BDNF (NM_170735) Human Tagged ORF Clone
BDNF (Myc-DDK-tagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 1
|Product Name||BDNF (NM_170735) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC217190 representing NM_170735
Red=Cloning site Blue=ORF Green=Tags(s)
>RC217190 representing NM_170735
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||741 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_170735.1, NM_170735.2, NM_170735.3, NM_170735.4, NM_170735.5, NP_733931.1|
|RefSeq Size||4247 bp|
|RefSeq ORF||744 bp|
|Protein Families||Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane|
|Protein Pathways||Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway|
|Gene Summary||This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
Sustained adrenergic signaling promotes intratumoral innervation through BDNF induction
,Allen, JK;Armaiz-Pena, GN;Nagaraja, AS;Sadaoui, NC;Ortiz, T;Dood, R;Ozcan, M;Herder, DM;Haemerrle, M;Gharpure, KM;Rupaimoole, R;Previs, R;Wu, SY;Pradeep, S;Xu, X;Dong Han, H;Zand, B;Dalton, HJ;Taylor, M;Hu, W;Bottsford-Miller, J;Moreno-Smith, M;Kang, Y;Mangala, LS;Rodriguez-Aguayo, C;Sehgal, V;Spaeth, EL;Ram, PT;Wong, ST;Marini, FC;Lopez-Berestein, G;Cole, SW;Lutgendorf, SK;diBiasi, M;Sood, AK;,
Cancer Res. 2018
,PubMed ID 29661830
MicroRNA‑744 inhibits tumor cell proliferation and invasion of gastric cancer via targeting brain‑derived neurotrophic factor
,Xu, AJ;Fu, LN;Wu, HX;Yao, XL;Meng, R;,
Mol Med Rep 2017
,PubMed ID 28791400
In vitro bioassay model for screening non-viral neurotrophic factor gene delivery systems for glaucoma treatment
,Chen, DW;Foldvari, M;,
Drug Deliv Transl Res 2016
,PubMed ID 27549107
|cDNA Clone Resources|
|SC309453||BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 1||
|RG217190||BDNF (GFP-tagged) - Human brain-derived neurotrophic factor (BDNF), transcript variant 1||
|RC217190L1||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 1, Myc-DDK-tagged||
|RC217190L2||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 1, mGFP tagged||
|RC217190L3||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 1, Myc-DDK-tagged||
|RC217190L4||Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 1, mGFP tagged||