OR10Z1 (NM_001004478) Human Tagged ORF Clone

CAT#: RC217906

OR10Z1 (Myc-DDK-tagged)-Human olfactory receptor, family 10, subfamily Z, member 1 (OR10Z1)

Product Images

Circular map for RC217906

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  Western blot validation of overexpression lysate (Cat# LY423781) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217906 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR10Z1
• Synonyms: OR1-15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217906 representing NM_001004478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGACCAACGTAACCTCCTGGAGGGATTTTGTCTTCCTGGGCTTCTCCAGTTCTGGGGAGTTGC
  AGCTCCTTCTCTTTGCCTTGTTCCTCTCTCTGTATCTAGTCACTCTGACCAGCAATGTCTTCATTATCAT
  AGCCATCAGGCTGGATAGCCATCTGCACACCCCCATGTACCTCTTCCTTTCCTTCCTATCCTTCTCTGAG
  ACCTGCTACACTTTGGGCATCATCCCTAGAATGCTCTCTGGCCTGGCTGGGGGGGACCAGGCTATCTCCT
  ATGTGGGCTGTGCTGCCCAGATGTTCTTTTCTGCCTCATGGGCCTGTACTAACTGCTTCCTTCTGGCTGC
  CATGGGCTTTGACAGATATGTGGCCATCTGTGCTCCACTCCACTATGCCAGCCACATGAATCCTACCCTC
  TGTGCCCAGCTGGTCATTACTTCCTTCCTGACTGGATACCTCTTTGGACTGGGAATGACACTAGTTATTT
  TCCACCTCTCATTCTGCAGCTCCCATGAAATCCAGCACTTTTTTTGTGACACGCCACCTGTGCTGAGCCT
  AGCCTGTGGAGATACAGGCCCGAGTGAGCTGAGGATCTTTATCCTCAGTCTTTTGGTCCTCTTGGTCTCC
  TTCTTCTTCATCACCATCTCCTACGCCTACATCTTGGCAGCAATACTGAGGATCCCCTCTGCTGAGGGGC
  AGAAGAAGGCCTTCTCCACTTGTGCCTCGCACCTTACAGTGGTCATTATTCATTATGGCTGTGCTTCCTT
  CGTGTACCTGAGGCCCAAAGCCAGCTACTCTCTTGAGAGAGATCAGCTTATTGCCATGACCTATACTGTA
  GTGACCCCCCTCCTTAATCCCATTGTTTATAGTCTAAGGAATAGGGCTATACAGACAGCTCTGAGGAATG
  CTTTCAGAGGGAGATTGCTGGGTAAAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217906 representing NM_001004478
  Red=Cloning site Green=Tags(s)
  
  MGQTNVTSWRDFVFLGFSSSGELQLLLFALFLSLYLVTLTSNVFIIIAIRLDSHLHTPMYLFLSFLSFSE
  TCYTLGIIPRMLSGLAGGDQAISYVGCAAQMFFSASWACTNCFLLAAMGFDRYVAICAPLHYASHMNPTL
  CAQLVITSFLTGYLFGLGMTLVIFHLSFCSSHEIQHFFCDTPPVLSLACGDTGPSELRIFILSLLVLLVS
  FFFITISYAYILAAILRIPSAEGQKKAFSTCASHLTVVIIHYGCASFVYLRPKASYSLERDQLIAMTYTV
  VTPLLNPIVYSLRNRAIQTALRNAFRGRLLGKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004478
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004478.1, NP_001004478.1
• RefSeq Size: 942 bp
• RefSeq ORF: 942 bp
• Locus ID: 128368
• UniProt ID: Q8NGY1
• Cytogenetics: 1q23.1
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 34.4 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]