDIANAALAASAV
  TQVAKVFDQYLNFITLEDDMFVLCNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPII
  RCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDTLGAGQFSFQRPLLVLVDRNIDLATPLHHTWTYQAL
  VHDVLDFHLNRVNLEESSGVENSPAGARPKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRA
  QEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDV
  YFEYEEKIMSKTTLDKSLLDIISDPDAGTPEDKMRLFLIYYISTQQAPSEADLEQYKKALTDAGCNLNPL
  QYIKQWKAFTKMASAPASYGSTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSN
  PETDDYRYFDPKMLRGNDSSVPRNKNPFQEAIVFVVGGGNYIEYQNLVDYIKGKQGKHILYGCSELFNAT
  QFIKQLSQLGQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016106
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016106.4
• RefSeq Size: 2172 bp
• RefSeq ORF: 1929 bp
• Locus ID: 23256
• UniProt ID: Q8WVM8
• Cytogenetics: 14q12
• Domains: Sec1
• MW: 72.2 kDa
• Gene Summary: Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).[UniProtKB/Swiss-Prot Function]