XRRA1 (NM_182969) Human Tagged ORF Clone

CAT#: RC216596

XRRA1 (Myc-DDK-tagged)-Human X-ray radiation resistance associated 1 (XRRA1)

Product Images

Circular map for RC216596

• 

  Western blot validation of overexpression lysate (Cat# LY405284) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216596 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XRRA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216596 representing NM_182969
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCTCAGGAATCTACAAGCTGGATGATGGGAAGCCTTACCTGAACAACTGCTTCCCAGCCAGAA
  ATCTGCTTCGCGTGCCGGAGGAAGGCCAAGGACACTGGTTAGTGGTTCAGAAAGGTAACCTCAAGAAGAA
  GCCCAAGGGTTTGGTTGGAGCACAAGCTGAACGTCGGGAAAGCCTGAAGGCGACTTCTTTTGAGTTCAAG
  GGGAAGAAAGAGTCTCGGCGGGAAAATCAGGTGGACCTTCCTGGACATATCCTGGACCAGGCTTTTCTGC
  TGAAGCACCACTGTGTGAGGAAGCCATCAGATCTGTGCACCATTAATGCCAAGGAAAATGACTTCAAGCA
  TTTTCATTCTGTGATTTATATCAATGCCTCAGAAAACCTGCTGCCTCTAGAGGCATTTCACACGTTTCCA
  GCCCTAAAGGAACTGGATCTCGCATTTAATGGCATCAAAACTATCTACGTGAAATATGGAGACTTTAAGT
  TATTAGAATTCTTGGACCTTTCCTTCAACAGCCTGACTGTGGAGGCCATCTGTGATTTGGGGATTCTGCC
  ACACCTCCGTGTCCTGCTCCTCACAGGCAATGGCCTTACCTCCCTGCCGCCCAATTTGGCCGTCGCAGAA
  CAGGAGGCATCTGTAACATCGCTGACAAGCAAGAGGTACATCCTGAGGTTCCCAGCGCTGGAGACACTGA
  TGCTGGATGACAACAGACTCTCCAACCCCAGTTGCTTTGCCAGCCTGGCTGGGCTCAGGAGACTGAAGAA
  GTTAAGCTTGGATGAAAACAGGATTATCAGGATCCCATACCTACAGCAAGTTCAGCTCTATGACGAGTCA
  GTAGACTGGAATGGAGGCAGGGGAAGTCCCCATAAAGAGCCCCAATTCATGCTGCAGTCCAAGCCAAGGA
  TGCTTGAGGACTCAGATGAGCAACTGGATTATACTGTACTGCCCATGAAAAAGGATGTTGACCGGACAGA
  GGTTGTGTTTTCATCTTACCCTGGATTTTCAACCTCAGAGACAACCAAGATATGTTCACTTCCTCCCATA
  TTCGAGATCCTTCCTGTGAAGTCACTGAAGGCCAGGAACCAGACGCTGGCCCCACCCTTCCCAGAGCTGA
  GATACCTTAGCCTGGCCTACAACAAGATCGCAAAAGAGGATGCTGTCCTACCAGTAGCTCTCTTCCCATC
  TCTCTGCGAGTTCGTCTTTCATAACAACCCTCTGGTGGCCCATACACGAGGGGTCCCTCCACTGCTGAAG
  AGCTTCCTCCAGGAGCGACTGGGAATCCACTTAATTCGAAGGAAGATAGTAAAGCCTAAGCATCATGTTT
  TGATGTCTCGGAAGGAATCATGGAAGGTGAAAAGCGAAATCCCAAAGGTGCCGAAGCAGCCTCTGGTGCT
  CCATCACCCGCGCATGACGACAACCAAGTCTCCCTCAAAGGATATGCTAGAGCCAGAGGCAGAGCTGGCT
  GAAGATCTGCCCACTACCAAAAGCACTTCTGTGGAGTCAGAGATGCCCACTGAGAACCTGGAAGGCCATT
  CCCCGTCTTGCCGGACCTTCGTGCCACTGCCTCCCATCTGCTCCAACTCCACTGTGCATAGTGAAGAGAC
  CCTGTCCCACCTGAGTGACACAACTGTCCGCCTCAGCCCAGAGCGCCCATCAGATGAGGACTCCAAGAGC
  ACAGAGTCCATCTTCCTGACCCAGGTGAGTGAACTGCCTTCCTCCGTCATCCATAAGGATGATTTAGAGT
  TAAAGGAGAAAGACCAAAAGAAACCACCAACGGCACCCAGAGAGGTGAAAGGGACTCGGAGGAAACTCCC
  AACTGCCTTCCTTCCCAGCAAGTACCACGGCTATGAAGAACTGCTGACAGCCAAGCCTGATCCAGCCTTC
  ATTGAGCCAAAGGGAATCCAGAAGAATGCACAAGCCCTGCAACAAATGCTGAAGCACCCACTCCTGTGCC
  ACTCCTCCAAGCCCAAGCTGGACACTCTTCAGAAACCCTATGTTCACAAAGAGAAACGGGCCCAGAGAAT
  CCCGATTCCACCCCCAAAGAAGACTAGAGCCCAACTTCTGGATGACATCTTCATTCGCTTGCGGGATCCC
  CGGAACATTACAGAGGCTCCACTAGGTGCTGTCCTGCACCAGTGGACAGAACGGCGGCTGGTGAACCACA
  AGCAGTACCTGGAGGCCAAGAGGCTGTTGAAGGAGTTCCAGGCACGTTACCGGCAGCTGGTGAGTGGCTC
  TCTGCGCACAGTGTTCGGGACTACCCCGCTCCCCATGGCCTGCCCAGCGCTGAGTGAGAGCCAGCCCAAG
  TTCGGCCACTTCCTCGAGTTCATGGATGAGTTCTGCCAGGAGCCCACAGCCAGTGACTCACAAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216596 representing NM_182969
  Red=Cloning site Green=Tags(s)
  
  MAFSGIYKLDDGKPYLNNCFPARNLLRVPEEGQGHWLVVQKGNLKKKPKGLVGAQAERRESLKATSFEFK
  GKKESRRENQVDLPGHILDQAFLLKHHCVRKPSDLCTINAKENDFKHFHSVIYINASENLLPLEAFHTFP
  ALKELDLAFNGIKTIYVKYGDFKLLEFLDLSFNSLTVEAICDLGILPHLRVLLLTGNGLTSLPPNLAVAE
  QEASVTSLTSKRYILRFPALETLMLDDNRLSNPSCFASLAGLRRLKKLSLDENRIIRIPYLQQVQLYDES
  VDWNGGRGSPHKEPQFMLQSKPRMLEDSDEQLDYTVLPMKKDVDRTEVVFSSYPGFSTSETTKICSLPPI
  FEILPVKSLKARNQTLAPPFPELRYLSLAYNKIAKEDAVLPVALFPSLCEFVFHNNPLVAHTRGVPPLLK
  SFLQERLGIHLIRRKIVKPKHHVLMSRKESWKVKSEIPKVPKQPLVLHHPRMTTTKSPSKDMLEPEAELA
  EDLPTTKSTSVESEMPTENLEGHSPSCRTFVPLPPICSNSTVHSEETLSHLSDTTVRLSPERPSDEDSKS
  TESIFLTQVSELPSSVIHKDDLELKEKDQKKPPTAPREVKGTRRKLPTAFLPSKYHGYEELLTAKPDPAF
  IEPKGIQKNAQALQQMLKHPLLCHSSKPKLDTLQKPYVHKEKRAQRIPIPPPKKTRAQLLDDIFIRLRDP
  RNITEAPLGAVLHQWTERRLVNHKQYLEAKRLLKEFQARYRQLVSGSLRTVFGTTPLPMACPALSESQPK
  FGHFLEFMDEFCQEPTASDSQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182969
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182969.4
• RefSeq Size: 5028 bp
• RefSeq ORF: 2379 bp
• Locus ID: 143570
• UniProt ID: Q6P2D8
• Cytogenetics: 11q13.4
• MW: 89.7 kDa
• Gene Summary: May be involved in the response of cells to X-ray radiation.[UniProtKB/Swiss-Prot Function]