RIMBP2 (NM_015347) Human Tagged ORF Clone

CAT#: RC216393

RIMBP2 (Myc-DDK-tagged)-Human RIMS binding protein 2 (RIMBP2)

Product Images

Circular map for RC216393

• 

  Coomassie blue staining of purified RIMBP2 protein (Cat# TP316393). The protein was produced from HEK293T cells transfected with RIMBP2 cDNA clone (Cat# RC216393) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIMBP2
• Synonyms: PPP1R133; RBP2; RIM-BP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216393 representing NM_015347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGAGGCGGCTGAACGGCGGCAGCAGCTGCAGTTGGAGCATGACCAGGCCCTGGCTGTTCTCAGTG
  CCAAGCAGCAGGAAATTGACCTTCTGCAGAAGTCCAAGGTTCGAGAGCTGGAAGAGAAATGCCGGACTCA
  AAGTGAGCAGTTCAACCTGCTGTCCCGGGACCTGGAGAAGTTCCGGCAGCACGCTGGCAAGATTGACCTG
  CTGGGTGGCAGCGCGGTGGCCCCCCTGGACATCTCCACGGCCCCCAGCAAGCCTTTCCCACAGTTCATGA
  ATGGCCTAGCCACCTCCCTCGGCAAAGGTCAGGAGAGCGCTATTGGAGGCAGCTCTGCGATCGGTGAATA
  TATCCGGCCCCTTCCGCAGCCTGGTGACAGGCCGGAGCCTCTGTCCGCCAAGCCCACCTTCCTGTCGAGA
  TCCGGTAGCGCAAGATGCAGATCTGAGTCAGACATGGAGAATGAACGGAATTCCAATACCTCCAAGCAGA
  GATACTCGGGGAAGGTCCACCTCTGTGTTGCCCGCTATAGTTACAACCCCTTCGATGGACCGAACGAGAA
  CCCCGAAGCTGAGCTGCCCCTCACGGCGGGAAAATACCTCTACGTCTATGGAGACATGGATGAGGATGGG
  TTCTATGAAGGAGAGCTCCTCGATGGCCAGAGGGGTCTGGTGCCCTCCAACTTCGTGGACTTTGTGCAGG
  ACAACGAGTCGCGGTTGGCAAGCACGCTGGGGAACGAGCAGGATCAGAACTTCATCAACCATTCCGGCAT
  CGGCCTGGAGGGAGAGCACATCCTGGACCTCCACTCCCCAACCCACATAGATGCGGGCATCACCGACAAC
  AGTGCCGGGACCCTGGACGTGAACATCGACGACATCGGAGAAGACATCGTGCCTTACCCTAGAAAAATCA
  CCCTCATCAAACAACTCGCCAAAAGTGTTATTGTGGGCTGGGAGCCCCCGGCGGTGCCACCAGGATGGGG
  AACGGTGAGCAGCTACAACGTCCTGGTGGACAAGGAGACACGCATGAACCTCACGCTGGGGAGCAGAACT
  AAAGCCCTCATCGAGAAGCTCAACATGGCAGCCTGCACCTACCGCATCTCCGTGCAGTGCGTCACCAGCA
  GGGGCAGCTCGGATGAGCTGCAGTGCACGCTGCTGGTGGGCAAGGACGTGGTGGTGGCCCCCTCCCACCT
  GCGGGTGGACAACATCACGCAGATCTCCGCCCAGCTCTCCTGGCTACCCACCAACAGCAACTACAGCCAC
  GTCATCTTCCTCAACGAGGAGGAGTTCGACATCGTCAAGGCCGCCAGGTACAAGTACCAGTTCTTCAATC
  TCAGGCCCAACATGGCCTATAAGGTGAAGGTTCTGGCCAAACCCCACCAGATGCCGTGGCAGCTCCCGCT
  GGAGCAAAGGGAGAAGAAGGAGGCCTTTGTGGAGTTCTCCACGTTGCCTGCAGGACCCCCAGCACCCCCA
  CAAGATGTTACCGTCCAGGCTGGGGTGACCCCCGCCACCATCCGGGTCTCCTGGAGACCACCTGTGCTGA
  CGCCCACCGGGCTGTCCAATGGCGCAAACGTTACCGGCTACGGCGTGTATGCCAAAGGGCAGAGGGTGGC
  TGAAGTCATCTTCCCCACGGCAGACAGCACGGCCGTGGAGCTTGTGCGGCTGCGGAGCCTGGAGGCCAAG
  GGCGTGACCGTGCGGACCCTCTCCGCCCAGGGCGAGTCCGTGGACTCTGCAGTTGCTGCCGTTCCCCCCG
  AGCTCCTGGTGCCTCCTACCCCCCACCCGAGACCTGCACCCCAATCAAAGCCATTAGCAAGTTCTGGAGT
  CCCCGAAACCAAAGACGAGCACCTGGGTCCCCACGCCAGGATGGATGAGGCCTGGGAGCAGAGCCGTGCA
  CCTGGCCCTGTGCATGGGCACATGCTGGAGCCGCCCGTGGGCCCCGGAAGGCGGTCGCCCTCACCCAGCC
  GCATCCTGCCACAGCCACAGGGCACCCCGGTGTCCACCACCGTCGCCAAGGCCATGGCCCGGGAGGCCGC
  GCAGAGGGTGGCCGAGAGCAGCAGGTTAGAGAAAAGGAGCGTCTTCCTAGAGAGAAGCAGCGCGGGGCAG
  TACGCCGCCTCAGACGAGGAGGACGCCTATGACTCTCCAGACTTCAAGAGGAGGGGCGCCTCGGTGGACG
  ACTTCCTGAAAGGCTCTGAACTTGGCAAGCAGCCGCACTGTTGCCATGGAGACGAGTACCACACAGAGAG
  CAGCCGGGGGTCTGACCTCTCAGACATCATGGAGGAGGACGAGGAGGAGCTGTATTCTGAAATGCAGCTG
  GAAGATGGGGGAAGGAGGCGGCCCAGCGGCACGTCCCACAATGCCCTCAAGATTTTAGGGAACCCAGCCT
  CTGCAGGACGGGTGGATCACATGGGCCGGAGGTTTCCCCGTGGCAGCGCTGGTCCTCAGAGGTCCCGGCC
  CGTGACAGTCCCATCCATCGACGATTACGGGCGAGACCGCCTTTCTCCAGACTTCTATGAAGAGTCAGAA
  ACTGACCCTGGTGCCGAAGAGCTCCCGGCCCGGATCTTTGTGGCTCTCTTTGACTACGACCCGCTCACCA
  TGTCCCCAAACCCAGATGCTGCAGAGGAGGAGCTTCCCTTTAAAGAAGGCCAGATCATCAAGGTTTATGG
  TGATAAAGACGCTGATGGATTCTACCGTGGGGAAACCTGTGCCCGGCTTGGCCTTATTCCTTGTAACATG
  GTCTCTGAGATACAAGCAGATGATGAGGAGATGATGGATCAGCTTCTTAGACAGGGCTTTCTCCCTCTGA
  ATACACCTGTGGAGAAAATAGAGAGAAGCAGGAGAAGTGGCAGGCGTCATTCGGTATCGACGCGGAGAAT
  GGTGGCCCTGTATGACTACGACCCCAGAGAAAGCTCGCCCAACGTCGATGTCGAGGCCGAACTTACATTT
  TGCACAGGAGATATTATTACAGTTTTTGGTGAAATTGATGAAGATGGATTTTATTATGGGGAGCTGAATG
  GGCAGAAAGGCCTTGTGCCCTCAAACTTCTTGGAAGAAGTGCCTGATGACGTAGAAGTCTATCTTTCTGA
  TGCTCCATCCCACTACTCTCAAGATACGCCAATGCGCTCAAAGGCAAAAAGGAAGAAGAGTGTTCATTTC
  ACACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216393 representing NM_015347
  Red=Cloning site Green=Tags(s)
  
  MREAAERRQQLQLEHDQALAVLSAKQQEIDLLQKSKVRELEEKCRTQSEQFNLLSRDLEKFRQHAGKIDL
  LGGSAVAPLDISTAPSKPFPQFMNGLATSLGKGQESAIGGSSAIGEYIRPLPQPGDRPEPLSAKPTFLSR
  SGSARCRSESDMENERNSNTSKQRYSGKVHLCVARYSYNPFDGPNENPEAELPLTAGKYLYVYGDMDEDG
  FYEGELLDGQRGLVPSNFVDFVQDNESRLASTLGNEQDQNFINHSGIGLEGEHILDLHSPTHIDAGITDN
  SAGTLDVNIDDIGEDIVPYPRKITLIKQLAKSVIVGWEPPAVPPGWGTVSSYNVLVDKETRMNLTLGSRT
  KALIEKLNMAACTYRISVQCVTSRGSSDELQCTLLVGKDVVVAPSHLRVDNITQISAQLSWLPTNSNYSH
  VIFLNEEEFDIVKAARYKYQFFNLRPNMAYKVKVLAKPHQMPWQLPLEQREKKEAFVEFSTLPAGPPAPP
  QDVTVQAGVTPATIRVSWRPPVLTPTGLSNGANVTGYGVYAKGQRVAEVIFPTADSTAVELVRLRSLEAK
  GVTVRTLSAQGESVDSAVAAVPPELLVPPTPHPRPAPQSKPLASSGVPETKDEHLGPHARMDEAWEQSRA
  PGPVHGHMLEPPVGPGRRSPSPSRILPQPQGTPVSTTVAKAMAREAAQRVAESSRLEKRSVFLERSSAGQ
  YAASDEEDAYDSPDFKRRGASVDDFLKGSELGKQPHCCHGDEYHTESSRGSDLSDIMEEDEEELYSEMQL
  EDGGRRRPSGTSHNALKILGNPASAGRVDHMGRRFPRGSAGPQRSRPVTVPSIDDYGRDRLSPDFYEESE
  TDPGAEELPARIFVALFDYDPLTMSPNPDAAEEELPFKEGQIIKVYGDKDADGFYRGETCARLGLIPCNM
  VSEIQADDEEMMDQLLRQGFLPLNTPVEKIERSRRSGRRHSVSTRRMVALYDYDPRESSPNVDVEAELTF
  CTGDIITVFGEIDEDGFYYGELNGQKGLVPSNFLEEVPDDVEVYLSDAPSHYSQDTPMRSKAKRKKSVHF
  TP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015347
• ORF Size: 3156 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015347.5
• RefSeq Size: 6322 bp
• RefSeq ORF: 3159 bp
• Locus ID: 23504
• UniProt ID: O15034
• Cytogenetics: 12q24.33
• MW: 115.8 kDa
• Gene Summary: Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B.[UniProtKB/Swiss-Prot Function]