SNX25 (NM_031953) Human Tagged ORF Clone

CAT#: RC216930

SNX25 (Myc-DDK-tagged)-Human sorting nexin 25 (SNX25)

Product Images

Circular map for RC216930

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNX25
• Synonyms: MSTP043; SBBI31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216930 representing NM_031953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAAGCTCTGAAAGAAGTGTTCGACTACAGTTATAGAGATTACATTCTGTCCTGGTATGGAAACC
  TCAGCAGAGATGAGGGACAACTTTACCATCTGCTCTTGGAAGACTTTTGGGAAATTGCCAGACAGCTGCA
  CCACAGACTGAGTCACGTGGATGTGGTTAAAGTTGTCTGCAATGATGTTGTGAGGACTTTACTCACTCAT
  TTCTGTGACCTGAAAGCTGCCAATGCCAGACATGAAGAACAGCCAAGACCTTTTGTGTTGCACGCATGCT
  TGAGGAACTCAGATGATGAAGTAAGATTTCTACAAACGTGTTCTCGGGTTCTGGTGTTTTGTCTCCTCCC
  CTCAAAGGATGTGCAGTCTCTCAGCTTACGTATAATGCTTGCAGAAATTCTCACAACAAAAGTCTTGAAG
  CCGGTAGTGGAGTTACTGAGTAATCCAGATTACATTAACCAAATGCTGCTTGCCCAGCTGGCGTACAGAG
  AGCAAATGAATGAGCATCACAAGAGAGCCTACACCTATGCCCCCTCTTACGAGGACTTCATCAAGCTCAT
  TAACAGCAACTCTGATGTGGAGTTCTTGAAGCAACTAAGGTATCAAATTGTAGTGGAAATAATCCAGGCG
  ACTACAATTAGCAGCTTTCCCCAACTGAAGAGGCACAAAGGTAAAGAAACTGCGGCAATGAAAGCTGATC
  TCCTGAGGGCCAGGAACATGAAGAGGTACATCAACCAACTGACTGTGGCAAAGAAGCAGTGTGAGAAGAG
  AATCCGAATCCTGGGAGGCCCTGCCTATGACCAGCAAGAGGATGGGGCCCTGGATGAGGGGGAAGGGCCT
  CAAAGCCAGAAGATTCTTCAGTTTGAAGATATCTTGGCCAATACGTTCTACCGAGAGCACTTTGGAATGT
  ACATGGAAAGGATGGACAAAAGAGCTCTGATTAGTTTTTGGGAATCTGTGGAACATTTAAAGAATGCTAA
  CAAGAATGAAATTCCACAATTAGTTGGTGAAATTTATCAGAATTTCTTTGTGGAGAGCAAAGAAATATCT
  GTGGAAAAATCACTTTACAAAGAAATTCAGCAGTGTCTTGTAGGAAATAAAGGTATTGAAGTATTCTACA
  AAATCCAGGAAGATGTTTATGAGACCCTAAAGGATAGGTATTACCCTTCATTTATTGTCAGTGACCTGTA
  TGAGAAATTGTTGATAAAAGAGGAAGAAAAACATGCCTCACAGATGATTTCCAACAAGGATGAGATGGGC
  CCAAGAGATGAGGCTGGTGAGGAAGCCGTGGATGATGGTACCAATCAGATCAATGAACAAGCCAGTTTTG
  CTGTAAACAAACTGCGAGAACTAAATGAGAAACTTGAATATAAAAGGCAAGCTCTAAATTCTATTCAAAA
  TGCACCAAAACCTGACAAGAAGATTGTTTCCAAGTTGAAGGATGAAATAATCCTAATAGAGAAAGAACGC
  ACAGACCTTCAGCTGCACATGGCAAGAACGGATTGGTGGTGTGAAAACCTTGGCATGTGGAAAGCCTCCA
  TCACCAGTGGAGAGGTTACAGAAGAGAATGGTGAGCAATTGCCATGTTACTTTGTCATGGTAAGCCTACA
  AGAAGTTGGAGGAGTTGAAACTAAGAACTGGACGGTCCCCAGAAGGCTCAGCGAGTTTCAGAATTTACAC
  CGGAAACTCAGTGAGTGCGTCCCTTCTTTAAAAAAAGTCCAGTTGCCTTCTCTTAGCAAGCTGCCTTTCA
  AATCTATAGATCAAAAGTTTATGGAAAAGTCGAAGAATCAATTAAATAAGTTTTTACAGAATCTGCTTTC
  AGATGAAAGACTGTGTCAGAGTGAAGCACTTTATGCCTTCTTGAGCCCTTCTCCTGACTACCTCAAGGTT
  ATCGACGTGCAGGGGAAAAAAAATTCTTTTTCATTATCCTCATTTTTGGAAAGACTTCCTCGCGACTTCT
  TCTCCCACCAGGAGGAGGAGACAGAGGAGGACAGTGACCTGTCAGATTATGGTGATGATGTGGATGGGAG
  GAAAGACGCCTTGGCTGAACCATGTTTCATGTTGATTGGGGAGATTTTTGAACTTCGAGGAATGTTTAAA
  TGGGTGAGAAGAACATTAATTGCCCTCGTTCAGGTCACTTTTGGAAGAACCATCAACAAACAAATCCGGG
  ACACAGTCAGCTGGATTTTCAGTGAGCAAATGTTGGTTTACTACATCAATATTTTCCGGGATGCTTTTTG
  GCCAAATGGGAAGTTGGCACCACCGACCACAATCAGAAGCAAAGAGCAAAGTCAGGAAACAAAACAGAGA
  GCACAGCAAAAGCTGCTTGAAAACATTCCAGATATGCTTCAGAGCCTTGTTGGACAGCAAAATGCCCGCC
  ACGGTATAATAAAAATATTCAATGCACTGCAAGAAACAAGAGCCAACAAGCATCTGTTATATGCGCTGAT
  GGAACTGCTGCTAATTGAACTGTGTCCTGAGCTGAGAGTTCATTTAGATCAACTTAAAGCTGGCCAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216930 representing NM_031953
  Red=Cloning site Green=Tags(s)
  
  MDKALKEVFDYSYRDYILSWYGNLSRDEGQLYHLLLEDFWEIARQLHHRLSHVDVVKVVCNDVVRTLLTH
  FCDLKAANARHEEQPRPFVLHACLRNSDDEVRFLQTCSRVLVFCLLPSKDVQSLSLRIMLAEILTTKVLK
  PVVELLSNPDYINQMLLAQLAYREQMNEHHKRAYTYAPSYEDFIKLINSNSDVEFLKQLRYQIVVEIIQA
  TTISSFPQLKRHKGKETAAMKADLLRARNMKRYINQLTVAKKQCEKRIRILGGPAYDQQEDGALDEGEGP
  QSQKILQFEDILANTFYREHFGMYMERMDKRALISFWESVEHLKNANKNEIPQLVGEIYQNFFVESKEIS
  VEKSLYKEIQQCLVGNKGIEVFYKIQEDVYETLKDRYYPSFIVSDLYEKLLIKEEEKHASQMISNKDEMG
  PRDEAGEEAVDDGTNQINEQASFAVNKLRELNEKLEYKRQALNSIQNAPKPDKKIVSKLKDEIILIEKER
  TDLQLHMARTDWWCENLGMWKASITSGEVTEENGEQLPCYFVMVSLQEVGGVETKNWTVPRRLSEFQNLH
  RKLSECVPSLKKVQLPSLSKLPFKSIDQKFMEKSKNQLNKFLQNLLSDERLCQSEALYAFLSPSPDYLKV
  IDVQGKKNSFSLSSFLERLPRDFFSHQEEETEEDSDLSDYGDDVDGRKDALAEPCFMLIGEIFELRGMFK
  WVRRTLIALVQVTFGRTINKQIRDTVSWIFSEQMLVYYINIFRDAFWPNGKLAPPTTIRSKEQSQETKQR
  AQQKLLENIPDMLQSLVGQQNARHGIIKIFNALQETRANKHLLYALMELLLIELCPELRVHLDQLKAGQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031953
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031953.2, NP_114159.2
• RefSeq Size: 3237 bp
• RefSeq ORF: 2523 bp
• Locus ID: 83891
• Cytogenetics: 4q35.1
• Domains: RGS, PX
• MW: 97.8 kDa
• Gene Summary: May be involved in several stages of intracellular trafficking.[UniProtKB/Swiss-Prot Function]