TP53BP2 (NM_001031685) Human Tagged ORF Clone

CAT#: RC217316

TP53BP2 (Myc-DDK-tagged)-Human tumor protein p53 binding protein, 2 (TP53BP2), transcript variant 1

Product Images

Circular map for RC217316

• 

  Western blot validation of overexpression lysate (Cat# LY421900) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217316 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TP53BP2
• Synonyms: 53BP2; ASPP2; BBP; P53BP2; PPP1R13A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217316 representing NM_001031685
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGATGTTTCTTACCGTGTATCTCAGTAACAATGAGCAGCACTTCACAGAAGTTCCAGTTACTCCAG
  AAACAATATGCAGAGACGTGGTGGATCTGTGCAAAGAACCCGGCGAGAGTGATTGCCATTTGGCTGAAGT
  GTGGTGTGGCTCTGAACGTCCAGTTGCGGATAATGAGCGAATGTTTGATGTTCTTCAACGATTTGGAAGT
  CAGAGGAACGAAGTTCGCTTCTTCCTTCGTCATGAACGCCCCCCTGGCAGGGACATTGTGAGTGGACCAA
  GATCTCAGGATCCAAGTTTAAAAAGAAATGGTGTAAAAGTTCCTGGTGAATATCGAAGAAAGGAGAACGG
  TGTTAATAGTCCTAGGATGGATCTGACTCTTGCTGAACTTCAGGAAATGGCATCTCGCCAGCAGCAACAG
  ATTGAAGCCCAGCAACAATTGCTGGCAACTAAGGAACAGCGCTTAAAGTTTTTGAAACAACAAGATCAGC
  GACAACAGCAACAAGTTGCTGAGCAGGAGAAACTTAAAAGGCTAAAAGAAATAGCTGAGAATCAGGAAGC
  TAAGCTAAAAAAAGTGAGAGCACTTAAAGGCCACGTGGAACAGAAGAGACTAAGCAATGGGAAACTTGTG
  GAGGAAATTGAACAGATGAATAATTTGTTCCAGCAAAAACAGAGGGAGCTCGTCCTGGCTGTGTCAAAAG
  TAGAAGAACTGACCAGGCAGCTAGAGATGCTCAAGAACGGCAGGATCGACAGCCACCATGACAATCAGTC
  TGCAGTGGCTGAGCTTGATCGCCTCTATAAGGAGCTGCAGCTAAGAAACAAATTGAATCAAGAGCAGAAT
  GCCAAGCTACAACAACAGAGGGAGTGTTTGAATAAGCGTAATTCAGAAGTGGCAGTCATGGATAAGCGTG
  TTAATGAGCTGAGGGACCGGCTGTGGAAGAAGAAGGCAGCTCTACAGCAAAAAGAAAATCTACCAGTTTC
  ATCTGATGGAAATCTTCCCCAGCAAGCCGCGTCAGCCCCAAGCCGTGTGGCTGCAGTAGGTCCCTATATC
  CAGTCGTCTACTATGCCTCGGATGCCCTCAAGGCCTGAATTGCTGGTGAAGCCAGCCCTGCCGGATGGTT
  CCTTGGTCATTCAGGCTTCAGAGGGGCCGATGAAAATACAGACACTGCCCAACATGAGATCTGGGGCTGC
  TTCACAAACTAAAGGCTCTAAAATCCATCCAGTTGGCCCTGATTGGAGTCCTTCAAATGCAGATCTTTTC
  CCAAGCCAAGGCTCTGCTTCTGTACCTCAAAGCACTGGGAATGCTCTGGATCAAGTTGATGATGGAGAGG
  TTCCGCTGAGGGAGAAAGAGAAGAAAGTGCGTCCGTTCTCAATGTTTGATGCAGTAGACCAGTCCAATGC
  CCCACCTTCCTTTGGTACTCTGAGGAAGAACCAGAGCAGTGAAGATATCTTGCGGGATGCTCAGGTTGCA
  AATAAAAATGTGGCTAAAGTACCACCTCCTGTTCCTACAAAACCAAAACAGATTAATTTGCCTTATTTTG
  GACAAACTAATCAGCCACCTTCAGACATTAAGCCAGACGGAAGTTCTCAGCAGTTGTCAACAGTTGTTCC
  GTCCATGGGAACTAAACCAAAACCAGCAGGGCAGCAGCCGAGAGTGCTGCTATCTCCCAGCATACCTTCG
  GTTGGCCAAGACCAGACCCTTTCTCCAGGTTCTAAGCAAGAAAGTCCACCTGCTGCTGCCGTCCGGCCCT
  TTACTCCCCAGCCTTCCAAAGACACCTTACTTCCACCCTTCAGAAAACCCCAGACCGTGGCAGCAAGTTC
  AATATATTCCATGTATACGCAACAGCAGGCGCCAGGAAAAAACTTCCAGCAGGCTGTGCAGAGCGCGTTG
  ACCAAGACTCATACCAGAGGGCCACACTTTTCAAGTGTATATGGTAAGCCTGTAATTGCTGCTGCCCAGA
  ATCAACAGCAGCACCCAGAGAACATTTATTCCAATAGCCAGGGCAAGCCTGGCAGTCCAGAACCTGAAAC
  AGAGCCTGTTTCTTCAGTTCAGGAGAACCATGAAAACGAAAGAATTCCTCGGCCACTCAGCCCAACTAAA
  TTACTGCCTTTCTTATCTAATCCTTACCGAAACCAGAGTGATGCTGACCTAGAAGCCTTACGAAAGAAAC
  TGTCTAACGCACCAAGGCCTCTAAAGAAACGTAGTTCTATTACAGAGCCAGAGGGTCCTAATGGGCCAAA
  TATTCAGAAGCTTTTATATCAGAGGACCACCATAGCGGCCATGGAGACCATCTCTGTCCCATCATACCCA
  TCCAAGTCAGCTTCTGTGACTGCCAGCTCAGAAAGCCCAGTAGAAATCCAGAATCCATATTTACATGTGG
  AGCCCGAAAAGGAGGTGGTCTCTCTGGTTCCTGAATCATTGTCCCCAGAGGATGTGGGGAATGCCAGTAC
  AGAGAACAGTGACATGCCAGCTCCTTCTCCAGGCCTTGATTATGAGCCTGAGGGAGTCCCAGACAACAGC
  CCAAATCTCCAGAATAACCCAGAAGAACCAAATCCAGAGGCTCCACATGTGCTTGATGTGTACCTGGAGG
  AGTACCCTCCATACCCACCCCCACCATACCCATCTGGGGAGCCTGAAGGGCCCGGAGAAGACTCGGTGAG
  CATGCGCCCGCCTGAAATCACCGGGCAGGTCTCTCTGCCTCCTGGTAAAAGGACAAACTTGCGTAAAACT
  GGCTCAGAGCGTATCGCTCATGGAATGAGGGTGAAATTCAACCCCCTTGCTTTACTGCTAGATTCGTCTT
  TGGAGGGAGAATTTGACCTTGTACAGAGAATTATTTATGAGGTTGATGACCCAAGCCTCCCCAATGATGA
  AGGCATCACGGCTCTTCACAATGCTGTGTGTGCAGGCCACACAGAAATCGTTAAGTTCCTGGTACAGTTT
  GGTGTAAATGTAAATGCTGCTGATAGTGATGGATGGACTCCATTACATTGTGCTGCCTCATGTAACAACG
  TCCAAGTGTGTAAGTTTTTGGTGGAGTCAGGAGCCGCTGTGTTTGCCATGACCTACAGTGACATGCAGAC
  TGCTGCAGATAAGTGCGAGGAAATGGAGGAAGGCTACACTCAGTGCTCCCAATTTCTTTATGGAGTTCAG
  GAGAAGATGGGCATAATGAATAAAGGAGTCATTTATGCGCTTTGGGATTATGAACCTCAGAATGATGATG
  AGCTGCCCATGAAAGAAGGAGACTGCATGACAATCATCCACAGGGAAGACGAAGATGAAATCGAATGGTG
  GTGGGCGCGCCTTAATGATAAGGAGGGATATGTTCCACGTAACTTGCTGGGACTGTACCCAAGAATTAAA
  CCAAGACAAAGGAGCTTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217316 representing NM_001031685
  Red=Cloning site Green=Tags(s)
  
  MPMFLTVYLSNNEQHFTEVPVTPETICRDVVDLCKEPGESDCHLAEVWCGSERPVADNERMFDVLQRFGS
  QRNEVRFFLRHERPPGRDIVSGPRSQDPSLKRNGVKVPGEYRRKENGVNSPRMDLTLAELQEMASRQQQQ
  IEAQQQLLATKEQRLKFLKQQDQRQQQQVAEQEKLKRLKEIAENQEAKLKKVRALKGHVEQKRLSNGKLV
  EEIEQMNNLFQQKQRELVLAVSKVEELTRQLEMLKNGRIDSHHDNQSAVAELDRLYKELQLRNKLNQEQN
  AKLQQQRECLNKRNSEVAVMDKRVNELRDRLWKKKAALQQKENLPVSSDGNLPQQAASAPSRVAAVGPYI
  QSSTMPRMPSRPELLVKPALPDGSLVIQASEGPMKIQTLPNMRSGAASQTKGSKIHPVGPDWSPSNADLF
  PSQGSASVPQSTGNALDQVDDGEVPLREKEKKVRPFSMFDAVDQSNAPPSFGTLRKNQSSEDILRDAQVA
  NKNVAKVPPPVPTKPKQINLPYFGQTNQPPSDIKPDGSSQQLSTVVPSMGTKPKPAGQQPRVLLSPSIPS
  VGQDQTLSPGSKQESPPAAAVRPFTPQPSKDTLLPPFRKPQTVAASSIYSMYTQQQAPGKNFQQAVQSAL
  TKTHTRGPHFSSVYGKPVIAAAQNQQQHPENIYSNSQGKPGSPEPETEPVSSVQENHENERIPRPLSPTK
  LLPFLSNPYRNQSDADLEALRKKLSNAPRPLKKRSSITEPEGPNGPNIQKLLYQRTTIAAMETISVPSYP
  SKSASVTASSESPVEIQNPYLHVEPEKEVVSLVPESLSPEDVGNASTENSDMPAPSPGLDYEPEGVPDNS
  PNLQNNPEEPNPEAPHVLDVYLEEYPPYPPPPYPSGEPEGPGEDSVSMRPPEITGQVSLPPGKRTNLRKT
  GSERIAHGMRVKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQF
  GVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMTYSDMQTAADKCEEMEEGYTQCSQFLYGVQ
  EKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRNLLGLYPRIK
  PRQRSLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031685
• ORF Size: 3381 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031685.1, NP_001026855.1
• RefSeq Size: 4670 bp
• RefSeq ORF: 3405 bp
• Locus ID: 7159
• UniProt ID: Q13625
• Cytogenetics: 1q41
• Protein Families: Druggable Genome
• MW: 126.1 kDa
• Gene Summary: This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]