GIPC (GIPC1) (NM_005716) Human Tagged ORF Clone

CAT#: RC216466

GIPC1 (Myc-DDK-tagged)-Human GIPC PDZ domain containing family, member 1 (GIPC1), transcript variant 1

Product Images

Circular map for RC216466

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  Western blot validation of overexpression lysate (Cat# LY417114) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216466 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GIPC1 protein (Cat# TP316466). The protein was produced from HEK293T cells transfected with GIPC1 cDNA clone (Cat# RC216466) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GIPC
• Synonyms: C19orf3; GIPC; GLUT1CBP; Hs.6454; IIP-1; NIP; OPDM2; RGS19IP1; SEMCAP; SYNECTIIN; SYNECTIN; TIP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216466 representing NM_005716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGGGACTGGGGCGGCGGAAAAAGGCGCCCCCTCTAGTGGAAAATGAGGAGGCTGAGCCAGGCC
  GTGGAGGGCTGGGCGTGGGGGAGCCAGGGCCTCTGGGCGGAGGTGGGTCGGGGGGCCCCCAAATGGGCTT
  GCCCCCCCCTCCCCCAGCCCTGCGGCCCCGCCTCGTGTTCCACACCCAGCTGGCCCATGGCAGTCCCACT
  GGCCGCATCGAGGGCTTCACCAACGTCAAGGAGCTGTATGGCAAGATCGCCGAGGCCTTCCGCCTGCCAA
  CTGCCGAGGTGATGTTCTGCACCCTGAACACCCACAAAGTGGACATGGACAAGCTCCTGGGGGGCCAGAT
  CGGGCTGGAGGACTTCATCTTCGCCCACGTGAAGGGGCAGCGCAAGGAGGTGGAGGTGTTCAAGTCGGAG
  GATGCACTCGGGCTCACCATCACGGACAACGGGGCTGGCTACGCCTTCATCAAGCGCATCAAGGAGGGCA
  GCGTGATCGACCACATCCACCTCATCAGCGTGGGCGACATGATCGAGGCCATTAACGGGCAGAGCCTGCT
  GGGCTGCCGGCACTACGAGGTGGCCCGGCTGCTCAAGGAGCTGCCCCGAGGCCGTACCTTCACGCTGAAG
  CTCACGGAGCCTCGCAAGGCCTTCGACATGATCAGCCAGCGTTCAGCGGGTGGCCGCCCTGGCTCTGGCC
  CACAACTGGGCACTGGCCGAGGGACCCTGCGGCTCCGATCCCGGGGCCCCGCCACGGTGGAGGATCTGCC
  CTCTGCCTTTGAAGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACATGGGTATCAGGGAC
  ACGGAGCTGGCGGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGAACCCGGATGAGCTGGCCGAGGCCC
  TGGACGAACGGCTGGGTGACTTTGCCTTCCCTGACGAGTTCGTCTTTGACGTCTGGGGCGCCATTGGGGA
  CGCCAAGGTCGGCCGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216466 representing NM_005716
  Red=Cloning site Green=Tags(s)
  
  MPLGLGRRKKAPPLVENEEAEPGRGGLGVGEPGPLGGGGSGGPQMGLPPPPPALRPRLVFHTQLAHGSPT
  GRIEGFTNVKELYGKIAEAFRLPTAEVMFCTLNTHKVDMDKLLGGQIGLEDFIFAHVKGQRKEVEVFKSE
  DALGLTITDNGAGYAFIKRIKEGSVIDHIHLISVGDMIEAINGQSLLGCRHYEVARLLKELPRGRTFTLK
  LTEPRKAFDMISQRSAGGRPGSGPQLGTGRGTLRLRSRGPATVEDLPSAFEEKAIEKVDDLLESYMGIRD
  TELAATMVELGKDKRNPDELAEALDERLGDFAFPDEFVFDVWGAIGDAKVGRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005716
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005716.4
• RefSeq Size: 1947 bp
• RefSeq ORF: 1002 bp
• Locus ID: 10755
• UniProt ID: O14908
• Cytogenetics: 19p13.12
• Domains: PDZ
• Protein Families: Druggable Genome
• MW: 35.9 kDa
• Gene Summary: GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al., 2008 [PubMed 18775991]).[supplied by OMIM, Apr 2009]