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KIDINS220 (NM_020738) Human Tagged ORF Clone
CAT#: RC216441
KIDINS220 (Myc-DDK-tagged)-Human kinase D-interacting substrate, 220kDa (KIDINS220)
ORF Plasmid: tGFP
"NM_020738" in other vectors (2)
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Frequently bought together (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | KIDINS220 |
| Synonyms | ARMS; SINO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216441 representing NM_020738
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGTTTTGATATCACAGAGCGTCATAAATTATGTAGAGGAAGAAAACATTCCTGCTCTGAAAGCTC TTCTTGAAAAATGCAAAGATGTAGATGAGAGAAATGAGTGTGGCCAGACTCCACTGATGATAGCTGCCGA ACAAGGCAATCTGGAAATAGTGAAGGAATTAATTAAGAATGGAGCTAACTGCAATCTGGAAGATTTGGAT AATTGGACAGCACTTATATCTGCATCGAAAGAAGGGCATGTGCACATCGTAGAGGAACTACTGAAATGTG GGGTTAACTTGGAGCACCGTGATATGGGAGGATGGACAGCTCTTATGTGGGCATGTTACAAAGGCCGTAC TGACGTAGTAGAGTTGCTTCTTTCTCATGGTGCCAATCCAAGTGTCACTGGTCTGTACAGTGTTTACCCA ATCATTTGGGCAGCAGGGAGAGGCCATGCAGATATAGTTCATCTTTTACTGCAAAATGGTGCTAAAGTCA ACTGCTCTGATAAGTATGGAACCACCCCTTTAGTTTGGGCTGCACGAAAGGGTCATTTGGAATGTGTGAA ACATTTATTGGCCATGGGAGCTGATGTGGATCAAGAAGGAGCTAATTCAATGACTGCACTTATTGTGGCA GTGAAAGGAGGTTACACACAGTCAGTAAAAGAAATTTTGAAGAGGAATCCAAATGTAAACTTAACAGATA AAGATGGAAATACAGCTTTGATGATTGCATCAAAGGAGGGACATACGGAGATTGTGCAGGATCTGCTCGA CGCTGGAACATATGTGAACATACCTGACAGGAGTGGGGATACTGTGTTGATTGGCGCTGTCAGAGGTGGT CATGTTGAAATTGTTCGAGCGCTTCTCCAAAAATATGCTGATATAGACATTAGAGGACAGGATAATAAAA CTGCTTTGTATTGGGCTGTTGAGAAAGGAAATGCAACAATGGTGAGAGATATCTTACAGTGCAATCCTGA CACTGAAATATGCACAAAGGATGGTGAAACGCCACTTATAAAGGCTACCAAGATGAGAAACATTGAAGTG GTGGAGCTGCTGCTAGATAAAGGTGCTAAAGTGTCTGCTGTAGATAAGAAAGGAGATACTCCCTTGCATA TTGCTATTCGTGGAAGGAGCCGGAAACTGGCAGAACTGCTTTTAAGAAATCCCAAAGATGGGCGATTACT TTATAGGCCCAACAAAGCAGGCGAGACTCCTTATAATATTGACTGTAGCCATCAGAAGAGTATTTTAACT CAAATATTTGGAGCCAGACACTTGTCTCCTACTGAAACAGACGGTGACATGCTTGGATATGATTTATATA GCAGTGCCCTGGCAGATATTCTCAGTGAGCCTACCATGCAGCCACCCATTTGTGTGGGGTTATATGCACA GTGGGGAAGTGGGAAATCTTTCTTACTCAAGAAACTAGAAGACGAAATGAAAACCTTCGCCGGACAACAG ATTGAGCCTCTCTTTCAGTTCTCATGGCTCATAGTGTTTCTTACCCTGCTACTTTGTGGAGGGCTTGGTT TATTGTTTGCCTTCACGGTCCACCCAAATCTTGGAATAGCAGTGTCACTGAGCTTCTTGGCTCTCTTATA TATATTCTTTATTGTCATTTACTTTGGTGGACGAAGAGAAGGAGAGAGTTGGAATTGGGCCTGGGTCCTC AGCACTAGATTGGCAAGACATATTGGATATTTGGAACTCCTCCTTAAATTGATGTTTGTGAATCCACCTG AGTTGCCAGAGCAGACTACTAAAGCTTTACCTGTGAGGTTTTTGTTTACAGATTACAATAGACTGTCCAG TGTAGGTGGAGAAACTTCTCTGGCTGAAATGATTGCAACCCTCTCGGATGCTTGTGAAAGAGAGTTTGGC TTTTTGGCAACCAGGCTTTTTCGAGTATTCAAGACTGAAGATACTCAGGGTAAAAAGAAATGGAAAAAAA CATGTTGTCTCCCATCTTTTGTCATCTTCCTTTTTATCATTGGCTGCATTATATCTGGAATTACTCTTCT GGCTATATTTAGAGTTGACCCAAAGCATCTGACTGTAAATGCTGTCCTCATATCAATCGCATCTGTAGTG GGATTGGCCTTTGTGTTGAACTGTCGTACATGGTGGCAAGTGCTGGACTCGCTCCTGAATTCCCAAAGAA AACGCCTCCATAATGCAGCCTCCAAACTGCACAAATTGAAAAGTGAAGGATTCATGAAAGTTCTTAAATG TGAAGTGGAATTGATGGCCAGGATGGCAAAAACCATTGACAGCTTCACTCAGAATCAGACAAGGCTGGTG GTCATCATCGATGGATTAGATGCCTGTGAGCAGGACAAAGTCCTTCAGATGCTGGACACTGTCCGAGTTC TGTTTTCAAAAGGCCCGTTCATTGCCATTTTTGCAAGTGATCCACATATTATCATAAAGGCAATTAACCA GAACCTCAATAGTGTGCTTCGGGATTCAAATATAAATGGCCATGACTACATGCGCAACATAGTCCACTTG CCTGTGTTCCTTAATAGTCGTGGACTAAGCAATGCAAGAAAATTTCTCGTAACTTCAGCAACAAATGGAG ACGTTCCATGCTCAGATACTACAGGGATACAGGAAGATGCTGACAGAAGAGTTTCACAGAACAGCCTTGG GGAGATGACAAAACTTGGTAGCAAGACAGCCCTCAATAGACGGGACACTTACCGAAGAAGGCAGATGCAG AGGACCATCACTCGCCAGATGTCCTTTGATCTTACAAAACTGCTGGTTACCGAGGACTGGTTCAGTGACA TCAGTCCCCAGACCATGAGAAGATTACTTAATATTGTTTCTGTGACAGGACGATTACTGAGAGCCAATCA GATTAGTTTCAACTGGGACAGGCTTGCTAGCTGGATCAACCTTACTGAGCAGTGGCCATACCGGACTTCA TGGCTCATATTATATTTGGAAGAGACTGAAGGTATTCCAGATCAAATGACATTAAAAACCATCTACGAAA GAATATCAAAGAATATTCCAACAACTAAGGATGTTGAGCCACTTCTTGAAATTGATGGAGATATAAGAAA TTTTGAAGTGTTTTTGTCTTCAAGGACCCCAGTTCTTGTGGCTCGAGATGTAAAAGTCTTTTTGCCATGC ACTGTAAACCTAGATCCCAAACTACGGGAAATTATTGCAGATGTTCGTGCTGCCAGAGAGCAGATCAGTA TTGGAGGACTGGCGTACCCCCCGCTCCCTCTACATGAGGGTCCTCCTAGGGCGCCATCAGGGTACAGCCA GCCCCCATCCGTGTGCTCTTCCACGTCCTTCAATGGGCCCTTCGCAGGTGGAGTGGTGTCACCACAGCCT CACAGCAGCTATTACAGCGGCATGACGGGCCCTCAGCATCCCTTCTACAACAGGCCATTCTTTGCCCCAT ACCTTTACACGCCAAGGTATTACCCTGGCGGCTCCCAACATCTCATCTCACGTCCATCAGTAAAAACGAG TTTGCCCAGAGATCAGAACAATGGCCTAGAAGTTATCAAGGAGGATGCTGCTGAGGGGCTTTCTTCACCC ACAGACTCCTCGAGGGGGTCAGGCCCAGCCCCAGGCCCAGTGGTATTACTGAATTCACTGAATGTGGATG CAGTATGTGAGAAGCTGAAACAAATAGAAGGGCTGGACCAGAGTATGCTGCCTCAGTATTGTACCACGAT CAAAAAGGCAAACATAAATGGCCGTGTGTTAGCTCAGTGTAACATTGATGAGCTGAAGAAAGAGATGAAT ATGAATTTTGGAGACTGGCACCTTTTCAGAAGCACAGTACTAGAAATGAGAAACGCAGAAAGCCACGTGG TCCCTGAAGACCCACGTTTCCTCAGTGAGAGCAGCAGTGGCCCAGCCCCGCACGGTGAGCCTGCTCGCCG CGCTTCCCACAACGAGCTGCCTCACACCGAGCTCTCCAGCCAGACGCCCTACACACTCAACTTCAGCTTC GAAGAGCTGAACACGCTTGGCCTGGATGAAGGTGCCCCTCGTCACAGTAATCTAAGTTGGCAGTCACAAA CTCGCAGAACCCCAAGTCTTTCGAGTCTCAATTCCCAGGATTCCAGTATTGAAATTTCAAAGCTTACTGA TAAGGTGCAGGCCGAGTATAGAGATGCCTATAGAGAATACATTGCTCAGATGTCCCAGTTAGAAGGGGGC CCCGGGTCTACAACCATTAGTGGCAGATCTTCTCCACATAGCACATATTACATGGGTCAGAGTTCATCAG GGGGCTCTATTCATTCAAACCTAGAGCAAGAAAAGGGGAAGGATAGTGAACCAAAGCCCGATGATGGGAG GAAGTCCTTTCTAATGAAGAGGGGAGATGTTATCGATTATTCATCATCAGGGGTTTCCACCAACGATGCT TCCCCCCTGGATCCTATCACTGAAGAAGATGAAAAATCAGATCAGTCAGGCAGTAAGCTTCTCCCAGGCA AGAAATCTTCCGAAAGGTCAAGCCTCTTCCAGACAGATTTGAAGCTTAAGGGAAGTGGGCTGCGCTATCA AAAACTCCCAAGTGACGAGGATGAATCTGGCACAGAAGAATCAGATAACACTCCACTGCTCAAAGATGAC AAAGACAGAAAAGCCGAAGGGAAAGTAGAGAGAGTGCCGAAGTCTCCAGAACACAGTGCTGAGCCGATCA GAACCTTCATTAAAGCCAAAGAGTATTTATCGGATGCGCTCCTTGACAAAAAGGATTCATCGGATTCAGG AGTGAGATCCAGTGAAAGTTCTCCCAATCACTCTCTGCACAATGAAGTGGCGGATGACTCCCAGCTTGAA AAGGCAAATCTCATAGAGCTGGAAGATGACAGTCACAGCGGAAAGCGGGGAATCCCACATAGCCTGAGTG GCCTGCAAGATCCAATTATAGCTCGGATGTCCATTTGTTCAGAAGACAAGAAAAGCCCTTCCGAATGCAG CTTGATAGCCAGCAGCCCTGAAGAAAACTGGCCTGCATGCCAGAAAGCCTACAACCTGAACCGAACTCCC AGCACCGTGACTCTGAACAACAATAGTGCTCCAGCCAACAGAGCCAATCAAAATTTCGATGAGATGGAGG GAATTAGGGAGACTTCTCAAGTCATTTTGAGGCCTAGTTCCAGTCCCAACCCAACCACTATTCAGAATGA GAATCTAAAAAGCATGACACATAAGCGAAGCCAACGTTCAAGTTACACAAGGCTCTCCAAAGATCCTCCG GAGCTCCATGCAGCAGCCTCTTCTGAGAGCACAGGCTTTGGAGAAGAAAGAGAAAGCATTCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216441 representing NM_020738
Red=Cloning site Green=Tags(s) MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLMIAAEQGNLEIVKELIKNGANCNLEDLD NWTALISASKEGHVHIVEELLKCGVNLEHRDMGGWTALMWACYKGRTDVVELLLSHGANPSVTGLYSVYP IIWAAGRGHADIVHLLLQNGAKVNCSDKYGTTPLVWAARKGHLECVKHLLAMGADVDQEGANSMTALIVA VKGGYTQSVKEILKRNPNVNLTDKDGNTALMIASKEGHTEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGG HVEIVRALLQKYADIDIRGQDNKTALYWAVEKGNATMVRDILQCNPDTEICTKDGETPLIKATKMRNIEV VELLLDKGAKVSAVDKKGDTPLHIAIRGRSRKLAELLLRNPKDGRLLYRPNKAGETPYNIDCSHQKSILT QIFGARHLSPTETDGDMLGYDLYSSALADILSEPTMQPPICVGLYAQWGSGKSFLLKKLEDEMKTFAGQQ IEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVL STRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFG FLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNAVLISIASVV GLAFVLNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQTRLV VIIDGLDACEQDKVLQMLDTVRVLFSKGPFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHL PVFLNSRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTYRRRQMQ RTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTS WLILYLEETEGIPDQMTLKTIYERISKNIPTTKDVEPLLEIDGDIRNFEVFLSSRTPVLVARDVKVFLPC TVNLDPKLREIIADVRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQP HSSYYSGMTGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLEVIKEDAAEGLSSP TDSSRGSGPAPGPVVLLNSLNVDAVCEKLKQIEGLDQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMN MNFGDWHLFRSTVLEMRNAESHVVPEDPRFLSESSSGPAPHGEPARRASHNELPHTELSSQTPYTLNFSF EELNTLGLDEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQAEYRDAYREYIAQMSQLEGG PGSTTISGRSSPHSTYYMGQSSSGGSIHSNLEQEKGKDSEPKPDDGRKSFLMKRGDVIDYSSSGVSTNDA SPLDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDD KDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLE KANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTP STVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPP ELHAAASSESTGFGEERESIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020738 |
| ORF Size | 5313 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020738.4 |
| RefSeq Size | 7264 bp |
| RefSeq ORF | 5316 bp |
| Locus ID | 57498 |
| UniProt ID | Q9ULH0 |
| Cytogenetics | 2p25.1 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 196.4 kDa |
| Gene Summary | This gene encodes a transmembrane protein that is preferentially expressed in the nervous system where it controls neuronal cell survival, differentiation into exons and dendrites, and synaptic plasticity. The encoded protein interacts with membrane receptors, cytosolic signaling components, and cytoskeletal proteins, serving as a scaffold that mediates crosstalk between the neurotrophin pathway and several other intracellular signaling pathways. Aberrant expression of this gene is associated with the onset of various neuropsychiatric disorders and neurodegenerative diseases, including Alzheimer's disease. Naturally occurring mutations in this gene are associated with a syndrome characterized by spastic paraplegia, intellectual disability, nystagmus and obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017] |
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