Glyt1 (SLC6A9) (NM_006934) Human Tagged ORF Clone

CAT#: RC215895

SLC6A9 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, glycine), member 9 (SLC6A9), transcript variant 1

Product Images

Circular map for RC215895

• 

  Western blot validation of overexpression lysate (Cat# LY416313) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215895 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glyt1
• Synonyms: GCENSG; GLYT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215895 representing NM_006934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGAGCGGCGGAGACACGCGGGCTGCGATCGCTCGCCCCAGGATGGCCGCGGCTCATGGACCTGTGGCCC
  CCTCTTCCCCAGAACAGAATGGTGCTGTGCCCAGCGAGGCCACCAAGAGGGACCAGAACCTCAAACGGGG
  CAACTGGGGCAACCAGATCGAGTTTGTACTGACGAGCGTGGGCTATGCCGTGGGCCTGGGCAATGTCTGG
  CGCTTCCCATACCTCTGCTATCGCAACGGGGGAGGCGCCTTCATGTTCCCCTACTTCATCATGCTCATCT
  TCTGCGGGATCCCCCTCTTCTTCATGGAGCTCTCCTTCGGCCAGTTTGCAAGCCAGGGGTGCCTGGGGGT
  CTGGAGGATCAGCCCCATGTTCAAAGGAGTGGGCTATGGTATGATGGTGGTGTCCACCTACATCGGCATC
  TACTACAATGTGGTCATCTGCATCGCCTTCTACTACTTCTTCTCGTCCATGACGCACGTGCTGCCCTGGG
  CCTACTGCAATAACCCCTGGAACACGCATGACTGCGCCGGTGTACTGGACGCCTCCAACCTCACCAATGG
  CTCTCGGCCAGCCGCCTTGCCCAGCAACCTCTCCCACCTGCTCAACCACAGCCTCCAGAGGACCAGCCCC
  AGCGAGGAGTACTGGAGGCTGTACGTGCTGAAGCTGTCAGATGACATTGGGAACTTTGGGGAGGTGCGGC
  TGCCCCTCCTTGGCTGCCTCGGTGTCTCCTGGTTGGTCGTCTTCCTCTGCCTCATCCGAGGGGTCAAGTC
  TTCAGGGAAAGTGGTGTACTTCACGGCCACGTTCCCCTACGTGGTGCTGACCATTCTGTTTGTCCGCGGA
  GTGACCCTGGAGGGAGCCTTTGACGGCATCATGTACTACCTAACCCCGCAGTGGGACAAGATCCTGGAGG
  CCAAGGTGTGGGGTGATGCTGCCTCCCAGATCTTCTACTCACTGGGCTGCGCGTGGGGAGGCCTCATCAC
  CATGGCTTCCTACAACAAGTTCCACAATAACTGTTACCGGGACAGTGTCATCATCAGCATCACCAACTGT
  GCCACCAGCGTCTATGCTGGCTTCGTCATCTTCTCCATCCTCGGCTTCATGGCCAATCACCTGGGCGTGG
  ATGTGTCCCGTGTGGCAGACCACGGCCCTGGCCTGGCCTTCGTGGCTTACCCCGAGGCCCTCACACTACT
  TCCCATCTCCCCGCTGTGGTCTCTGCTCTTCTTCTTCATGCTTATCCTGCTGGGGCTGGGCACTCAGTTC
  TGCCTCCTGGAGACGCTGGTCACAGCCATTGTGGATGAGGTGGGGAATGAGTGGATCCTGCAGAAAAAGA
  CCTATGTGACCTTGGGCGTGGCTGTGGCTGGCTTCCTGCTGGGCATCCCCCTCACCAGCCAGGCAGGCAT
  CTATTGGCTGCTGCTGATGGACAACTATGCGGCCAGCTTCTCCTTGGTGGTCATCTCCTGCATCATGTGT
  GTGGCCATCATGTACATCTACGGGCACCGGAACTACTTCCAGGACATCCAGATGATGCTGGGATTCCCAC
  CACCCCTCTTCTTTCAGATCTGCTGGCGCTTCGTCTCTCCCGCCATCATCTTCTTTATTCTAGTTTTCAC
  TGTGATCCAGTACCAGCCGATCACCTACAACCACTACCAGTACCCAGGCTGGGCCGTGGCCATTGGCTTC
  CTCATGGCTCTGTCCTCCGTCCTCTGCATCCCCCTCTACGCCATGTTCCGGCTCTGCCGCACAGACGGGG
  ACACCCTCCTCCAGCGTTTGAAAAATGCCACAAAGCCAAGCAGAGACTGGGGCCCTGCCCTCCTGGAGCA
  CCGGACAGGGCGCTACGCCCCCACCATAGCCCCCTCTCCTGAGGACGGCTTCGAGGTCCAGCCACTGCAC
  CCGGACAAGGCGCAGATCCCCATTGTGGGCAGTAATGGCTCCAGCCGCCTCCAGGACTCCCGGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215895 representing NM_006934
  Red=Cloning site Green=Tags(s)
  
  MSGGDTRAAIARPRMAAAHGPVAPSSPEQNGAVPSEATKRDQNLKRGNWGNQIEFVLTSVGYAVGLGNVW
  RFPYLCYRNGGGAFMFPYFIMLIFCGIPLFFMELSFGQFASQGCLGVWRISPMFKGVGYGMMVVSTYIGI
  YYNVVICIAFYYFFSSMTHVLPWAYCNNPWNTHDCAGVLDASNLTNGSRPAALPSNLSHLLNHSLQRTSP
  SEEYWRLYVLKLSDDIGNFGEVRLPLLGCLGVSWLVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFVRG
  VTLEGAFDGIMYYLTPQWDKILEAKVWGDAASQIFYSLGCAWGGLITMASYNKFHNNCYRDSVIISITNC
  ATSVYAGFVIFSILGFMANHLGVDVSRVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQF
  CLLETLVTAIVDEVGNEWILQKKTYVTLGVAVAGFLLGIPLTSQAGIYWLLLMDNYAASFSLVVISCIMC
  VAIMYIYGHRNYFQDIQMMLGFPPPLFFQICWRFVSPAIIFFILVFTVIQYQPITYNHYQYPGWAVAIGF
  LMALSSVLCIPLYAMFRLCRTDGDTLLQRLKNATKPSRDWGPALLEHRTGRYAPTIAPSPEDGFEVQPLH
  PDKAQIPIVGSNGSSRLQDSRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006934
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006934.4
• RefSeq Size: 3213 bp
• RefSeq ORF: 1959 bp
• Locus ID: 6536
• UniProt ID: P48067
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 72.3 kDa
• Gene Summary: The amino acid glycine acts as an inhibitory neurotransmitter in the central nervous system. The protein encoded by this gene is one of two transporters that stop glycine signaling by removing it from the synaptic cleft. [provided by RefSeq, Jun 2016]