PPIL3 (NM_131916) Human Tagged ORF Clone

CAT#: RC217389

PPIL3 (Myc-DDK-tagged)-Human peptidylprolyl isomerase (cyclophilin)-like 3 (PPIL3), transcript variant PPIL3c

Product Images

Circular map for RC217389

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPIL3 (Cat# RC217389, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPIL3(Cat# TA505349). Positive lysates LY408889 (100ug) and LC408889 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY408889) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217389 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PPIL3 protein (Cat# TP317389). The protein was produced from HEK293T cells transfected with PPIL3 cDNA clone (Cat# RC217389) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPIL3
• Synonyms: cyclophilin-like protein 3; cyclophilin J; CYPJ; peptidylprolyl cis-trans isomerase-like protein 3; peptidylprolyl isomerase (cyclophilin)-like 3; peptidylprolyl isomerase-like 3; PPIase-like protein 3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217389 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGACACTGCATACAGATGTAGGTGATATTAAAATTGAAGTCTTCTGTGAGAGGACACCCAAAA
  CATGTGAGAATTTCTTGGCTCTTTGTGCCAGTAATTACTACAATGGCTGTATATTTCATAGGAATATCAA
  GGGTTTCATGGTTCAAACAGGAGATCCAACAGGAACTGGAAGAGGAGGCAACAGTATTTGGGGCAAGAAG
  TTTGAGGATGAATACAGTGAATATCTTAAGCACAATGTTAGAGGTGTTGTATCTATGGCTAATAATGGCC
  CGAACACCAATGGATCTCAGTTCTTCATCACCTATGGCAAACAGCCACATTTGGACATGAAATACACCGT
  ATTTGGAAAGGTAATAGATGGTCTGGAAACTCTAGATGAGTTGGAGAAGTTGCCAGTAAATGAGAAGACA
  TACCGACCTCTTAATGAGGTACACATTAAGGACATAACTATTCATGCCAACCCATTTGCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217389 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVTLHTDVGDIKIEVFCERTPKTCENFLALCASNYYNGCIFHRNIKGFMVQTGDPTGTGRGGNSIWGKK
  FEDEYSEYLKHNVRGVVSMANNGPNTNGSQFFITYGKQPHLDMKYTVFGKVIDGLETLDELEKLPVNEKT
  YRPLNEVHIKDITIHANPFAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_131916
• ORF Size: 483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_131916.1, NP_572028.1
• RefSeq Size: 1620 bp
• RefSeq ORF: 485 bp
• Locus ID: 53938
• Cytogenetics: 2q33.1
• MW: 18.2 kDa
• Gene Summary: This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2008]