EPB41 (NM_203343) Human Tagged ORF Clone

CAT#: RC216689

EPB41 (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) (EPB41), transcript variant 2

Product Images

Circular map for RC216689

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPB41
• Synonyms: 4.1R; EL1; HE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216689 representing NM_203343
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACAGAGAAGAGTTTAGTGACTGAGGCCGAAAATTCACAGCACCAACAGAAGGAAGAGGGTGAGG
  AAGCCATAAACTCAGGCCAACAAGAACCTCAGCAGGAGGAATCTTGTCAAACAGCAGCTGAAGGAGATAA
  TTGGTGTGAACAGAAGCTGAAAGCTTCTAATGGAGACACTCCTACACATGAAGACTTGACCAAGAACAAG
  GAGCGGACATCAGAAAGCAGAGGACTTTCACGACTATTCTCCTCGTTTCTCAAAAGGCCCAAATCTCAGG
  TGTCCGAGGAAGAAGGCAAAGAAGTAGAGTCAGATAAAGAAAAAGGTGAAGGAGGTCAGAAAGAGATAGA
  ATTTGGAACCAGTCTTGATGAAGAGATCATTTTAAAGGCCCCAATTGCAGCTCCTGAACCGGAACTCAAA
  ACAGACCCATCTTTGGATCTTCATTCATTAAGCAGTGCAGAAACACAGCCTGCTCAGGAAGAACTCAGAG
  AAGATCCAGATTTTGAAATTAAGGAAGGAGAAGGACTTGAAGAGTGCTCCAAAATAGAAGTAAAAGAAGA
  AAGCCCTCAATCAAAAGCAGAAACAGAATTAAAAGCTTCCCAAAAACCAATCAGAAAACACAGGAACATG
  CACTGCAAGGTTTCTTTGTTGGATGACACAGTTTATGAATGTGTTGTGGAGACATGGCTGGATTCCGCCA
  AAGAAATAAAAAAGCAGGTTCGTGGTGTCCCTTGGAATTTTACATTTAATGTAAAGTTTTATCCACCTGA
  CCCAGCACAGTTAACAGAAGACATAACAAGATATTATTTATGTCTTCAGCTTCGGCAGGACATAGTTGCA
  GGACGTCTGCCCTGTTCCTTTGCAACCTTAGCATTATTAGGTTCTTACACCATCCAGTCTGAACTGGGAG
  ACTACGACCCAGAACTCCATGGCGTGGATTATGTTAGTGATTTTAAACTGGCCCCGAATCAGACCAAGGA
  ACTTGAAGAGAAGGTCATGGAACTGCATAAGTCATACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAG
  TTTCTTGAGAATGCCAAAAAGTTGTCTATGTATGGAGTTGATCTTCATAAAGCAAAGGACTTGGAAGGAG
  TAGATATCATCCTAGGTGTCTGCTCTAGTGGCCTTCTGGTTTACAAAGATAAGCTGAGAATTAACCGCTT
  CCCTTGGCCCAAAGTGCTGAAGATTTCTTATAAACGTAGTAGCTTTTTCATCAAGATTCGGCCTGGAGAG
  CAAGAGCAGTATGAAAGTACCATCGGATTCAAACTTCCCAGTTACCGAGCAGCTAAGAAATTATGGAAAG
  TCTGTGTAGAACATCACACGTTTTTCAGATTGACATCTACAGACACCATTCCCAAAAGCAAATTTCTTGC
  GCTAGGATCCAAATTTCGATACAGTGGCCGGACTCAAGCTCAGACCAGGCAAGCTAGTGCTCTAATTGAC
  AGGCCTGCCCCACACTTCGAGCGTACAGCAAGTAAACGGGCGTCCCGGAGCCTCGATGGAGCAGCAGCTG
  TCGATTCGGCAGACCGAAGTCCTCGGCCCACTTCTGCACCTGCCATTACTCAGGGTCAGGTTGCAGAAGG
  TGGCGTCCTAGATGCCTCTGCTAAAAAAACAGTGGTCCCTAAAGCACAGAAGGAAACAGTGAAGGCTGAA
  GTGAAAAAGGAAGACGAGCCACCTGAGCAAGCTGAGCCAGAGCCCACAGAAGCATGGAAGGATTTAGACA
  AGAGTCAAGAGGAGATCAAAAAACATCATGCCAGCATCAGTGAGCTGAAAAAGAACTTCATGGAGTCTGT
  ACCAGAACCACGGCCTAGTGAATGGGATAAACGCTTATCCACTCACTCACCCTTCCGAACTCTTAACATC
  AATGGGCAAATCCCCACAGGAGAAGGACCTCCCCTGGTGAAGACACAAACTGTCACCATCTCAGATAATG
  CCAATGCTGTGAAAAGTGAAATCCCAACCAAAGACGTCCCTATTGTCCACACTGAGACCAAGACCATCAC
  TTATGAGGCTGCCCAGACTGACGACAACAGTGGAGACTTGGACCCAGGAGTCTTGCTGACAGCTCAAACT
  ATCACATCTGAGACCCCAAGCAGCACCACCACAACTCAAATTACCAAGACTGTAAAAGGTGGGATTTCAG
  AGACACGTATTGAAAAGAGAATTGTGATCACAGGAGATGCTGATATTGACCATGATCAGGTCCTTGTACA
  AGCCATCAAGGAGGCAAAGGAGCAGCACCCAGACATGTCAGTGACCAAGGTGGTCGTCCACCAGGAGACC
  GAGATTGCTGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216689 representing NM_203343
  Red=Cloning site Green=Tags(s)
  
  MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNK
  ERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELK
  TDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNM
  HCKVSLLDDTVYECVVETWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVA
  GRLPCSFATLALLGSYTIQSELGDYDPELHGVDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLE
  FLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGE
  QEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLTSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALID
  RPAPHFERTASKRASRSLDGAAAVDSADRSPRPTSAPAITQGQVAEGGVLDASAKKTVVPKAQKETVKAE
  VKKEDEPPEQAEPEPTEAWKDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTLNI
  NGQIPTGEGPPLVKTQTVTISDNANAVKSEIPTKDVPIVHTETKTITYEAAQTDDNSGDLDPGVLLTAQT
  ITSETPSSTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQVLVQAIKEAKEQHPDMSVTKVVVHQET
  EIADE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203343
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203343.3
• RefSeq Size: 5694 bp
• RefSeq ORF: 2328 bp
• Locus ID: 2035
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 86.6 kDa
• Gene Summary: The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]