SNAP25 Interacting Protein (SRCIN1) (NM_025248) Human Tagged ORF Clone

CAT#: RC216673

SRCIN1 (Myc-DDK-tagged)-Human SRC kinase signaling inhibitor 1 (SRCIN1)

Product Images

Circular map for RC216673

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNAP25 Interacting Protein
• Synonyms: P140; SNIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216673 representing NM_025248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACGCTCCGTCCCAAGATCCGGAGCGGAGCAGCCCCCCCATGCTGTCTGCGGACGATGCGGAGT
  ACCCGCGGGAGTACCGGACCCTGGGGGGCGGGGGCGGCGGGGGCAGCGGGGGCCGGCGCTTCTCCAACGT
  GGGGCTGGTGCACACGTCCGAGCGGCGGCACACGGTGATCGCGGCCCAGAGTCTGGAGGCGCTCAGCGGG
  CTCCAGAAGGCGGACGCCGACCGCAAGCGTGATGCCTTCATGGACCACCTGAAGAGCAAGTACCCACAGC
  ACGCCCTGGCCCTGCGAGGCCAGCAGGACAGGATGCGAGAGCAGCCAAACTACTGGAGTTTCAAGACCCG
  CAGCTCACGCCACACTCAGGGAGCCCAGCCCGGGCTGGCAGACCAGGCGGCAAAGCTGTCCTACGCCTCC
  GCCGAGTCGCTGGAGACCATGTCGGAGGCCGAGCTGCCCCTGGGCTTCAGCAGGATGAACCGCTTCCGAC
  AGAGCCTGCCTCTCTCCCGCTCGGCCAGCCAGACCAAGCCGCGCTCCCCAGGGGTGCTGTTCCTGCAGTT
  CGGGGAGGAGACTCGGCGCGTGCACATCACGCACGAGGTCAGCAGCCTGGACACGCTGCACGCACTCATC
  GCGCACATGTTCCCGCAGAAGCTCACCATGGGCATGCTTAAGTCGCCCAATACCGCCATCCTCATCAAAG
  ACGAGGCTCGCAACGTCTTCTACGAGCTGGAGGACGTCCGGGACATCCAGGACCGCAGTATTATCAAGAT
  CTACAGAAAGGAGCCCCTCTACGCTGCCTTCCCTGGCTCACATCTCACCAACGGGGACCTCCGGAGAGAG
  ATGGTGTACGCATCGCGGGAGTCCTCGCCCACGCGGCGCCTCAACAACCTGTCACCAGCGCCGCACCTGG
  CATCCGGCTCGCCGCCGCCCGGGCTGCCGTCGGGGCTGCCGTCCGGGCTGCAGTCCGGTTCGCCGTCGCG
  TTCGCGCCTATCGTACGCCGGGGGGCGCCCGCCTTCGTACGCCGGCAGCCCGGTGCACCACGCGGCCGAG
  AGGCTGGGAGGCGCCCCGGCCGCCCAGGGCGTCAGCCCCAGCCCCAGCGCCATCCTGGAGCGGCGCGACG
  TGAAGCCGGACGAGGACCTGGCGAGCAAGGCGGGCGGCATGGTGCTGGTGAAAGGCGAGGGCCTCTATGC
  TGACCCCTACGGGCTGCTGCACGAGGGCCGTCTGAGCCTGGCCGCGGCCGCCGGCGACCCGTTCGCCTAC
  CCGGGCGCCGGCGGCCTCTACAAGCGCGGCTCGGTGCGCTCGCTCAGCACCTACTCGGCCGCCGCGCTGC
  AGTCCGATCTGGAGGACTCCCTGTACAAGGCGGCGGGCGGCGGCGGCCCGCTGTACGGCGACGGCTACGG
  CTTCCGCCTGCCGCCTTCGTCACCGCAGAAGCTGGCCGACGTGGCAGCACCCCCCGGAGGTCCCCCGCCA
  CCGCACAGCCCCTACTCGGGGCCGCCCAGCCGCGGCTCGCCAGTGCGCCAGTCCTTCCGCAAGGACTCGG
  GCTCCTCGTCCGTCTTTGCCGAGAGTCCTGGAGGGAAGACCCGCAGCGCGGGGAGCGCCTCGACGGCCGG
  AGCTCCCCCTTCGGAGCTCTTCCCTGGGCCTGGGGAACGCTCGCTGGTTGGGTTCGGGCCGCCAGTGCCA
  GCCAAGGACACGGAGACCAGGGAGCGCATGGAGGCCATGGAGAAGCAGATTGCCAGCCTCACAGGCCTGG
  TGCAGAGCGCCTTACTGCGAGGCTCTGAGCCTGAGACCCCCAGCGAGAAGATTGAAGGCTCCAATGGAGC
  AGCCACCCCCTCAGCACCCTGTGGGTCAGGCGGCCGGAGCAGCGGGGCCACCCCGGTGTCCGGCCCGCCC
  CCGCCCTCGGCCAGCAGCACCCCCGCAGGTCAGCCTACCGCCGTTAGCCGGCTGCAGATGCAGCTTCACC
  TGCGAGGCCTGCAGAACAGCGCCAGTGACTTGCGCGGCCAGCTCCAGCAGTTGCGCAAGCTCCAGCTACA
  GAACCAGGAGTCGGTGCGCGCGCTGCTGAAGCGCACGGAGGCAGAGCTGAGCATGCGCGTGTCGGAGGCG
  GCGCGGCGGCAGGAGGACCCGCTGCAGCGGCAGCGCACCCTGGTGGAAGAGGAACGGCTGCGCTATCTCA
  ACGACGAGGAGCTTATTACCCAGCAGCTCAATGACCTGGAGAAATCGGTGGAGAAGATCCAGAGAGACGT
  GTCCCACAACCACCGGCTGGTGCCCGGCCCTGAGCTGGAGGAGAAGGCACTGGTGCTGAAGCAGCTCGGG
  GAGACGCTGACAGAGCTCAAGGCTCACTTCCCGGGCCTGCAGAGCAAGATGCGGGTGGTGCTGCGCGTGG
  AGGTGGAGGCGGTGAAGTTCCTGAAGGAGGAGCCCCAGCGCCTGGATGGGCTCCTCAAGCGCTGCCGCGG
  GGTCACGGACACGCTGGCCCAGATCCGAAGGCAAGTGGATGAGGGTGTGTGGCCACCCCCCAACAATCTC
  CTGAGTCAGTCCCCCAAGAAGGTGACGGCAGAGACTGACTTCAACAAGAGCGTGGACTTCGAAATGCCAC
  CCCCCAGCCCCCCGCTGAACCTGCATGAGCTGAGCGGGCCAGCTGAAGGAGCCTCTCTTACCCCCAAGGG
  GGGCAACCCCACCAAAGGCCTGGACACTCCTGGCAAGAGAAGCGTGGACAAAGCTGTGTCTGTTGAGGCT
  GCAGAGCGAGACTGGGAGGAGAAGCGGGCAGCCCTGACCCAGTACAGTGCCAAGGACATCAACCGGCTGC
  TGGAAGAGACACAGGCAGAGCTGCTCAAGGCCATCCCTGACCTGGACTGTGCCAGCAAGGCCCATCCAGG
  CCCGGCCCCCACTCCAGATCACAAGCCCCCCAAGGCCCCCCACGGCCAGAAGGCAGCCCCCCGAACGGAG
  CCCAGTGGGAGGAGGGGCTCAGATGAGTTGACCGTGCCCCGATACCGCACAGAGAAGCCCTCCAAGTCGC
  CCCCACCGCCCCCTCCCCGCCGGAGCTTCCCCTCCTCCCATGGCCTGACCACCACACGTACCGGAGAGGT
  GGTGGTCACCAGCAAGAAGGACTCGGCCTTCATCAAGAAGGCTGAGTCCGAGGAGCTGGAGGTGCAGAAG
  CCCCAGGTGAAGCTGCGCCGGGCTGTGTCTGAGGTGGCCCGCCCAGCCTCCACACCACCCATCATGGCCT
  CGGCCATCAAGGACGAGGATGACGAGGATCGCATCATCGCAGAGCTAGAGAGTGGCGGAGGCAGTGTACC
  ACCCATGAAGGTGGTGACTCCGGGGGCCTCTCGGCTGAAGGCGGCCCAGGGCCAGGCGGGCAGCCCCGAC
  AAAAGCAAACATGGCAAGCAGAGGGCCGAGTACATGCGGATCCAGGCCCAGCAGCAGGCCACTAAACCAT
  CTAAAGAGATGAGCGGGTCGAATGAGACCTCGAGCCCAGTCTCAGAAAAGCCCTCGGCTTCCAGAACCTC
  TATCCCTGTATTGACTTCCTTTGGGGCAAGGAATTCTTCCATCTCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216673 representing NM_025248
  Red=Cloning site Green=Tags(s)
  
  MGNAPSQDPERSSPPMLSADDAEYPREYRTLGGGGGGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSG
  LQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYAS
  AESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKPRSPGVLFLQFGEETRRVHITHEVSSLDTLHALI
  AHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRRE
  MVYASRESSPTRRLNNLSPAPHLASGSPPPGLPSGLPSGLQSGSPSRSRLSYAGGRPPSYAGSPVHHAAE
  RLGGAPAAQGVSPSPSAILERRDVKPDEDLASKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAGDPFAY
  PGAGGLYKRGSVRSLSTYSAAALQSDLEDSLYKAAGGGGPLYGDGYGFRLPPSSPQKLADVAAPPGGPPP
  PHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKTRSAGSASTAGAPPSELFPGPGERSLVGFGPPVP
  AKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKIEGSNGAATPSAPCGSGGRSSGATPVSGPP
  PPSASSTPAGQPTAVSRLQMQLHLRGLQNSASDLRGQLQQLRKLQLQNQESVRALLKRTEAELSMRVSEA
  ARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVSHNHRLVPGPELEEKALVLKQLG
  ETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRGVTDTLAQIRRQVDEGVWPPPNNL
  LSQSPKKVTAETDFNKSVDFEMPPPSPPLNLHELSGPAEGASLTPKGGNPTKGLDTPGKRSVDKAVSVEA
  AERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKAHPGPAPTPDHKPPKAPHGQKAAPRTE
  PSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSAFIKKAESEELEVQK
  PQVKLRRAVSEVARPASTPPIMASAIKDEDDEDRIIAELESGGGSVPPMKVVTPGASRLKAAQGQAGSPD
  KSKHGKQRAEYMRIQAQQQATKPSKEMSGSNETSSPVSEKPSASRTSIPVLTSFGARNSSISF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025248
• ORF Size: 3549 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025248.1, NP_079524.1
• RefSeq Size: 4127 bp
• RefSeq ORF: 3552 bp
• Locus ID: 80725
• UniProt ID: Q9C0H9
• Cytogenetics: 17q12
• MW: 126.9 kDa
• Gene Summary: Acts as a negative regulator of SRC by activating CSK which inhibits SRC activity and downstream signaling, leading to impaired cell spreading and migration. Regulates dendritic spine morphology. Involved in calcium-dependent exocytosis. May play a role in neurotransmitter release or synapse maintenance.[UniProtKB/Swiss-Prot Function]