LIPT1 (NM_145199) Human Tagged ORF Clone

CAT#: RC216793

LIPT1 (Myc-DDK-tagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5

Product Images

Circular map for RC216793

• 

  Western blot validation of overexpression lysate (Cat# LY408046) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218958 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPT1
• Synonyms: LIPT1D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216793 representing NM_145199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGATCCCATTTTCAATGAAGAATTGCTTCCAGTTACTTTGTAACTGCCAGGTCCCAGCAGCTGGCT
  TTAAAAAAACAGTAAAAAATGGGCTCATTTTACAGTCAATTTCCAATGATGTCTATCAAAATCTGGCTGT
  GGAAGACTGGATCCATGACCATATGAATCTAGAAGGCAAACCAATTCTATTCTTTTGGCAGAATTCTCCC
  TCTGTTGTAATTGGTAGGCATCAAAATCCTTGGCAGGAATGTAACCTGAATCTAATGAGAGAAGAAGGTA
  TAAAACTGGCTCGGAGAAGAAGTGGAGGAGGAACAGTCTACCATGATATGGGTAATATCAATTTGACTTT
  CTTTACAACCAAAAAAAAGTATGATAGAATGGAAAATCTGAAATTAATTGTGAGAGCTCTGAATGCTGTC
  CAACCCCAGCTGGATGTGCAGGCTACCAAAAGATTTGACCTTTTACTTGATGGACAGTTTAAAATCTCAG
  GAACAGCTTCTAAGATCGGCCGGACTACTGCCTATCACCATTGCACTTTATTATGTAGTACTGATGGGAC
  GTTCTTGTCTTCTTTGCTAAAGAGCCCTTACCAAGGGATCAGGAGCAATGCCACTGCTAGCATACCTTCC
  TTAGTGAAAAATCTTTTGGAAAAGGATCCCACTCTGACCTGTGAAGTACTAATGAATGCTGTTGCTACAG
  AGTATGCTGCTTATCATCAAATTGATAATCACATTCACCTAATAAACCCAACGGATGAGACACTGTTTCC
  TGGAATAAATAGCAAAGCCAAAGAACTGCAAACTTGGGAGTGGATATATGGCAAAACTCCAAAGTTTAGT
  ATAAATACTTCCTTTCATGTGTTATATGAACAGTCACACTTGGAAATTAAAGTATTCATAGACATAAAGA
  ATGGAAGAATTGAAATTTGTAATATTGAAGCACCTGATCATTGGTTGCCATTGGAAATACGTGACAAATT
  AAATTCAAGTCTTATTGGCAGTAAGTTTTGCCCAACTGAAACTACCATGCTAACAAATATATTACTTAGA
  ACATGTCCACAAGACCACAAACTAAACAGTAAATGGAATATTCTCTGTGAAAAAATTAAGGGAATAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216793 representing NM_145199
  Red=Cloning site Green=Tags(s)
  
  MLIPFSMKNCFQLLCNCQVPAAGFKKTVKNGLILQSISNDVYQNLAVEDWIHDHMNLEGKPILFFWQNSP
  SVVIGRHQNPWQECNLNLMREEGIKLARRRSGGGTVYHDMGNINLTFFTTKKKYDRMENLKLIVRALNAV
  QPQLDVQATKRFDLLLDGQFKISGTASKIGRTTAYHHCTLLCSTDGTFLSSLLKSPYQGIRSNATASIPS
  LVKNLLEKDPTLTCEVLMNAVATEYAAYHQIDNHIHLINPTDETLFPGINSKAKELQTWEWIYGKTPKFS
  INTSFHVLYEQSHLEIKVFIDIKNGRIEICNIEAPDHWLPLEIRDKLNSSLIGSKFCPTETTMLTNILLR
  TCPQDHKLNSKWNILCEKIKGIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145199
• ORF Size: 1119 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145199.3
• RefSeq Size: 1288 bp
• RefSeq ORF: 1122 bp
• Locus ID: 51601
• UniProt ID: Q9Y234
• Cytogenetics: 2q11.2
• Domains: BPL_LipA_LipB
• Protein Pathways: Lipoic acid metabolism, Metabolic pathways
• MW: 42.3 kDa
• Gene Summary: The process of transferring lipoic acid to proteins is a two-step process. The first step is the activation of lipoic acid by lipoate-activating enzyme to form lipoyl-AMP. For the second step, the protein encoded by this gene transfers the lipoyl moiety to apoproteins. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 13. Read-through transcription also exists between this gene and the neighboring downstream mitochondrial ribosomal protein L30 (MRPL30) gene. [provided by RefSeq, Mar 2011]