C3orf37 (HMCES) (NM_020187) Human Tagged ORF Clone

CAT#: RC215567

C3orf37 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 2

Product Images

Circular map for RC215567

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  Western blot validation of overexpression lysate (Cat# LY412609) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215567 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HMCES protein (Cat# TP315567). The protein was produced from HEK293T cells transfected with HMCES cDNA clone (Cat# RC215567) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C3orf37
• Synonyms: C3orf37; DC12; SRAPD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215567 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGGGCGAACATCCTGTCACTTACCTAGAGATGTTCTCACGAGAGCTTGCGCCTACCAGGATCGGC
  GGGGCCAGCAGCGGCTCCCGGAGTGGAGGGACCCTGATAAGTACTGCCCCTCTTACAACAAGAGTCCTCA
  ATCCAACAGCCCAGTGCTTCTGTCTCGACTGCACTTTGAGAAGGATGCAGACTCATCTGAGCGTATCATT
  GCTCCCATGCGCTGGGGCTTGGTCCCTTCTTGGTTCAAAGAAAGTGATCCTTCCAAGCTGCAGTTCAATA
  CTACCAACTGTCGTAGTGATACCGTAATGGAGAAACGGTCATTTAAGGTGCCTCTGGGAAAGGGAAGACG
  CTGTGTCGTTTTAGCAGATGGATTCTATGAGTGGCAGCGATGTCAGGGAACAAACCAGAGGCAGCCATAC
  TTCATCTATTTTCCTCAAATCAAGACAGAGAAGTCAGGTAGCATTGGTGCTGCAGATAGTCCTGAGAACT
  GGGAGAAAGTCTGGGACAACTGGAGGCTGCTGACAATGGCCGGGATCTTTGACTGCTGGGAGCCCCCAGA
  GGGAGGAGATGTCCTGTATTCCTATACCATCATCACAGTGGATTCCTGCAAAGGCTTGAGTGACATCCAC
  CACAGGATGCCTGCCATATTAGATGGAGAGGAGGCAGTTTCTAAATGGCTTGACTTTGGTGAAGTCTCAA
  CTCAGGAAGCTCTGAAATTAATCCACCCAACAGAGAACATCACCTTCCATGCAGTCTCTTCTGTGGTGAA
  CAACTCGCGAAACAACACTCCTGAGTGTCTGGCTCCTGTCGACTTGGTGGTCAAAAAGGAGCTCAGGGCA
  AGTGGCAGTAGCCAGAGGATGTTGCAGTGGTTGGCCACAAAGTCACCCAAAAAGGAAGACTCAAAAACAC
  CTCAAAAGGAAGAGTCAGATGTTCCCCAGTGGTCCAGTCAGTTCCTGCAGAAGAGTCCACTCCCCACCAA
  GAGAGGCACTGCAGGACTCCTAGAGCAATGGCTGAAGCGGGAGAAGGAGGAGGAACCTGTGGCCAAGCGT
  CCTTACAGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215567 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCGRTSCHLPRDVLTRACAYQDRRGQQRLPEWRDPDKYCPSYNKSPQSNSPVLLSRLHFEKDADSSERII
  APMRWGLVPSWFKESDPSKLQFNTTNCRSDTVMEKRSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPY
  FIYFPQIKTEKSGSIGAADSPENWEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIH
  HRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVDLVVKKELRA
  SGSSQRMLQWLATKSPKKEDSKTPQKEESDVPQWSSQFLQKSPLPTKRGTAGLLEQWLKREKEEEPVAKR
  PYSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020187
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020187.3
• RefSeq Size: 1638 bp
• RefSeq ORF: 1065 bp
• Locus ID: 56941
• UniProt ID: Q96FZ2
• Cytogenetics: 3q21.3
• Domains: DUF159
• MW: 40.6 kDa
• Gene Summary: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA (PubMed:30554877). The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells (By similarity). May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (By similarity).[UniProtKB/Swiss-Prot Function]