TSHZ1 (NM_005786) Human Tagged ORF Clone

CAT#: RC215916

TSHZ1 (Myc-DDK-tagged)-Human teashirt zinc finger homeobox 1 (TSHZ1)

Product Images

Circular map for RC215916

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TSHZ1 (Cat# RC215916, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSHZ1 (Cat# TA809993)(1:2000). Positive lysates LY417073 (100ug) and LC417073 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY417073) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215916 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TSHZ1
• Synonyms: CAA; NY-CO-33; SDCCAG33; TSH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215916 representing NM_005786
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAATGAAGAGACGGAGATCAAAGAGGCGCAGAGCTACCAGAACTCCCCAGTCAGCTCTGCGACTA
  ACCAGGACGCCGGCTACGGGTCGCCCTTCAGTGAGAGCAGCGACCAGCTAGCCCATTTCAAAGGCTCTTC
  CTCTCGAGAAGAGAAGGAGGATCCGCAGTGTCCCGACAGCGTCTCGTACCCCCAGGACAGCCTGGCACAG
  ATCAAAGCTGTGTATGCAAACTTGTTCTCCGAGTCCTGCTGGTCCAGCTTAGCTCTGGATTTAAAGAAGT
  CGGGTTCCACCACCAGCACCAACGATGCCAGCCAGAAGGAGAGCTCCGCCCCCACCCCCACACCCCCCAC
  CTGCCCCGTCAGCACCACTGGCCCCACCACGAGCACGCCCAGCACCAGCTGCAGCTCCAGCACCAGCCAC
  AGCAGTACCACCAGTACCAGCAGCAGCTCCGGGTACGACTGGCACCAGGCTGCACTGGCCAAGACGCTGC
  AGCAGACGTCCTCGTATGGGCTGCTTCCTGAGCCCAGCCTGTTCAGCACCGTGCAGCTCTACCGCCAGAA
  CAACAAGCTCTACGGCTCCGTCTTCACGGGCGCCAGCAAGTTCCGGTGCAAAGACTGCAGTGCCGCGTAC
  GACACGCTGGTGGAACTGACGGTGCACATGAACGAGACAGGCCACTACCGTGACGACAACAGGGACAAGG
  ACTCCGAGAAGACCAAGAGGTGGTCCAAGCCCAGGAAGCGCTCCCTGATGGAGATGGAGGGGAAGGAGGA
  TGCCCAGAAGGTGCTGAAGTGCATGTACTGTGGACACTCCTTTGAGTCCTTGCAGGACCTCAGCGTCCAC
  ATGATCAAAACCAAGCATTACCAGAAAGTGCCTCTGAAGGAGCCAGTGCCAGCCATCACCAAACTGGTCC
  CCTCCACCAAAAAGCGGGCGCTTCAGGACCTGGCGCCCCCCTGCTCCCCTGAGCCAGCAGGAATGGCCGC
  AGAGGTGGCCCTGAGTGAGTCAGCCAAGGATCAGAAAGCAGCGAACCCGTACGTCACGCCCAATAACCGC
  TATGGCTACCAGAATGGCGCCAGCTACACCTGGCAGTTTGAGGCCCGCAAGGCGCAGATCCTCAAGTGCA
  TGGAGTGTGGCAGCTCCCACGACACGCTGCAGCAGCTCACCGCCCACATGATGGTCACCGGGCACTTCCT
  GAAAGTGACCACCTCGGCTTCTAAGAAGGGCAAGCAGTTGGTGCTGGACCCTGTGGTGGAAGAGAAGATC
  CAGTCCATCCCACTACCGCCCACCACCCACACGCGGCTGCCGGCCTCCAGCATCAAAAAGCAGCCCGACT
  CTCCCGCGGGGTCCACGACTTCTGAAGAAAAGAAAGAGCCAGAGAAGGAGAAGCCGCCTGTGGCTGGCGA
  CGCGGAGAAGATCAAGGAGGAGAGTGAGGACAGCTTGGAGAAATTTGAGCCCAGCACCCTGTACCCGTAC
  CTGCGTGAGGAGGACCTGGACGACAGCCCCAAGGGAGGGCTGGACATTCTCAAGTCCCTGGAGAATACCG
  TCTCCACGGCCATTAGCAAAGCTCAGAATGGTGCGCCCTCATGGGGTGGCTACCCCAGCATCCATGCAGC
  CTACCAGCTCCCGGGCACCGTGAAGCCACTGCCGGCGGCCGTGCAGAGCGTGCAGGTGCAGCCGTCCTAT
  GCTGGCGGCGTGAAGTCGCTGTCTTCCGCCGAGCACAACGCCCTCCTGCACTCCCCAGGGAGCCTCACGC
  CCCCACCGCACAAGAGCAACGTGTCTGCCATGGAGGAGCTGGTGGAGAAGGTCACGGGCAAGGTCAACAT
  CAAGAAGGAGGAGAGACCCCCTGAGAAGGAGAAGAGCTCCCTGGCCAAGGCTGCGTCCCCCATAGCAAAA
  GAGAATAAAGATTTCCCGAAAACGGAGGAAGTCAGCGGCAAACCACAGAAGAAGGGCCCTGAGGCCGAGA
  CTGGGAAGGCCAAAAAGGAGGGACCGCTGGACGTTCACACCCCAAATGGCACAGAGCCTCTCAAAGCAAA
  GGTCACCAACGGCTGTAACAACCTGGGGATCATCATGGACCACTCACCGGAGCCTTCCTTCATCAACCCG
  CTGAGCGCTTTGCAGTCCATCATGAACACCCACCTGGGCAAGGTGTCCAAGCCCGTGAGTCCCTCGCTGG
  ACCCGCTGGCGATGCTGTACAAGATCAGCAACAGCATGCTGGACAAGCCGGTGTACCCCGCCACCCCTGT
  GAAGCAGGCCGATGCCATCGACCGCTACTATTATGAAAACAGCGACCAGCCCATTGACTTAACCAAGTCC
  AAGAACAAGCCGCTGGTGTCCAGCGTGGCTGATTCGGTGGCATCACCTCTGCGGGAGAGCGCACTCATGG
  ACATCTCCGACATGGTGAAAAACCTCACAGGCCGCCTGACGCCCAAGTCCTCCACGCCCTCCACAGTTTC
  AGAGAAGTCCGATGCTGATGGCAGCAGCTTTGAGGAGGCGTTGGACGAGCTGTCACCGGTCCACAAGAGG
  AAGGGCCGGCAGTCCAACTGGAACCCGCAGCACCTTCTCATCCTGCAGGCCCAGTTCGCCTCGAGCTTGC
  GGGAGACCACAGAGGGCAAGTACATCATGTCGGACTTGGGCCCGCAGGAGAGGGTGCACATCTCGAAGTT
  TACTGGGCTCTCCATGACCACCATCAGCCACTGGCTGGCCAATGTGAAGTACCAGTTGAGGAGGACAGGG
  GGAACGAAATTCCTAAAGAACCTGGACACAGGGCATCCTGTTTTCTTTTGCAACGATTGTGCCTCTCAGT
  TCAGAACTGCTTCTACATACATAAGTCATTTGGAGACACACTTGGGCTTCAGCCTGAAGGATCTCTCCAA
  GCTGCCACTCAATCAGATTCAAGAACAGCAGAATGTTTCGAAAGTCCTCACCAACAAAACTCTGGGCCCA
  CTGGGGGCCACCGAGGAAGACTTGGGCTCCACATTCCAATGTAAGCTCTGCAACCGGACTTTTGCGAGCA
  AGCACGCAGTCAAACTGCACCTTAGTAAGACCCACGGCAAGTCTCCCGAGGACCACCTGATCTATGTGAC
  TGAGTTGGAGAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215916 representing NM_005786
  Red=Cloning site Green=Tags(s)
  
  MCNEETEIKEAQSYQNSPVSSATNQDAGYGSPFSESSDQLAHFKGSSSREEKEDPQCPDSVSYPQDSLAQ
  IKAVYANLFSESCWSSLALDLKKSGSTTSTNDASQKESSAPTPTPPTCPVSTTGPTTSTPSTSCSSSTSH
  SSTTSTSSSSGYDWHQAALAKTLQQTSSYGLLPEPSLFSTVQLYRQNNKLYGSVFTGASKFRCKDCSAAY
  DTLVELTVHMNETGHYRDDNRDKDSEKTKRWSKPRKRSLMEMEGKEDAQKVLKCMYCGHSFESLQDLSVH
  MIKTKHYQKVPLKEPVPAITKLVPSTKKRALQDLAPPCSPEPAGMAAEVALSESAKDQKAANPYVTPNNR
  YGYQNGASYTWQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFLKVTTSASKKGKQLVLDPVVEEKI
  QSIPLPPTTHTRLPASSIKKQPDSPAGSTTSEEKKEPEKEKPPVAGDAEKIKEESEDSLEKFEPSTLYPY
  LREEDLDDSPKGGLDILKSLENTVSTAISKAQNGAPSWGGYPSIHAAYQLPGTVKPLPAAVQSVQVQPSY
  AGGVKSLSSAEHNALLHSPGSLTPPPHKSNVSAMEELVEKVTGKVNIKKEERPPEKEKSSLAKAASPIAK
  ENKDFPKTEEVSGKPQKKGPEAETGKAKKEGPLDVHTPNGTEPLKAKVTNGCNNLGIIMDHSPEPSFINP
  LSALQSIMNTHLGKVSKPVSPSLDPLAMLYKISNSMLDKPVYPATPVKQADAIDRYYYENSDQPIDLTKS
  KNKPLVSSVADSVASPLRESALMDISDMVKNLTGRLTPKSSTPSTVSEKSDADGSSFEEALDELSPVHKR
  KGRQSNWNPQHLLILQAQFASSLRETTEGKYIMSDLGPQERVHISKFTGLSMTTISHWLANVKYQLRRTG
  GTKFLKNLDTGHPVFFCNDCASQFRTASTYISHLETHLGFSLKDLSKLPLNQIQEQQNVSKVLTNKTLGP
  LGATEEDLGSTFQCKLCNRTFASKHAVKLHLSKTHGKSPEDHLIYVTELEKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005786
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005786.6
• RefSeq Size: 4982 bp
• RefSeq ORF: 3099 bp
• Locus ID: 10194
• UniProt ID: Q6ZSZ6
• Cytogenetics: 18q22.3
• Domains: homeobox, zf-C2H2
• Protein Families: Transcription Factors
• MW: 112.5 kDa
• Gene Summary: This gene encodes a colon cancer antigen that was defined by serological analysis of recombinant cDNA expression libraries. The encoded protein is a member of the teashirt C2H2-type zinc-finger protein family and may be involved in transcriptional regulation of developmental processes. Mutations in this gene may be associated with congenital aural atresia syndrome. [provided by RefSeq, Jan 2012]