Kindlin (FERMT1) (NM_017671) Human Tagged ORF Clone

CAT#: RC217593

FERMT1 (Myc-DDK-tagged)-Human fermitin family member 1 (FERMT1)

Product Images

Circular map for RC217593

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  Western blot validation of overexpression lysate (Cat# LY413620) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217593 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FERMT1 protein (Cat# TP317593). The protein was produced from HEK293T cells transfected with FERMT1 cDNA clone (Cat# RC217593) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kindlin
• Synonyms: C20orf42; DTGCU2; KIND1; UNC112A; URP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217593 representing NM_017671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCATCCACTGACTTTACATTTGCTTCCTGGGAGCTTGTGGTCCGCGTTGACCATCCCAATGAAG
  AGCAGCAGAAAGACGTCACACTGAGAGTATCTGGAGACCTTCATGTTGGAGGAGTGATGCTCAAGTTAGT
  AGAACAGATCAATATATCCCAAGACTGGTCAGACTTTGCTCTTTGGTGGGAACAGAAGCATTGCTGGCTT
  CTGAAAACCCACTGGACCCTGGACAAATATGGGGTCCAGGCAGATGCAAAGCTTCTCTTCACCCCTCAGC
  ATAAAATGCTGCGCCTTCGTCTGCCGAATTTGAAGATGGTGAGGTTGCGAGTCAGCTTCTCAGCTGTGGT
  TTTTAAAGCTGTCAGTGATATCTGCAAAATCCTGAATATTAGAAGATCAGAAGAGCTTTCCTTGTTAAAG
  CCGTCTGGTGACTATTTTAAGAAGAAGAAGAAAAAAGACAAAAATAATAAGGAACCCATAATTGAAGATA
  TTCTAAACCTGGAGAGTTCTCCAACAGCTTCAGGTTCATCAGTAAGTCCTGGTTTATACAGTAAAACCAT
  GACCCCTATATATGACCCCATCAATGGAACACCAGCATCATCCACCATGACTTGGTTCAGTGACAGCCCT
  TTGACGGAACAAAACTGCAGCATCCTCGCATTCAGCCAACCCCCCCAGTCCCCAGAAGCACTTGCGGATA
  TGTACCAGCCTCGGTCTCTGGTTGATAAAGCCAAGCTCAATGCAGGTTGGCTAGACTCCTCACGCTCCCT
  TATGGAACAAGGCATCCAAGAGGATGAGCAGCTGCTCTTACGATTTAAATATTATTCTTTCTTCGACTTG
  AATCCTAAATATGATGCTGTCCGAATAAACCAACTCTATGAGCAAGCCAGGTGGGCCATTCTCTTAGAAG
  AAATTGATTGCACAGAGGAAGAAATGTTGATCTTTGCAGCTCTACAGTACCACATTAGCAAACTGTCGTT
  GTCTGCTGAAACACAGGATTTTGCAGGCGAGTCCGAGGTTGATGAAATAGAAGCGGCGCTTTCTAATTTG
  GAAGTAACCCTAGAAGGTGGAAAAGCGGACAGCCTTTTGGAGGACATTACTGATATCCCTAAACTTGCAG
  ATAATCTCAAATTATTTAGGCCCAAGAAGTTACTACCAAAAGCTTTCAAACAATATTGGTTTATCTTTAA
  AGACACATCCATAGCATACTTTAAAAATAAGGAACTTGAACAAGGAGAACCACTAGAAAAACTAAATCTT
  AGAGGCTGCGAAGTTGTGCCCGATGTAAATGTAGCAGGAAGAAAATTTGGAATCAAGTTACTAATCCCTG
  TTGCCGATGGTATGAATGAAATGTATTTGAGATGTGACCATGAGAATCAATACGCCCAATGGATGGCTGC
  CTGCATGTTGGCATCGAAGGGCAAAACCATGGCAGACAGCTCCTACCAGCCAGAGGTCCTCAACATCCTT
  TCATTTCTGAGGATGAAAAACAGGAACTCTGCATCTCAGGTGGCTTCCAGTCTCGAAAACATGGATATGA
  ACCCAGAATGTTTTGTGTCACCACGGTGTGCAAAAAGACACAAATCCAAACAGCTGGCCGCCCGGATCCT
  GGAGGCGCACCAGAACGTGGCCCAGATGCCCCTGGTCGAAGCCAAGCTGCGGTTCATCCAGGCGTGGCAG
  TCACTGCCTGAGTTTGGCCTCACCTACTACCTTGTCAGATTTAAAGGAAGCAAAAAAGATGACATTCTGG
  GAGTTTCATATAACAGGTTGATTAAAATTGATGCAGCCACCGGGATTCCAGTGACAACATGGAGATTCAC
  AAATATCAAACAGTGGAATGTAAACTGGGAAACCCGGCAGGTGGTCATCGAGTTTGACCAAAACGTCTTT
  ACTGCTTTCACCTGCCTGAGTGCAGATTGCAAGATTGTGCACGAGTACATTGGCGGCTACATTTTCTTGT
  CCACCCGCTCCAAGGACCAGAATGAAACACTCGATGAGGACTTGTTCCACAAATTGACCGGCGGTCAGGA
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217593 representing NM_017671
  Red=Cloning site Green=Tags(s)
  
  MLSSTDFTFASWELVVRVDHPNEEQQKDVTLRVSGDLHVGGVMLKLVEQINISQDWSDFALWWEQKHCWL
  LKTHWTLDKYGVQADAKLLFTPQHKMLRLRLPNLKMVRLRVSFSAVVFKAVSDICKILNIRRSEELSLLK
  PSGDYFKKKKKKDKNNKEPIIEDILNLESSPTASGSSVSPGLYSKTMTPIYDPINGTPASSTMTWFSDSP
  LTEQNCSILAFSQPPQSPEALADMYQPRSLVDKAKLNAGWLDSSRSLMEQGIQEDEQLLLRFKYYSFFDL
  NPKYDAVRINQLYEQARWAILLEEIDCTEEEMLIFAALQYHISKLSLSAETQDFAGESEVDEIEAALSNL
  EVTLEGGKADSLLEDITDIPKLADNLKLFRPKKLLPKAFKQYWFIFKDTSIAYFKNKELEQGEPLEKLNL
  RGCEVVPDVNVAGRKFGIKLLIPVADGMNEMYLRCDHENQYAQWMAACMLASKGKTMADSSYQPEVLNIL
  SFLRMKNRNSASQVASSLENMDMNPECFVSPRCAKRHKSKQLAARILEAHQNVAQMPLVEAKLRFIQAWQ
  SLPEFGLTYYLVRFKGSKKDDILGVSYNRLIKIDAATGIPVTTWRFTNIKQWNVNWETRQVVIEFDQNVF
  TAFTCLSADCKIVHEYIGGYIFLSTRSKDQNETLDEDLFHKLTGGQD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017671
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017671.5
• RefSeq Size: 5168 bp
• RefSeq ORF: 2034 bp
• Locus ID: 55612
• UniProt ID: Q9BQL6
• Cytogenetics: 20p12.3
• MW: 77.3 kDa
• Gene Summary: This gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq, Dec 2009]