QDPR (NM_000320) Human Tagged ORF Clone

CAT#: RC217737

QDPR (Myc-DDK-tagged)-Human quinoid dihydropteridine reductase (QDPR)

Product Images

Circular map for RC217737

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  Western blot validation of overexpression lysate (Cat# LY424801) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217737 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified QDPR protein (Cat# TP317737). The protein was produced from HEK293T cells transfected with QDPR cDNA clone (Cat# RC217737) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: QDPR
• Synonyms: DHPR; HDHPR; PKU2; SDR33C1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217737 representing NM_000320
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCTGCAGGCGAGGCGCGCCGGGTGCTGGTGTACGGCGGCAGGGGCGCTCTGGGTT
  CTCGATGCGTGCAGGCTTTTCGGGCCCGCAACTGGTGGGTTGCCAGCGTTGATGTGGTGGAGAATGAAGA
  GGCCAGCGCTAGCATCATTGTTAAAATGACAGACTCGTTCACTGAGCAGGCTGACCAGGTGACTGCTGAG
  GTTGGAAAGCTCTTGGGTGAAGAGAAGGTGGATGCAATTCTTTGCGTTGCTGGAGGATGGGCCGGGGGCA
  ATGCCAAATCCAAGTCTCTCTTTAAGAACTGTGACCTGATGTGGAAGCAGAGCATATGGACATCGACCAT
  CTCCAGCCATCTGGCTACCAAGCATCTCAAGGAAGGAGGCCTCCTGACCTTGGCTGGCGCAAAGGCTGCC
  CTGGATGGGACTCCTGGTATGATCGGGTACGGCATGGCCAAGGGTGCTGTTCACCAGCTCTGCCAGAGCC
  TGGCTGGGAAGAACAGCGGCATGCCGCCCGGGGCAGCCGCCATCGCTGTGCTCCCGGTTACCCTGGATAC
  CCCGATGAACAGGAAATCAATGCCTGAGGCTGACTTCAGCTCCTGGACACCCTTAGAATTCCTAGTTGAA
  ACTTTCCATGACTGGATCACAGGGAAAAACCGACCGAGCTCAGGAAGCCTAATCCAGGTGGTAACCACAG
  AAGGAAGGACGGAACTCACCCCAGCATATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217737 representing NM_000320
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAGEARRVLVYGGRGALGSRCVQAFRARNWWVASVDVVENEEASASIIVKMTDSFTEQADQVTAE
  VGKLLGEEKVDAILCVAGGWAGGNAKSKSLFKNCDLMWKQSIWTSTISSHLATKHLKEGGLLTLAGAKAA
  LDGTPGMIGYGMAKGAVHQLCQSLAGKNSGMPPGAAAIAVLPVTLDTPMNRKSMPEADFSSWTPLEFLVE
  TFHDWITGKNRPSSGSLIQVVTTEGRTELTPAYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000320
• ORF Size: 732 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000320.3
• RefSeq Size: 1550 bp
• RefSeq ORF: 735 bp
• Locus ID: 5860
• UniProt ID: P09417
• Cytogenetics: 4p15.32
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• MW: 25.6 kDa
• Gene Summary: This gene encodes the enzyme dihydropteridine reductase, which catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin. This enzyme is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. Mutations in this gene resulting in QDPR deficiency include aberrant splicing, amino acid substitutions, insertions, or premature terminations. Dihydropteridine reductase deficiency presents as atypical phenylketonuria due to insufficient production of biopterin, a cofactor for phenylalanine hydroxylase. [provided by RefSeq, Jul 2008]