JKAMP (NM_001098625) Human Tagged ORF Clone

CAT#: RC217546

JKAMP (Myc-DDK-tagged)-Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 2

Product Images

Circular map for RC217546

• 

  Western blot validation of overexpression lysate (Cat# LY420648) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217546 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JKAMP
• Synonyms: C14orf100; C24orf100; CDA06; HSPC213; HSPC327; JAMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217546 representing NM_001098625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGCCTTGGACTTTATTGTGGGAAGACCCTATTATTTAAAAATGGCTCAACTGAAATATATGGAG
  AATGTGGGGTATGCCCAAGAGGACAGAGAACGAATGCACAGAAATATTGTCAGCCTTGCACAGAATCTCC
  TGAACTTTATGATTGGCTCTATCTTGGATTTATGGCAATGCTTCCTCTGGTTTTACATTGGTTCTTCATT
  GAATGGTACTCGGGGAAAAAGAGTTCCAGCGCACTTTTCCAACACATCACTGCATTATTTGAATGCAGCA
  TGGCAGCTATTATCACCTTACTTGTGAGTGATCCAGTTGGTGTTCTTTATATTCGTTCATGTCGAGTATT
  GATGCTTTCTGACTGGTACACGATGCTTTACAACCCAAGTCCAGATTACGTTACCACAGTACACTGTACT
  CATGAAGCCGTCTACCCACTATATACCATTGTATTTATCTATTACGCATTCTGCTTGGTATTAATGATGC
  TGCTCCGACCTCTTCTGGTGAAGAAGATTGCATGTGGGTTAGGGAAATCTGATCGATTTAAAAGTATTTA
  TGCTGCACTTTACTTCTTCCCAATTTTAACCGTGCTTCAGGCAGTTGGTGGAGGCCTTTTATATTACGCC
  TTCCCATACATTATATTAGTGTTATCTTTGGTTACTCTGGCTGTGTACATGTCTGCTTCTGAAATAGAGA
  ACTGCTATGATCTTCTGGTCAGAAAGAAAAGACTTATTGTTCTCTTCAGCCACTGGTTACTTCATGCCTA
  TGGAATAATCTCCATTTCCAGAGTGGATAAACTTGAGCAAGATTTGCCCCTTTTGGCTTTGGTACCTACA
  CCAGCCCTTTTTTACTTGTTCACTGCAAAATTTACCGAACCTTCAAGGATACTCTCAGAAGGAGCCAATG
  GACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217546 representing NM_001098625
  Red=Cloning site Green=Tags(s)
  
  MACLGLYCGKTLLFKNGSTEIYGECGVCPRGQRTNAQKYCQPCTESPELYDWLYLGFMAMLPLVLHWFFI
  EWYSGKKSSSALFQHITALFECSMAAIITLLVSDPVGVLYIRSCRVLMLSDWYTMLYNPSPDYVTTVHCT
  HEAVYPLYTIVFIYYAFCLVLMMLLRPLLVKKIACGLGKSDRFKSIYAALYFFPILTVLQAVGGGLLYYA
  FPYIILVLSLVTLAVYMSASEIENCYDLLVRKKRLIVLFSHWLLHAYGIISISRVDKLEQDLPLLALVPT
  PALFYLFTAKFTEPSRILSEGANGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098625
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098625.2, NP_001092095.1
• RefSeq Size: 2359 bp
• RefSeq ORF: 918 bp
• Locus ID: 51528
• UniProt ID: Q9P055
• Cytogenetics: 14q23.1
• Protein Families: Transmembrane
• MW: 34.4 kDa
• Gene Summary: May be a regulator of the duration of MAPK8 activity in response to various stress stimuli. Facilitates degradation of misfolded endoplasmic reticulum (ER) luminal proteins through the recruitment of components of the proteasome and endoplasmic reticulum-associated degradation (ERAD) system (By similarity).[UniProtKB/Swiss-Prot Function]