Phospholipase C beta 4 (PLCB4) (NM_182797) Human Tagged ORF Clone

CAT#: RC217903

PLCB4 (Myc-DDK-tagged)-Human phospholipase C, beta 4 (PLCB4), transcript variant 2

Product Images

Circular map for RC217903

• 

  Western blot validation of overexpression lysate (Cat# LY405361) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217903 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phospholipase C beta 4
• Synonyms: ARCND2; PI-PLC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217903 representing NM_182797
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAACCTTATGAATTTAACTGGCAGAAGGAAGTTCCCTCCTTTTTGCAAGAAGGAGCAGTTTTTG
  ACAGATACGAGGAGGAATCCTTTGTGTTTGAACCCAACTGCCTCTTCAAAGTGGATGAGTTTGGCTTCTT
  TCTGACATGGAGAAGTGAAGGCAAGGAAGGACAGGTGCTAGAATGCTCCCTCATCAACAGTATTCGGTCG
  GGAGCCATACCAAAGGATCCCAAAATCTTGGCTGCTCTTGAAGCTGTTGGAAAATCAGAAAATGATCTGG
  AAGGGCGGATAGTTTGTGTCTGCAGTGGCACAGATCTAGTGAACATTAGTTTTACCTACATGGTGGCTGA
  AAATCCAGAAGTAACTAAGCAATGGGTAGAAGGCCTGAGATCAATCATACACAACTTCAGGGCCAACAAC
  GTCAGTCCAATGACATGCCTCAAGAAACACTGGATGAAATTGGCATTTATGACCAACACAAATGGTAAAA
  TTCCAGTTAGGAGTATTACTAGAACATTTGCATCGGGAAAAACAGAAAAGGTGATCTTTCAAGCACTCAA
  GGAGTTAGGTCTTCCCAGTGGAAAGAATGATGAAATTGAGCCCACAGCATTTTCTTATGAAAAGTTCTAT
  GAACTGACACAAAAGATTTGTCCTCGGACAGATATAGAAGATCTTTTCAAAAAAATCAATGGAGACAAAA
  CTGATTATTTAACGGTAGACCAATTAGTGAGCTTTCTAAATGAACATCAACGAGATCCTCGATTGAATGA
  AATTTTATTTCCATTTTATGATGCCAAAAGGGCAATGCAGATCATTGAGATGTATGAACCTGATGAAGAT
  TTGAAGAAAAAAGGCCTTATATCAAGTGATGGGTTTTGCAGATATCTGATGTCAGATGAAAACGCCCCAG
  TCTTCCTAGATCGTTTAGAACTTTACCAAGAAATGGACCATCCTCTGGCTCACTACTTCATCAGTTCTTC
  CCATAACACTTATCTCACTGGCAGACAGTTCGGCGGGAAGTCTTCGGTAGAAATGTACAGACAGGTTCTC
  CTGGCTGGTTGCAGATGTGTTGAACTTGACTGCTGGGATGGAAAAGGTGAAGACCAAGAACCAATAATAA
  CTCATGGAAAAGCAATGTGTACAGATATCCTTTTTAAGGATGTAATTCAAGCCATCAAGGAAACTGCATT
  TGTCACATCAGAATATCCTGTAATTCTCTCCTTTGAAAATCACTGCAGCAAATATCAACAGTACAAGATG
  TCCAAATATTGCGAAGATCTATTTGGGGATCTCCTGTTGAAACAAGCACTTGAATCACATCCACTTGAAC
  CAGGCAGGGCTTTGCCATCCCCCAATGACCTCAAAAGAAAAATACTCATAAAAAACAAGCGGCTGAAACC
  TGAAGTTGAAAAAAAACAGCTGGAAGCTTTGAGAAGCATGATGGAAGCTGGAGAATCTGCCTCCCCAGCA
  AACATCTTAGAGGACGATAATGAAGAGGAGATCGAAAGTGCTGACCAAGAGGAGGAAGCTCACCCCGAAT
  TCAAATTTGGAAATGAACTTTCTGCTGATGACTTGGGTCACAAGGAAGCTGTTGCAAATAGCGTCAAGAA
  GGGCCTGGTCACTGTAGAAGATGAGCAGGCGTGGATGGCATCTTATAAATATGTAGGTGCTACCACTAAT
  ATCCATCCATATTTGTCCACAATGATCAACTACGCCCAGCCTGTAAAGTTTCAAGGTTTCCATGTGGCAG
  AAGAACGCAATATTCATTATAACATGTCTTCTTTTAATGAATCAGTCGGTCTTGGCTACTTGAAGACACA
  TGCAATTGAATTTGTCAATTATAACAAACGGCAAATGAGTCGCATTTACCCCAAGGGAGGCCGAGTCGAT
  TCCAGTAATTACATGCCTCAGATTTTCTGGAACGCTGGCTGCCAGATGGTTTCACTGAACTATCAAACCC
  CAGATTTAGCGATGCAATTGAATCAGGGAAAATTTGAGTATAATGGATCGTGCGGGTACCTTCTCAAACC
  AGATTTCATGAGGCGGCCTGATCGAACATTTGACCCCTTCTCTGAAACTCCTGTTGATGGTGTTATTGCA
  GCCACTTGCTCAGTGCAGGTTATATCAGGTCAATTCTTATCAGATAAGAAAATTGGCACCTACGTAGAGG
  TGGATATGTATGGGTTGCCCACTGACACCATACGTAAGGAATTCCGAACTCGCATGGTTATGAATAATGG
  ACTCAATCCAGTTTACAATGAAGAGTCATTTGTATTTCGGAAGGTGATCCTGCCGGACCTGGCTGTCTTG
  AGAATAGCTGTGTATGATGATAACAACAAGCTGATTGGCCAGAGGATCCTCCCGCTTGATGGCCTCCAAG
  CCGGATATCGACACATTTCCCTTCGAAATGAGGGAAATAAACCATTATCACTACCAACAATTTTCTGCAA
  TATTGTTCTTAAAACATATGTGCCTGATGGATTTGGAGATATCGTGGATGCTTTATCAGATCCAAAGAAA
  TTTCTCTCAATTACAGAAAAGAGAGCAGACCAAATGAGAGCTATGGGCATTGAAACTAGTGACATAGCCG
  ACGTGCCCAGTGACACTTCCAAAAATGACAAGAAAGGAAAGGCCAACACCGCCAAAGCAAATGTGACCCC
  TCAGAGTAGCTCTGAGCTCAGACCAACCACCACGGCTGCCCTGGCCTCTGGTGTGGAAGCCAAGAAAGGT
  ATTGAACTTATCCCTCAAGTAAGGATAGAAGACTTAAAGCAGATGAAGGCTTACTTGAAGCATTTAAAGA
  AACAGCAGAAGGAGCTAAATTCTTTAAAGAAGAAACATGCAAAGGAACACAGTACCATGCAGAAGTTACA
  CTGCACGCAAGTTGACAAAATTGTGGCACAGTATGACAAAGAGAAGTCGACTCATGAGAAAATCCTAGAG
  AAGGCAATGAAGAAGAAGGGGGGAAGTAATTGTCTCGAAATGAAAAAAGAAACAGAAATCAAAATTCAGA
  CGCTGACATCAGATCACAAATCTAAGGTCAAAGAGATTGTAGCACAGCACACAAAGGAATGGTCAGAAAT
  GATCAATACCCACAGTGCTGAGGAGCAAGAAATCCGAGACCTGCACCTCAGCCAGCAGTGTGAGCTGCTG
  AAAAAGCTACTCATCAATGCCCACGAGCAGCAAACCCAGCAGCTGAAACTGTCCCATGACAGGGAAAGCA
  AGGAAATGCGAGCACACCAGGCTAAGATTTCTATGGAAAATAGCAAAGCCATCAGCCAAGATAAATCTAT
  CAAGAATAAAGCAGAACGGGAAAGGCGAGTCAGGGAGTTAAACAGCAGCAACACTAAAAAGTTTCTGGAA
  GAAAGAAAGAGACTTGCCATGAAGCAGTCCAAAGAAATGGATCAGTTGAAAAAAGTCCAGCTTGAACATC
  TAGAATTCCTAGAGAAACAGAATGAGCAGGCGAAGGAGATGCAGCAGATGGTGAAATTGGAAGCCGAGAT
  GGACCGCAGACCAGCAACAGTAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217903 representing NM_182797
  Red=Cloning site Green=Tags(s)
  
  MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSIRS
  GAIPKDPKILAALEAVGKSENDLEGRIVCVCSGTDLVNISFTYMVAENPEVTKQWVEGLRSIIHNFRANN
  VSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPTAFSYEKFY
  ELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDED
  LKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVL
  LAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKM
  SKYCEDLFGDLLLKQALESHPLEPGRALPSPNDLKRKILIKNKRLKPEVEKKQLEALRSMMEAGESASPA
  NILEDDNEEEIESADQEEEAHPEFKFGNELSADDLGHKEAVANSVKKGLVTVEDEQAWMASYKYVGATTN
  IHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVD
  SSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIA
  ATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVL
  RIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKK
  FLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALASGVEAKKG
  IELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILE
  KAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMINTHSAEEQEIRDLHLSQQCELL
  KKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLE
  ERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182797
• ORF Size: 3525 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182797.3
• RefSeq Size: 5509 bp
• RefSeq ORF: 3528 bp
• Locus ID: 5332
• UniProt ID: Q15147
• Cytogenetics: 20p12.3-p12.2
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway
• MW: 134.3 kDa
• Gene Summary: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010]