FBXO10 (NM_012166) Human Tagged ORF Clone

CAT#: RC216136

FBXO10 (Myc-DDK-tagged)-Human F-box protein 10 (FBXO10)

Product Images

Circular map for RC216136

• 

  Western blot validation of overexpression lysate (Cat# LY415944) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216136 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXO10
• Synonyms: FBX10; PRMT11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216136 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGGTGGCCTCCCCTTGGAGCTGTGGCGCATGATCTTAGCCTACTTGCACCTTCCCGACCTGG
  GCCGCTGCAGCCTGGTATGCAGGGCCTGGTATGAACTGATCCTCAGTCTCGACAGCACCCGCTGGCGGCA
  GCTGTGTCTGGGTTGCACCGAGTGCCGCCATCCCAATTGGCCCAACCAGCCAGATGTGGAGCCTGAGTCT
  TGGAGAGAAGCCTTCAAGCAGCATTACCTTGCATCCAAGACATGGACCAAGAATGCCTTGGACTTGGAGT
  CTTCCATCTGCTTTTCTCTATTCCGCCGGAGGAGGGAACGACGTACCCTGAGTGTTGGGCCAGGCCGTGA
  GTTTGACAGCCTGGGCAGTGCCTTGGCCATGGCCAGCCTGTATGACCGAATTGTGCTCTTCCCAGGTGTG
  TACGAAGAGCAAGGTGAAATCATCTTGAAGGTGCCTGTGGAGATTGTAGGGCAGGGGAAGTTGGGTGAAG
  TGGCCCTGCTGGCCAGCATTGATCAGCACTGCTCAACCACACGCCTGTGCAACCTCGTCTTCACGCCAGC
  CTGGTTCTCACCCATCATGTATAAGACAACATCAGGTCACGTCCAGTTTGACAACTGCAACTTTGAGAAC
  GGGCACATCCAGGTCCATGGCCCGGGTACTTGCCAAGTGAAGTTCTGTACCTTCAAAAACACCCATATCT
  TCCTGCACAACGTGCCCCTGTGTGTCCTGGAAAACTGTGAATTTGTGGGCAGTGAAAACAACTCTGTGAC
  TGTTGAGGGTCACCCATCTGCAGATAAGAACTGGGCCTACAAGTATCTACTAGGGCTTATCAAGTCCTCA
  CCCACTTTTCTCCCCACAGAGGACTCTGACTTTTTAATGTCCCTGGACCTAGAGAGCCGGGACCAGGCCT
  GGAGCCCAAAGACCTGTGACATTGTTATCGAGGGCAGCCAGAGCCCTACCAGCCCAGCTTCTAGCTCCCC
  AAAGCCAGGCTCCAAGGCTGGCTCACAGGAGGCAGAGGTGGGTAGTGATGGTGAAAGGGTGGCCCAGACC
  CCGGACAGCAGCGATGGAGGCCTGAGTCCCAGCGGTGAGGATGAAGATGAGGACCAGCTGATGTACAGAC
  TATCCTACCAAGTGCAGGGCCCACGCCCTGTATTGGGGGGCTCATTTCTGGGCCCACCTCTACCAGGAGC
  ATCCATTCAGCTGCCCAGCTGCCTAGTGCTGAACTCACTGCAGCAGGAGCTGCAGAAGGATAAGGAGGCC
  ATGGCACTGGCCAACTCCGTGCAGGGCTGCCTCATCCGCAAGTGCCTCTTCCGGGACGGGAAGGGAGGCG
  TCTTCGTCTGCTCCCACGGCAGAGCCAAGATGGAAGGAAACATCTTCCGGAACCTGACTTACGCAGTGCG
  GTGTATACATAATAGCAAGATCATCATGCTCAGGAACGACATTTACCGCTGCCGAGCGTCAGGCATCTTT
  CTTCGCTTGGAGGGCGGTGGCTTGATTGCCGGCAACAACATTTACCACAATGCAGAGGCTGGTGTAGACA
  TCCGGAAAAAGTCCAACCCACTCATACTGTGTAACCAGATCCACCATGGCCTTCGCTCTGGCATTGTCGT
  CCTTGGCAATGGGAAAGGCATCATCCGGAACAATCAAATCTTTTCCAATAAGGAGGCTGGCATTTACATC
  CTGTACCACGGAAACCCCGTTGTGAGCGGGAACCACATCTTCAAGGGCCGTGCTGCCGGCATAGCAGTGA
  ATGAGAACGGCAAAGGCCTCATCACAGAAAATGTCATCCGTGAGAATCAGTGGGGAGGTGTGGACATCCG
  CCGTGGAGGGATCCCCGTTCTCAGGAGTAACCTCATCTGCTTTGGCTATTCAGATGGTGTGGTTGTGGGA
  GACGAAGGCAAAGGCCTCATAGAAGGAAATACCATCTACGCTAACAAGGGCTGTGGTGTGTGGATGATGT
  CGTCCAGCCTCCCCCATGTCACCAGCAATCACGTCAGCTACAATGGCCTGTATGGAGTGGCAGTATTTAG
  CCAGAAGGATGGCTCCAGCGAGTTACCTCGAGGCCACAGGGCTCAAGAGAACTTCAGCGAGGATGGGGAC
  GCCATCCTCTGGGAGACAGAGCTGGAGAAGGAGGACGACCCACTGCGCCGGCCCATCACCATAGCTCTTG
  TTGAGTCTAACAGTATTAATCACAATGGAGCCTCAGGACTCTATGTCCAGAGCAGCGAGGCACTGCATGT
  CATCACCAATGTGATCCACGCGAATGGGGACAGAGGCATTACTGTGGCCCAGAGCAGCCAACCCACCCGA
  GTGGCCAACAACAGCATCTCCTGCAACCGGCAAAGTGGGGTCAAGGTTGAGGCCCAGTGCAAAGTGGAGC
  TCCGGGGCAATGGTATCTATGACAACAGAGGCCACGGCATTATCACCAAGGGCGACAGCACCATCGTCAT
  TGAAAACGATATCATTGGCAACCGGGGCAGCGGGCTGCAGCTGCTGCCCAGGTCCGACACTAAAGTAATA
  AAGAACCGGATCCACTCGTTCCGGGCCTACGGCATCGCCGTGCGGGGCCGTGCCAAGGCCCTGGTGCAGG
  AAAACATCATCTTCCAGGGCAAAACCAGTAAGACCATCTTTCAGCAGATCTCAAACAACCGAGAATGCAT
  CATGCAAAACAACAAGTTCCTGGTCTTCAAGAAAAAGTCTGATACGTGGCGCCTGGTGAACCCACCAGCA
  CGGCCCCACCTTGAAAATTCTCTCAGACGTCCCTCGGCAGCCCACAATGGGCAGAAGGTGACAGCCATGG
  CAACGAGGATCACAGCCCGGGTGGAAGGTGGTTACCACAGCAACCGCAGTGTCTTCTGCACCATCCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216136 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAGGLPLELWRMILAYLHLPDLGRCSLVCRAWYELILSLDSTRWRQLCLGCTECRHPNWPNQPDVEPES
  WREAFKQHYLASKTWTKNALDLESSICFSLFRRRRERRTLSVGPGREFDSLGSALAMASLYDRIVLFPGV
  YEEQGEIILKVPVEIVGQGKLGEVALLASIDQHCSTTRLCNLVFTPAWFSPIMYKTTSGHVQFDNCNFEN
  GHIQVHGPGTCQVKFCTFKNTHIFLHNVPLCVLENCEFVGSENNSVTVEGHPSADKNWAYKYLLGLIKSS
  PTFLPTEDSDFLMSLDLESRDQAWSPKTCDIVIEGSQSPTSPASSSPKPGSKAGSQEAEVGSDGERVAQT
  PDSSDGGLSPSGEDEDEDQLMYRLSYQVQGPRPVLGGSFLGPPLPGASIQLPSCLVLNSLQQELQKDKEA
  MALANSVQGCLIRKCLFRDGKGGVFVCSHGRAKMEGNIFRNLTYAVRCIHNSKIIMLRNDIYRCRASGIF
  LRLEGGGLIAGNNIYHNAEAGVDIRKKSNPLILCNQIHHGLRSGIVVLGNGKGIIRNNQIFSNKEAGIYI
  LYHGNPVVSGNHIFKGRAAGIAVNENGKGLITENVIRENQWGGVDIRRGGIPVLRSNLICFGYSDGVVVG
  DEGKGLIEGNTIYANKGCGVWMMSSSLPHVTSNHVSYNGLYGVAVFSQKDGSSELPRGHRAQENFSEDGD
  AILWETELEKEDDPLRRPITIALVESNSINHNGASGLYVQSSEALHVITNVIHANGDRGITVAQSSQPTR
  VANNSISCNRQSGVKVEAQCKVELRGNGIYDNRGHGIITKGDSTIVIENDIIGNRGSGLQLLPRSDTKVI
  KNRIHSFRAYGIAVRGRAKALVQENIIFQGKTSKTIFQQISNNRECIMQNNKFLVFKKKSDTWRLVNPPA
  RPHLENSLRRPSAAHNGQKVTAMATRITARVEGGYHSNRSVFCTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012166
• ORF Size: 2870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012166.2, NP_036298.2
• RefSeq Size: 4592 bp
• RefSeq ORF: 2871 bp
• Locus ID: 26267
• UniProt ID: Q9UK96
• Cytogenetics: 9p13.2
• Protein Families: Druggable Genome
• MW: 105.2 kDa
• Gene Summary: Members of the F-box protein family, such as FBXO10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]