TACC1 (NM_006283) Human Tagged ORF Clone

CAT#: RC217791

TACC1 (Myc-DDK-tagged)-Human transforming, acidic coiled-coil containing protein 1 (TACC1), transcript variant 1

Product Images

Circular map for RC217791

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TACC1 (Cat# RC217791, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC1(Cat# TA590577).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TACC1
• Synonyms: Ga55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217791 representing NM_006283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCAGCCCGTGGCAGATCCTGTCCCCCGTGCAGTGGGCGAAATGGACGTGGTCTGCGGTACGCG
  GCGGGGCCGCCGGCGAGGACGAGGCTGGCGGGCCCGAGGGCGACCCCGAGGAGGAGGATTCGCAAGCCGA
  GACCAAATCCTTGAGTTTCAGCTCGGATTCTGAAGGTAATTTTGAGACTCCTGAAGCTGAAACCCCGATC
  CGATCACCTTTCAAGGAGTCCTGTGATCCATCACTCGGATTGGCAGGACCTGGGGCCAAAAGCCAAGAAT
  CACAAGAAGCTGATGAACAGCTTGTAGCAGAAGTGGTTGAAAAATGTTCATCTAAGACTTGTTCTAAACC
  TTCAGAAAATGAAGTGCCACAGCAGGCCATTGACTCTCACTCAGTCAAGAATTTCAGAGAAGAACCTGAA
  CATGATTTTAGCAAAATTTCCATCGTGAGGCCATTTTCAATAGAAACGAAGGATTCCACGGATATCTCGG
  CAGTCCTCGGAACAAAAGCAGCTCATGGCTGTGTAACTGCAGTCTCAGGCAAGGCTCTGCCTTCCAGCCC
  GCCAGACGCCCTCCAGGACGAGGCGATGACAGAAGGCAGCATGGGGGTCACCCTCGAGGCCTCCGCAGAA
  GCTGATCTAAAAGCTGGCAACTCCTGTCCAGAGCTTGTGCCCAGCAGAAGAAGCAAGCTGAGAAAGCCCA
  AGCCTGTCCCCCTGAGGAAGAAAGCAATTGGAGGAGAGTTCTCAGACACCAACGCTGCTGTGGAGGGCAC
  ACCTCTCCCCAAGGCATCCTATCACTTCAGTCCTGAAGAGTTGGATGAGAACACAAGTCCTTTGCTAGGA
  GATGCCAGGTTCCAGAAGTCTCCCCCTGACATTAAAGAAACTCCCGGCACTCTCAGTAGTGACACCAACG
  ACTCAGGGGTTGAGCTGGGGGAGGAGTCGAGGAGCTCACCTCTCAAGCTTGAGTTTGATTTCACAGAAGA
  TACAGGAAACATAGAGGCCAGGAAAGCCCTTCCAAGGAAGCTTGGCAGGAAACTGGGTAGCACACTGACT
  CCCAAGATACAAAAAGATGGCATCAGTAAGTCAGCAGGTTTAGAACAGCCTACAGACCCAGTGGCACGAG
  ACGGGCCTCTCTCCCAAACATCTTCCAAGCCAGATCCTAGTCAGTGGGAGAGCCCCAGCTTCAACCCCTT
  TGGGAGCCACTCTGTTCTGCAGAACTCCCCACCCCTCTCTTCTGAGGGCTCCTACCACTTTGACCCAGAT
  AACTTTGACGAATCCATGGATCCCTTTAAACCAACTACGACCTTAACAAGCAGTGACTTTTGTTCTCCCA
  CTGGTAATCACGTTAATGAAATCTTAGAATCACCCAAGAAGGCAAAGTCGCGTTTAATAACGAGTGGCTG
  TAAGGTGAAGAAGCATGAAACTCAGTCTCTCGCCCTGGATGCATGTTCTCGGGATGAAGGGGCAGTGATC
  TCCCAGATTTCAGACATTTCTAATAGGGATGGCCATGCTACTGATGAGGAGAAACTGGCATCCACGTCAT
  GTGGTCAGAAATCAGCTGGTGCCGAGGTGAAAGGTGAGCCAGAGGAAGACCTGGAGTACTTTGAATGTTC
  CAATGTTCCTGTGTCTACCATAAATCATGCGTTTTCATCCTCAGAAGCAGGCATAGAGAAGGAGACGTGC
  CAGAAGATGGAAGAAGACGGGTCCACTGTGCTTGGGCTGCTGGAGTCCTCTGCAGAGAAGGCCCCTGTGT
  CGGTGTCCTGTGGAGGTGAGAGCCCCCTGGATGGGATCTGCCTCAGCGAATCAGACAAGACAGCCGTGCT
  CACCTTAATAAGAGAAGAGATAATTACTAAAGAGATTGAAGCAAATGAATGGAAGAAGAAATACGAAGAG
  ACCCGGCAAGAAGTTTTGGAGATGAGGAAAATTGTAGCTGAATATGAAAAGACTATTGCTCAAATGATTG
  AAGATGAACAAAGGACAAGTATGACCTCTCAGAAGAGCTTCCAGCAACTGACCATGGAGAAGGAACAGGC
  CCTGGCTGACCTTAACTCTGTGGAAAGGTCCCTTTCTGATCTCTTCAGGAGATATGAGAACCTGAAAGGT
  GTTCTGGAAGGGTTCAAGAAGAATGAAGAAGCCTTGAAGAAATGTGCTCAGGATTACTTAGCCAGAGTTA
  AACAAGAGGAGCAGCGATACCAGGCCCTGAAAATCCACGCAGAAGAGAAACTGGACAAAGCCAATGAAGA
  GATTGCTCAGGTTCGAACAAAAGCAAAGGCTGAGAGTGCAGCTCTCCATGCTGGACTCCGCAAAGAGCAG
  ATGAAGGTGGAGTCCCTGGAAAGGGCCCTGCAGCAGAAGAACCAAGAAATTGAAGAACTGACAAAAATCT
  GTGATGAGCTGATTGCAAAGCTGGGAAAGACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217791 representing NM_006283
  Red=Cloning site Green=Tags(s)
  
  MAFSPWQILSPVQWAKWTWSAVRGGAAGEDEAGGPEGDPEEEDSQAETKSLSFSSDSEGNFETPEAETPI
  RSPFKESCDPSLGLAGPGAKSQESQEADEQLVAEVVEKCSSKTCSKPSENEVPQQAIDSHSVKNFREEPE
  HDFSKISIVRPFSIETKDSTDISAVLGTKAAHGCVTAVSGKALPSSPPDALQDEAMTEGSMGVTLEASAE
  ADLKAGNSCPELVPSRRSKLRKPKPVPLRKKAIGGEFSDTNAAVEGTPLPKASYHFSPEELDENTSPLLG
  DARFQKSPPDIKETPGTLSSDTNDSGVELGEESRSSPLKLEFDFTEDTGNIEARKALPRKLGRKLGSTLT
  PKIQKDGISKSAGLEQPTDPVARDGPLSQTSSKPDPSQWESPSFNPFGSHSVLQNSPPLSSEGSYHFDPD
  NFDESMDPFKPTTTLTSSDFCSPTGNHVNEILESPKKAKSRLITSGCKVKKHETQSLALDACSRDEGAVI
  SQISDISNRDGHATDEEKLASTSCGQKSAGAEVKGEPEEDLEYFECSNVPVSTINHAFSSSEAGIEKETC
  QKMEEDGSTVLGLLESSAEKAPVSVSCGGESPLDGICLSESDKTAVLTLIREEIITKEIEANEWKKKYEE
  TRQEVLEMRKIVAEYEKTIAQMIEDEQRTSMTSQKSFQQLTMEKEQALADLNSVERSLSDLFRRYENLKG
  VLEGFKKNEEALKKCAQDYLARVKQEEQRYQALKIHAEEKLDKANEEIAQVRTKAKAESAALHAGLRKEQ
  MKVESLERALQQKNQEIEELTKICDELIAKLGKTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006283
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006283.1, NP_006274.1
• RefSeq Size: 7758 bp
• RefSeq ORF: 2418 bp
• Locus ID: 6867
• UniProt ID: O75410
• Cytogenetics: 8p11.22
• Domains: TACC
• MW: 87.6 kDa
• Gene Summary: This locus may represent a breast cancer candidate gene. It is located close to FGFR1 on a region of chromosome 8 that is amplified in some breast cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]