AP2B1 (NM_001282) Human Tagged ORF Clone

CAT#: RC215791

AP2B1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 2

Product Images

Circular map for RC215791

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  Western blot validation of overexpression lysate (Cat# LY420031) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215791 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AP2B1 protein (Cat# TP315791). The protein was produced from HEK293T cells transfected with AP2B1 cDNA clone (Cat# RC215791) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP2B1
• Synonyms: ADTB2; AP2-BETA; AP105B; CLAPB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215791 representing NM_001282
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACTCCAAGTATTTCACAACCAATAAAAAAGGAGAAATATTTGAACTAAAAGCTGAACTCAACA
  ATGAAAAGAAAGAAAAGAGAAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGATGT
  TAGTTCTCTCTTTCCAGACGTAGTGAACTGTATGCAGACTGACAATCTGGAACTAAAGAAGCTTGTGTAT
  CTCTACTTGATGAACTACGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTAAACAGCTTTGTGAAGG
  ACTGTGAAGATCCTAATCCTTTGATTCGAGCCTTGGCAGTCAGAACCATGGGGTGCATCCGGGTAGACAA
  AATTACAGAATATCTCTGTGAGCCGCTCCGCAAGTGCTTGAAGGATGAGGATCCCTATGTTCGGAAAACA
  GCAGCAGTCTGCGTGGCAAAACTCCATGATATCAATGCCCAAATGGTGGAAGATCAGGGATTTCTGGATT
  CTCTACGGGATCTCATAGCAGATTCAAATCCAATGGTGGTGGCTAATGCCGTAGCGGCATTATCTGAAAT
  CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCACAGAACATTAATAAGCTGCTGACAGCC
  CTGAATGAATGCACTGAATGGGGCCAGATTTTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATG
  ATCGGGAGGCTCAGAGCATCTGTGAGCGGGTAACTCCCCGGCTATCCCATGCCAACTCAGCAGTGGTGCT
  TTCAGCGGTAAAAGTCCTAATGAAGTTTCTAGAATTGTTACCTAAGGATTCTGACTACTACAATATGCTG
  CTGAAGAAGTTAGCCCCTCCACTTGTCACTTTGCTGTCTGGGGAGCCAGAAGTGCAGTATGTCGCCCTGA
  GGAACATCAACTTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAAGTCTTCTTTGTGAA
  GTACAATGATCCCATCTATGTTAAACTAGAGAAGTTGGACATCATGATTCGTTTGGCATCTCAAGCCAAC
  ATTGCTCAGGTTCTGGCAGAACTGAAAGAATATGCTACAGAGGTGGATGTTGACTTTGTTCGAAAAGCTG
  TGCGGGCCATTGGACGGTGTGCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTAAGCACATTGCTTGA
  TCTAATCCAGACCAAAGTGAATTATGTGGTCCAAGAAGCAATTGTTGTCATCAGGGACATCTTCCGCAAA
  TACCCCAACAAGTATGAAAGTATCATCGCCACTCTGTGTGAGAACTTAGACTCGCTGGATGAGCCAGATG
  CTCGAGCAGCTATGATTTGGATTGTGGGAGAATATGCTGAAAGAATTGACAATGCAGATGAGTTACTAGA
  AAGCTTCCTGGAGGGTTTTCACGATGAAAGCACCCAGGTGCAGCTCACTCTGCTTACTGCCATAGTGAAG
  CTGTTTCTCAAGAAACCATCAGAAACACAGGAGCTAGTCCAGCAGGTCTTGAGTTTGGCAACACAGGATT
  CTGATAATCCTGACCTTCGAGACCGGGGCTATATTTATTGGCGCCTTCTCTCAACTGACCCTGTTACAGC
  TAAAGAAGTAGTCTTGTCTGAGAAGCCACTGATCTCTGAGGAGACGGACCTTATTGAGCCAACTCTGCTG
  GATGAGCTAATCTGCCACATTGGTTCTTTGGCCTCTGTGTATCATAAGCCTCCCAATGCTTTTGTGGAAG
  GAAGTCATGGAATTCATCGTAAACACTTGCCAATTCATCATGGGAGCACTGATGCAGGTGACAGCCCTGT
  TGGCACTACCACTGCAACGAACCTGGAACAGCCTCAGGTTATCCCCTCTCAAGGTGATCTTCTAGGGGAT
  CTTTTAAACCTTGACCTCGGTCCCCCAGTCAATGTGCCACAGGTGTCCTCCATGCAGATGGGAGCAGTGG
  ATCTCCTAGGAGGAGGACTAGATAGTCTGGTGGGACAATCCTTCATCCCATCATCGGTCCCTGCAACCTT
  TGCTCCTTCACCTACACCTGCTGTGGTCAGCAGTGGACTGAATGACCTGTTTGAACTCTCCACAGGGATA
  GGCATGGCACCTGGTGGATATGTGGCTCCTAAGGCTGTCTGGCTACCTGCAGTAAAGGCTAAAGGCTTGG
  AGATTTCCGGAACATTTACTCACCGCCAAGGGCACATCTATATGGAAATGAACTTCACCAATAAAGCTCT
  GCAGCACATGACAGATTTTGCAATCCAGTTTAACAAAAATAGCTTTGGTGTCATCCCCAGCACTCCTCTG
  GCCATCCATACACCACTGATGCCAAACCAGAGCATTGATGTCTCCCTGCCTCTCAATACCTTGGGCCCAG
  TCATGAAGATGGAACCTCTGAATAACCTCCAGGTGGCTGTGAAAAACAATATCGATGTCTTCTACTTCAG
  CTGCCTCATCCCACTCAATGTGCTTTTTGTAGAAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACA
  TGGAAGGATATTCCCAATGAAAATGAACTTCAGTTTCAGATTAAGGAATGTCATTTAAATGCTGACACTG
  TTTCCAGCAAGTTGCAAAACAACAATGTTTATACTATTGCCAAGAGGAATGTGGAAGGGCAGGACATGCT
  GTACCAATCCCTGAAGCTCACTAATGGCATTTGGATTTTGGCCGAACTACGTATCCAGCCAGGAAACCCC
  AATTACACGCTGTCACTGAAGTGTAGAGCTCCTGAAGTCTCTCAATACATCTATCAGGTCTACGACAGCA
  TTTTGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215791 representing NM_001282
  Red=Cloning site Green=Tags(s)
  
  MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY
  LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT
  AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA
  LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML
  LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN
  IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK
  YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK
  LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL
  DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGD
  LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLVGQSFIPSSVPATFAPSPTPAVVSSGLNDLFELSTGI
  GMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPL
  AIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLAT
  WKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNP
  NYTLSLKCRAPEVSQYIYQVYDSILKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282
• ORF Size: 2811 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282.3
• RefSeq Size: 5730 bp
• RefSeq ORF: 2814 bp
• Locus ID: 163
• UniProt ID: P63010
• Cytogenetics: 17q12
• Domains: Armadillo_seg, Adaptin_N, Alpha_adaptinC2
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease
• MW: 104.4 kDa
• Gene Summary: The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]