WIPF1 (NM_003387) Human Tagged ORF Clone

CAT#: RC217601

WIPF1 (Myc-DDK-tagged)-Human WAS/WASL interacting protein family, member 1 (WIPF1), transcript variant 1

Product Images

Circular map for RC217601

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WIPF1 (Cat# RC217601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WIPF1(Cat# TA590158). Positive lysates LY401163 (100ug) and LC401163 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401163) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217601 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WIPF1 protein (Cat# TP317601). The protein was produced from HEK293T cells transfected with WIPF1 cDNA clone (Cat# RC217601) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WIPF1
• Synonyms: PRPL-2; WAS2; WASPIP; WIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217601 representing NM_003387
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTCCCTCCCCCTCCAGCACCCCCGCCGCCCCCGACGTTTGCACTGGCCAATACAGAGAAGCCTA
  CCTTGAATAAGACAGAGCAGGCTGGGAGAAATGCTCTCCTTTCTGATATCAGCAAAGGGAAGAAACTAAA
  GAAGACGGTCACCAATGACAGAAGTGCACCAATACTGGACAAACCTAAAGGAGCTGGTGCTGGAGGCGGT
  GGTGGTGGCTTTGGTGGAGGCGGCGGATTTGGCGGAGGAGGTGGTGGCGGAGGCGGTGGAAGTTTTGGAG
  GGGGCGGACCTCCAGGTCTGGGAGGATTGTTCCAGGCTGGAATGCCGAAGCTGAGATCCACGGCCAACAG
  GGATAATGATTCTGGAGGAAGCCGACCACCATTGTTGCCACCGGGAGGAAGATCCACATCTGCGAAACCC
  TTTTCACCCCCAAGTGGCCCAGGGAGGTTTCCTGTGCCTTCTCCAGGCCACAGAAGTGGTCCCCCAGAGC
  CTCAGAGGAACCGAATGCCGCCCCCAAGGCCCGACGTGGGCTCAAAGCCTGATAGCATTCCTCCTCCAGT
  ACCTAGTACTCCAAGACCCATTCAATCAAGTCTGCACAACCGGGGGTCCCCACCAGTGCCCGGAGGCCCC
  AGGCAGCCCAGCCCCGGGCCCACTCCTCCCCCTTTCCCTGGAAACCGCGGCACTGCTTTGGGAGGAGGCT
  CAATACGTCAGTCCCCCTTGAGCTCCTCCTCGCCCTTCTCCAACCGGCCTCCCCTGCCGCCTACCCCCAG
  CAGGGCCTTGGATGACAAACCCCCTCCACCACCTCCTCCAGTGGGCAACAGGCCCTCCATCCACAGGGAA
  GCGGTTCCCCCTCCTCCTCCTCAGAACAACAAGCCTCCAGTGCCTTCCACTCCGCGGCCTTCGGCCTCCT
  CACAGGCCCCACCTCCGCCGCCACCTCCCAGCAGGCCCGGGCCGCCTCCTCTGCCTCCAAGTTCCAGCGG
  CAATGACGAAACCCCAAGACTCCCACAGCGGAATCTGTCCCTCAGTTCGTCCACGCCCCCGTTACCTTCG
  CCAGGACGTTCAGGTCCTCTTCCTCCCCCGCCCAGTGAGAGACCCCCACCTCCAGTGAGGGACCCGCCAG
  GCCGATCAGGCCCCCTCCCACCACCTCCTCCAGTAAGCAGAAACGGCAGCACATCTCGGGCCCTGCCTGC
  TACCCCTCAGTTGCCATCCAGGAGTGGAGTAGACAGTCCCAGGAGTGGACCCAGGCCTCCCCTTCCTCCT
  GATAGGCCCAGTGCTGGGGCACCTCCCCCACCTCCACCATCAACATCTATTAGAAATGGCTTCCAAGACT
  CTCCATGTGAAGATGAGTGGGAAAGCAGATTCTACTTCCATCCGATTTCCGATTTGCCACCTCCAGAGCC
  ATATGTACAAACGACCAAAAGTTATCCCAGCAAACTGGCAAGAAACGAAAGCCGGAGTGGATCCAACCGA
  AGAGAAAGGGGTGCTCCACCACTCCCTCCCATCCCGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217601 representing NM_003387
  Red=Cloning site Green=Tags(s)
  
  MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGG
  GGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKP
  FSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHNRGSPPVPGGP
  RQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHRE
  AVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPS
  PGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPP
  DRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNR
  RERGAPPLPPIPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003387
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003387.5
• RefSeq Size: 4605 bp
• RefSeq ORF: 1512 bp
• Locus ID: 7456
• UniProt ID: O43516
• Cytogenetics: 2q31.1
• Domains: WH2
• MW: 51.1 kDa
• Gene Summary: This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]