Cadherin 7 (CDH7) (NM_004361) Human Tagged ORF Clone

CAT#: RC217116

CDH7 (Myc-DDK-tagged)-Human cadherin 7, type 2 (CDH7), transcript variant b

Product Images

Circular map for RC217116

• 

  Western blot validation of overexpression lysate (Cat# LY401390) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217116 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cadherin 7
• Synonyms: CDH7L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217116 representing NM_004361
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTGGGCAAAGTGGAGTTCTGCCATTTTCTGCAGCTAATAGCTCTTTTCCTGTGTTTTTCTGGGA
  TGAGTCAAGCAGAACTCTCAAGGTCCAGATCAAAGCCCTATTTCCAATCAGGGAGGTCCCGGACCAAGCG
  CAGCTGGGTGTGGAATCAGTTCTTTGTGCTGGAGGAATACATGGGTTCAGACCCCCTCTATGTAGGAAAG
  CTTCACTCTGATGTTGATAAAGGAGATGGTTCCATCAAATACATCTTGTCAGGCGAAGGGGCAAGTTCCA
  TTTTCATTATTGATGAGAACACTGGGGATATTCATGCCACCAAGAGACTGGATCGTGAGGAGCAGGCCTA
  CTACACGCTCCGAGCTCAAGCGCTGGATAGGCTCACCAACAAACCCGTGGAGCCCGAGTCGGAGTTTGTC
  ATCAAAATTCAGGATATCAACGACAATGAACCCAAATTTTTGGATGGCCCATACACGGCAGGAGTTCCCG
  AAATGTCTCCCGTGGGGACCTCAGTGGTACAAGTGACAGCGACGGATGCTGATGATCCTACATATGGCAA
  CAGTGCCAGAGTGGTCTACAGTATTCTGCAAGGACAGCCGTACTTCTCAGTGGAGCCAAAGACAGGAGTC
  ATCAAGACTGCCCTTCCAAACATGGATAGAGAGGCTAAAGACCAGTATTTGCTTGTTATTCAGGCAAAGG
  ATATGGTTGGTCAAAATGGAGGACTGTCAGGAACTACATCAGTCACTGTGACCCTAACTGATGTCAACGA
  TAATCCACCTCGCTTTCCTCGAAGGTCTTATCAATATAACGTCCCAGAGTCATTACCTGTAGCCTCAGTT
  GTGGCCAGAATTAAAGCTGCTGATGCAGATATTGGAGCTAATGCTGAAATGGAGTACAAGATTGTGGATG
  GTGATGGTTTGGGCATTTTTAAGATTTCTGTTGACAAAGAAACCCAGGAAGGAATCATTACTATACAGAA
  GGAGCTGGATTTTGAAGCCAAAACAAGTTACACGCTACGGATAGAAGCTGCAAATAAAGATGCCGACCCT
  CGCTTTCTGAGCTTGGGTCCGTTCAGTGACACGACAACTGTGAAGATAATTGTGGAAGATGTAGATGAGC
  CCCCTGTGTTCTCTTCACCCTTGTACCCTATGGAGGTGTCGGAAGCTACCCAGGTTGGGAATATCATTGG
  CACTGTAGCAGCTCATGACCCAGATTCTTCCAATAGCCCTGTGAGGTACTCAATTGACAGAAACACAGAC
  TTGGAGAGATACTTCAATATTGATGCCAACAGTGGGGTCATCACAACTGCCAAGTCTTTGGATCGAGAGA
  CAAATGCTATTCACAATATCACAGTCCTTGCAATGGAGAGCCAGAATCCATCTCAAGTAGGAAGAGGCTA
  TGTGGCCATCACTATACTTGACATCAATGATAACGCCCCTGAATTTGCCATGGACTATGAGACCACCGTC
  TGTGAAAATGCCCAGCCGGGGCAGGTTATCCAGAAAATCAGTGCTGTGGATAAAGATGAGCCATCCAATG
  GACACCAGTTTTACTTCAGCTTAACAACGGATGCAACAAATAACCACAACTTTTCATTGAAAGATAACAA
  AGACAACACAGCCTCAATACTGACCAGGAGAAACGGCTTCCGGAGACAGGAACAATCAGTTTACTATCTG
  CCAATTTTCATTGTGGACAGTGGATCTCCCTCACTTAGCAGCACCAGCACCCTCACCATCCGCGTGTGTG
  ACTGTGATGCTGACGGCGTAGCCCAGACCTGCAATGCAGAGGCCTATGTCCTACCTGCTGGCCTCAGTAC
  AGGAGCCCTGATAGCCATACTCGCCTGTGTCTTGACATTATTGGTGTTGATCCTCCTTATCGTCACTATG
  AGAAGACGGAAAAAAGAGCCCCTTATTTTTGACGAAGAAAGAGACATCAGAGAAAATATTGTGAGATATG
  ATGACGAGGGCGGGGGAGAGGAGGACACGGAAGCGTTTGACATGGCTGCACTGAGAAACCTCAACGTCAT
  CCGAGACACCAAGACCCGGAGGGATGTGACTCCAGAAATTCAATTCCTGAGTCGACCAGCTTTTAAAAGC
  ATCCCAGATAATGTCATCTTTAGGGAATTTATTTGGGAAAGATTAAAAGAAGCCGATGTTGATCCTGGTG
  CTCCTCCTTATGACTCCCTGCAGACATATGCTTTTGAAGGAAATGGCTCAGTTGCTGAATCACTCAGCTC
  TTTAGATTCCATCAGCTCAAACTCTGATCAGAACTATGACTACCTAAGTGACTGGGGACCTCGCTTTAAA
  CGACTCGCGGACATGTATGGGACTGGCCAAGAGAGTTTGTACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217116 representing NM_004361
  Red=Cloning site Green=Tags(s)
  
  MKLGKVEFCHFLQLIALFLCFSGMSQAELSRSRSKPYFQSGRSRTKRSWVWNQFFVLEEYMGSDPLYVGK
  LHSDVDKGDGSIKYILSGEGASSIFIIDENTGDIHATKRLDREEQAYYTLRAQALDRLTNKPVEPESEFV
  IKIQDINDNEPKFLDGPYTAGVPEMSPVGTSVVQVTATDADDPTYGNSARVVYSILQGQPYFSVEPKTGV
  IKTALPNMDREAKDQYLLVIQAKDMVGQNGGLSGTTSVTVTLTDVNDNPPRFPRRSYQYNVPESLPVASV
  VARIKAADADIGANAEMEYKIVDGDGLGIFKISVDKETQEGIITIQKELDFEAKTSYTLRIEAANKDADP
  RFLSLGPFSDTTTVKIIVEDVDEPPVFSSPLYPMEVSEATQVGNIIGTVAAHDPDSSNSPVRYSIDRNTD
  LERYFNIDANSGVITTAKSLDRETNAIHNITVLAMESQNPSQVGRGYVAITILDINDNAPEFAMDYETTV
  CENAQPGQVIQKISAVDKDEPSNGHQFYFSLTTDATNNHNFSLKDNKDNTASILTRRNGFRRQEQSVYYL
  PIFIVDSGSPSLSSTSTLTIRVCDCDADGVAQTCNAEAYVLPAGLSTGALIAILACVLTLLVLILLIVTM
  RRRKKEPLIFDEERDIRENIVRYDDEGGGEEDTEAFDMAALRNLNVIRDTKTRRDVTPEIQFLSRPAFKS
  IPDNVIFREFIWERLKEADVDPGAPPYDSLQTYAFEGNGSVAESLSSLDSISSNSDQNYDYLSDWGPRFK
  RLADMYGTGQESLYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004361
• ORF Size: 2355 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004361.5
• RefSeq Size: 2828 bp
• RefSeq ORF: 2358 bp
• Locus ID: 1005
• UniProt ID: Q9ULB5
• Cytogenetics: 18q22.1
• Protein Families: Transmembrane
• MW: 87.06 kDa
• Gene Summary: This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types. Mutations in this gene may be associated with bipolar disease in human patients. This gene is present in a gene cluster on chromosome 18. [provided by RefSeq, May 2016]