PRAMEF8 (NM_001012276) Human Tagged ORF Clone

CAT#: RC215812

PRAMEF8 (Myc-DDK-tagged)-Human PRAME family member 8 (PRAMEF8)

Product Images

Circular map for RC215812

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  Western blot validation of overexpression lysate (Cat# LY423316) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215812 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRAMEF8
• Synonyms: PRAMEF24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215812 representing NM_001012276
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATCAGGGCCCCACCCAGACTCCTGGAGCTGGCAAGGCAGAGGCTGCTGAGGGACCAGGCCTTGG
  CCATCTCCACCATGGAGGAGCTGCCCAGGGAGCTCTTCCCCACACTGTTCATGGAGGCCTTCAGCAGGAG
  ACGCTGTGAAACCCTGAAAACAATGGTGCAGGCCTGGCCTTTCACCCGCCTCCCTCTAGGGTCCCTGATG
  AAGTCGCCTCATCTGGAGTCATTAAAATCTGTGCTGGAAGGGGTTGATGTGCTGTTGACCCAAGAGGTTC
  GCCCCAGGCAGTCAAAACTTCAAGTGCTGGACTTGAGGAATGTGGATGAGAACTTCTGCGACATATTTTC
  TGGAGCTACTGCATCCTTCCCGGAGGCTCTGAGTCAGAAGCAAACAGCAGATAACTGTCCAGGGACAGGC
  AGGCAGCAGCCATTCATGGTGTTCATAGACCTTTGTCTCAAGAACAGGACACTAGATGAATGCCTCACCC
  ACCTCTTAGAGTGGGGCAAGCAGAGAAAAGGCTTACTGCATGTGTGTTGCCAGGAGCTGCAGGTTTTTGG
  AATGCCCATCCACAGTATCATAGAGGTCCTGAACATGGTGGAGCTTGACTGTATCCAGGAGGTGGAAGTG
  TGCTGCCCCTGGGAGCTGTCCACTCTTGTGAAGTTTGCCCCTTACCTGGGCCAGATGAGGAATCTCCGCA
  AACTTGTTCTCTTCAACATCCGTGCATCTGCCTGCATTCCCCCAGACAACAAGGGGCAGTTCATTGCCCG
  ATTCACCTCTCAGTTCCTCAAGCTGGACTATTTCCAGAATCTGTCTATGCACTCCGTCTCTTTCCTCGAA
  GGCCACCTGGACCAGCTGCTCAGGTGTCTCCAGGCCTCCTTGGAGATGGTCGTTATGACCGACTGCCTGC
  TGTCAGAGTCGGACTTGAAGCATCTCTCTTGGTGCCCGAGCATCCGTCAATTAAAGGAGCTGGACCTGAG
  GGGTGTCACACTGACCCATTTCAGCCCTGAGCCCCTCACAGGTCTGCTGGAGCAAGTTGTGGCCACCCTG
  CAGACCCTGGACTTAGAGGACTGTGGGATCATGGATTCCCAACTCAGCGCCATCCTGCCTGTCCTGAGCC
  GCTGCTCCCAGCTCAGCACCTTCAGCTTCTGTGGGAACCTCATCTCCATGGCTGCCCTTGAGAACCTGCT
  GCGCCACACCGTCGGGCTGAGCAAGCTAAGCCTGGAGCTGTATCCTGCCCCTCTGGAGAGTTATGACACC
  CAGGGTGCTCTCTGCTGGGGGAGATTTGCTGAACTTGGGGCTGAGCTGATGAAGACACTGAGGGACTTAA
  GGCAGCCCAAGATCATTGTGTTCTGCACCGTCCCCTGCCCTCGCTGTGGCATCAGGGCCTCCTATGACCT
  GGAGCCCAGTCACTGCCTCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215812 representing NM_001012276
  Red=Cloning site Green=Tags(s)
  
  MSIRAPPRLLELARQRLLRDQALAISTMEELPRELFPTLFMEAFSRRRCETLKTMVQAWPFTRLPLGSLM
  KSPHLESLKSVLEGVDVLLTQEVRPRQSKLQVLDLRNVDENFCDIFSGATASFPEALSQKQTADNCPGTG
  RQQPFMVFIDLCLKNRTLDECLTHLLEWGKQRKGLLHVCCQELQVFGMPIHSIIEVLNMVELDCIQEVEV
  CCPWELSTLVKFAPYLGQMRNLRKLVLFNIRASACIPPDNKGQFIARFTSQFLKLDYFQNLSMHSVSFLE
  GHLDQLLRCLQASLEMVVMTDCLLSESDLKHLSWCPSIRQLKELDLRGVTLTHFSPEPLTGLLEQVVATL
  QTLDLEDCGIMDSQLSAILPVLSRCSQLSTFSFCGNLISMAALENLLRHTVGLSKLSLELYPAPLESYDT
  QGALCWGRFAELGAELMKTLRDLRQPKIIVFCTVPCPRCGIRASYDLEPSHCLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012276
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012276.2
• RefSeq Size: 1844 bp
• RefSeq ORF: 1425 bp
• Locus ID: 391002
• UniProt ID: Q5VWM4
• Cytogenetics: 1p36.21
• MW: 53.5 kDa