MAEA (NM_005882) Human Tagged ORF Clone

CAT#: RC216473

MAEA (Myc-DDK-tagged)-Human macrophage erythroblast attacher (MAEA), transcript variant 2

Product Images

Circular map for RC216473

• 

  Western blot validation of overexpression lysate (Cat# LY417000) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216473 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAEA
• Synonyms: EMLP; EMP; GID9; HLC-10; P44EMLP; PIG5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216473 representing NM_005882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCAGGAGTCGGCGGCTCAGTTGTCCATGACCCTGAAGGTCCAGGAGTACCCGACCCTCAAGG
  TGCCCTACGAGACGCTGAACAAACGCTTTCGCGCCGCTCAGAAGAACATTGACCGGGAGACCAGCCACGT
  CACCATGGTGGTGGCCGAGCTGGAGAAGACGTTGAGCGGCTGCCCCGCCGTGGACTCCGTGGTCAGCCTG
  CTGGACGGCGTGGTGGAGAAGCTCAGCGTCCTCAAGAGGAAGGCGGTGGAATCCATCCAGGCCGAGGACG
  AGAGCGCCAAGCTGTGCAAGCGCCGGATCGAGCACCTCAAAGAGCATAGCAGCGACCAGCCCGCGGCGGC
  CAGCGTGTGGAAGAGGAAGCGCATGGATCGCATGATGGTGGAGCACCTGCTGCGTTGCGGCTACTACAAC
  ACGGCTGTCAAGCTGGCGCGCCAGAGCGGCATCGAGAGCTGCCTGGAGTTCAGCCTCAGAATCCAGGAGT
  TCATTGAACTCATCCGGCAGAATAAGAGACTGGACGCTGTGAGACATGCAAGAAAGCACTTCAGCCAAGC
  AGAAGGGAGCCAGCTGGACGAGGTGCGCCAGGCCATGGGCATGCTGGCCTTCCCGCCCGACACGCACATC
  TCCCCGTACAAGGACCTTCTGGACCCTGCACGGTGGCGGATGCTGATCCAGCAGTTCCGGTACGACAACT
  ACCGACTACACCAGCTGGGAAACAATTCTGTGTTCACCCTCACCCTGCAGGCTGGCCTCTCAGCCATCAA
  GACACCACAGTGCTACAAGGAGGACGGCAGCTCCAAGAGCCCTGACTGCCCTGTGTGCAGCCGCTCCCTG
  AACAAGCTGGCGCAGCCCCTGCCCATGGCCCACTGTGCCAACTCCCGCCTGGTCTGCAAGATTTCTGGCG
  ACGTGATGAACGAGAACAATCCGCCCATGATGCTGCCCAACGGCTACGTCTACGGCTACAATTCTCTGCT
  TTCTATCCGTCAAGATGATAAAGTCGTGTGCCCGAGAACCAAAGAAGTCTTCCACTTCTCACAAGCCGAG
  AAGGTGTACATCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216473 representing NM_005882
  Red=Cloning site Green=Tags(s)
  
  MAVQESAAQLSMTLKVQEYPTLKVPYETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSGCPAVDSVVSL
  LDGVVEKLSVLKRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAASVWKRKRMDRMMVEHLLRCGYYN
  TAVKLARQSGIESCLEFSLRIQEFIELIRQNKRLDAVRHARKHFSQAEGSQLDEVRQAMGMLAFPPDTHI
  SPYKDLLDPARWRMLIQQFRYDNYRLHQLGNNSVFTLTLQAGLSAIKTPQCYKEDGSSKSPDCPVCSRSL
  NKLAQPLPMAHCANSRLVCKISGDVMNENNPPMMLPNGYVYGYNSLLSIRQDDKVVCPRTKEVFHFSQAE
  KVYIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005882
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005882.5
• RefSeq Size: 1983 bp
• RefSeq ORF: 1068 bp
• Locus ID: 10296
• UniProt ID: Q7L5Y9
• Cytogenetics: 4p16.3
• Domains: LisH, CTLH
• Protein Families: Druggable Genome
• MW: 40.2 kDa
• Gene Summary: This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]