AKNAD1 (NM_152763) Human Tagged ORF Clone

CAT#: RC215937

AKNAD1 (Myc-DDK-tagged)-Human AKNA domain containing 1 (AKNAD1)

Product Images

Circular map for RC215937

• 

  Western blot validation of overexpression lysate (Cat# LY407313) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215937 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKNAD1
• Synonyms: C1orf62
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215937 representing NM_152763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGCTGATTTTTCAGAACACACGACTTATAAGCAGGAGGATTTGCCTTATGATGGGGACCTCT
  CTCAGATTAAGATAGGCAATGATTACAGTTTTACCTCAAAAAAGGATGGCCTTGAAGTCTTAAATCAAAT
  TATTTTCATAGCAGATGACCCTCAAGAGAAGGCTATGCATAGTGAGACTTGTGGAAATACAGCTGTGACC
  ATACCCCTGGGTAAAATTACTGAAAATGCTGCCAACAAAAAAGACGAGAAAGAGAAACAATGCACTGCAG
  CTCTTCATATTCCAGCAAATGAAGGAGACGCTTCTAAGTCAAGCATTTCTGATATTTTACTTCATCATCT
  TTCCAAAGAGCCATTCTTAAGAGGTCAAGGCATTGATTGTGAAACCCTCCCAGAGATCTCAAATGCCGAC
  AGTTTTGAAGAGGAAGCTATTATTAAAAGTATTATTTCATGTTATAATAAGAATTCTTGGCCAAAAGAAC
  AAACCCCAGAACTCACTGACCAACTCAACCCGAAAAGGGATGGTGAAAACAGCAATAAGCCTGGTTCTGC
  CACCACGACAGAGGAAAATACCTCTGATTTAGAAGGGCCAGTGGCTGCTGGAGATAGCAGCCATCAAGAA
  AATGTGAATGTTCTAACTAAAACCAAGGGTCCAGGTGATAAACAAAAAAGTTATCAAGGGCAGTCACCCC
  AGAAACAGCAGACTGAAAAAGCAAATTCAGGCAACACGTTCAAATACGGCCAAGGTCAAGTTCATTACCA
  GCTCCCTGATTTCTCTAAGATTGCTCCCAAAGTGAAAATTCCTAAAAATAAGATAATTAATAAACCACTT
  GCAATAGCTAAACAAGCCAGCTTTTCTTCCAAGTCGAGAGATAAACCCACTCTTGTGCAAGATAGTCTAG
  AAACCACGCCTGAGTCAAACTGTGTTGAAAAACAACATCAAGAGCAGAAAGGGAAAATCACTGAACCTTC
  ACAACAAATCCAGATGGAGCCCATAGTACATATCCACCAAGAACTTCTCACAGGAATAGAATCTGAGGCA
  AGTCTCTCTAAGTTGTCACCAACCTCTCAGAAAGGCACTTCCTCAAGTTCTTCTTACATATTTCAAAAGA
  TATCCCAAGGGAAACAGATGTGTCAGAAGTTGAAAGAACAGACTGATCAACTGAAGACTAAAGTACAAGA
  ATTTTCCAAAAGAATAAAACAGGACTCTCCTTACCATTTGCAAGACAAGAAGCTGGTCCTGGAGAAACTG
  CAGGGACACCTTGAACTGCTGGAGCAGAACTTTCTGGCCACCAAGGACAAGCATCTGACTTTGCAGCAGC
  AAGTCCACAAGCACGAATCAACAATCGTTGGTGACTTTGATCCAGAAAGAAAAGTGGAAGGTGAAATCTT
  CAAATTGGAGATGCTGCTTGAAGATGTTAAAGAGAAAATGGATGAAAGCAAATATACTTCAGCTCCTTCC
  CTTCCTGTGAGTTCTCCAGTTACCCTGGATGACTTGGCCTCCACCTTCTCTTCACTCTCAAATGAGATTC
  CCAAGGAGCACCCCGGCCACCCTTCTGGGCCTCGAGGCTCAGGTGGGAGTGAGGTAACAGGGACACCCCA
  AGGAGGGCCGCAGGAGGCCCCCAACGAGGAGCTCTGTGAGCTGGCCCCGCAGACTTACCTAAACGGTCAT
  TATGGAGATGCAGCTGCCCAGAACAAGCCAGACCAAGTGGCCATGAGGCTGTCTTCTAACTCTGGGGAGG
  ATCCCAACGGCACTCCAAGAAGGCAGGATTGTGCAGAGATGACGGCACCCAGTCCGAGCTGTGCCTTCTG
  TCGCAGGCTCCTTGAATGGAAGCAAAACGTGGAGAAAAAGGGCCACGGAAGGATCAACTGTGGAAGATTT
  TCAATTGTCCTTCATGAAAAGGCACCACACTCAGATTCAACTCCCAATTCTGATACAGGACACAGCTTCT
  GTTCTGATTCTGGCACTGAAATGCAGAGTAACAAATGTCAGGACTGTGGCACTAAGATTCCTACCTCCCG
  AAGAGCCTGCAGGAAAGAACCAACTAAAGAATTTCATTATAGATACAACACTCCAGGACAGAATTACTCA
  AATCATAGCAAAAGAGGTGCCTTTGTCCAGCCCCATTCTTTAGATGAAAGTAAAAACTCTTCACCCTCTT
  TTTTAAAACCCAAACGGATCTGTTCTCAGAGAGTGAATTCAAAATCCTTTAAAGGTGAACATGAGCCCAC
  ACCAGGAAAAAAAAAACTTCAGGCTTTCATGACCTACAGCTCAGACCCTGCAACACCCTCACCCCATTTC
  TACTCCTGCAGGATTTCTGGAAGCAAATCCTTATGTGACTTTGATAGCACTGAAGAAATAAAATCTGAGA
  TTTTAAACTCGGCTTTGGATCATGCCCTAAGGACAGCAACCATTTTGAAAGAAACTACAGATCAAATGAT
  TAAAACGATTGCAGAAGACCTTGCTAAAGCACAGAGGTGGAGGAATCGACTGAAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215937 representing NM_152763
  Red=Cloning site Green=Tags(s)
  
  MDEADFSEHTTYKQEDLPYDGDLSQIKIGNDYSFTSKKDGLEVLNQIIFIADDPQEKAMHSETCGNTAVT
  IPLGKITENAANKKDEKEKQCTAALHIPANEGDASKSSISDILLHHLSKEPFLRGQGIDCETLPEISNAD
  SFEEEAIIKSIISCYNKNSWPKEQTPELTDQLNPKRDGENSNKPGSATTTEENTSDLEGPVAAGDSSHQE
  NVNVLTKTKGPGDKQKSYQGQSPQKQQTEKANSGNTFKYGQGQVHYQLPDFSKIAPKVKIPKNKIINKPL
  AIAKQASFSSKSRDKPTLVQDSLETTPESNCVEKQHQEQKGKITEPSQQIQMEPIVHIHQELLTGIESEA
  SLSKLSPTSQKGTSSSSSYIFQKISQGKQMCQKLKEQTDQLKTKVQEFSKRIKQDSPYHLQDKKLVLEKL
  QGHLELLEQNFLATKDKHLTLQQQVHKHESTIVGDFDPERKVEGEIFKLEMLLEDVKEKMDESKYTSAPS
  LPVSSPVTLDDLASTFSSLSNEIPKEHPGHPSGPRGSGGSEVTGTPQGGPQEAPNEELCELAPQTYLNGH
  YGDAAAQNKPDQVAMRLSSNSGEDPNGTPRRQDCAEMTAPSPSCAFCRRLLEWKQNVEKKGHGRINCGRF
  SIVLHEKAPHSDSTPNSDTGHSFCSDSGTEMQSNKCQDCGTKIPTSRRACRKEPTKEFHYRYNTPGQNYS
  NHSKRGAFVQPHSLDESKNSSPSFLKPKRICSQRVNSKSFKGEHEPTPGKKKLQAFMTYSSDPATPSPHF
  YSCRISGSKSLCDFDSTEEIKSEILNSALDHALRTATILKETTDQMIKTIAEDLAKAQRWRNRLKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152763
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152763.5
• RefSeq Size: 3063 bp
• RefSeq ORF: 2511 bp
• Locus ID: 254268
• UniProt ID: Q5T1N1
• Cytogenetics: 1p13.3
• MW: 92.9 kDa
• Gene Summary: This gene encodes a protein which contains a domain found in an AT-hook-containing transcription factor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]