OST beta (SLC51B) (NM_178859) Human Tagged ORF Clone

CAT#: RC217638

SLC51B (Myc-DDK-tagged)-Human organic solute transporter beta (OSTBETA)

Product Images

Circular map for RC217638

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  Western blot validation of overexpression lysate (Cat# LY405823) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217638 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SLC51B protein (Cat# TP317638). The protein was produced from HEK293T cells transfected with SLC51B cDNA clone (Cat# RC217638) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OST beta
• Synonyms: OSTB; OSTBETA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217638 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACAGTGAGGGGGCTCCCGGAGACCCAGCCGGTACTGTGGTACCCCAGGAGCTGCTGGAAGAGA
  TGCTTTGGTTTTTTCGTGTGGAAGATGCATCTCCCTGGAATCATTCCATCCTTGCCCTGGCAGCTGTGGT
  GGTCATTATAAGCATGGTCCTCCTGGGAAGAAGCATCCAGGCAAGCAGAAAAGAAAAGATGCAGCCACCA
  GAAAAAGAAACTCCAGAAGTCCTGCATTTGGATGAGGCCAAGGATCACAACAGCCTAAACAACCTAAGAG
  AAACTTTGCTCTCAGAAAAGCCAAACTTGGCCCAGGTGGAACTTGAGTTAAAAGAGAGAGATGTGCTGTC
  AGTTTTCCTTCCGGATGTACCAGAAACTGAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217638 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEHSEGAPGDPAGTVVPQELLEEMLWFFRVEDASPWNHSILALAAVVVIISMVLLGRSIQASRKEKMQPP
  EKETPEVLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPETES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178859
• ORF Size: 384 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178859.4
• RefSeq Size: 940 bp
• RefSeq ORF: 387 bp
• Locus ID: 123264
• UniProt ID: Q86UW2
• Cytogenetics: 15q22.31
• Protein Families: Transmembrane
• MW: 14.3 kDa
• Gene Summary: Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Modulates SLC51A glycosylation, membrane trafficking and stability activities.[UniProtKB/Swiss-Prot Function]