H1oo (H1FOO) (NM_153833) Human Tagged ORF Clone

CAT#: RC215554

H1FOO (Myc-DDK-tagged)-Human H1 histone family, member O, oocyte-specific (H1FOO)

Product Images

Circular map for RC215554

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  Western blot validation of overexpression lysate (Cat# LY406918) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215554 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified H1FOO protein (Cat# TP315554). The protein was produced from HEK293T cells transfected with H1FOO cDNA clone (Cat# RC215554) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H1oo
• Synonyms: H1.8; H1FOO; H1oo; osH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215554 representing NM_153833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTGGGAGCGTCACCAGCGACATCTCACCCTCCTCGACTTCCACAGCAGGATCATCCAGGTCTC
  CTGAATCTGAAAAGCCAGGCCCGAGCCACGGCGGTGTCCCACCAGGAGGCCCGAGCCACAGCAGCCTCCC
  GGTGGGACGCCGCCACCCCCCGGTGCTACGCATGGTGCTGGAGGCGCTGCAGGCTGGGGAGCAGCGCCGG
  GGCACGTCGGTGGCAGCTATCAAGCTCTACATCCTGCACAAGTACCCAACAGTGGACGTCCTCCGCTTCA
  AGTACCTGCTGAAGCAGGCGCTGGCCACTGGCATGCGCCGTGGCCTCCTCGCCAGGCCCCTCAACTCCAA
  AGCCAGGGGGGCCACTGGCAGCTTCAAATTAGTTCCCAAGCACAAGAAGAAAATCCAGCCCAGGAAGATG
  GCCCCCGCGACGGCTCCCAGGAGAGCGGGTGAGGCCAAGGGGAAGGGCCCCAAGAAACCAAGTGAGGCCA
  AGGAGGACCCTCCCAACGTGGGCAAGGTGAAAAAGGCAGCCAAGAGGCCAGCAAAGGTGCAGAAGCCTCC
  TCCCAAGCCAGGCGCAGCCACAGAGAAGGCTCGCAAGCAAGGCGGCGCGGCCAAGGACACCAGGGCACAG
  TCGGGAGAGGCTAGGAAGGTGCCCCCCAAGCCAGACAAGGCCATGCGGGCACCTTCCAGTGCTGGTGGGC
  TCAGCAGGAAGGCAAAGGCCAAAGGCAGCAGGAGCAGCCAAGGAGATGCTGAGGCCTACAGGAAAACCAA
  AGCTGAGAGTAAGAGTTCAAAACCCACGGCCAGCAAGGTCAAGAATGGTGCTGCTTCCCCGACCAAAAAG
  AAGGTGGTGGCCAAGGCCAAGGCCCCTAAAGCTGGGCAGGGGCCAAACACCAAGGCTGCTGCTCCTGCTA
  AGGGCAGTGGGTCCAAGGTGGTACCTGCACATTTGTCCAGGAAGACAGAGGCCCCCAAGGGCCCTAGAAA
  GGCTGGGCTGCCCATCAAGGCCTCATCATCCAAAGTGTCCAGCCAGAGGGCTGAAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215554 representing NM_153833
  Red=Cloning site Green=Tags(s)
  
  MAPGSVTSDISPSSTSTAGSSRSPESEKPGPSHGGVPPGGPSHSSLPVGRRHPPVLRMVLEALQAGEQRR
  GTSVAAIKLYILHKYPTVDVLRFKYLLKQALATGMRRGLLARPLNSKARGATGSFKLVPKHKKKIQPRKM
  APATAPRRAGEAKGKGPKKPSEAKEDPPNVGKVKKAAKRPAKVQKPPPKPGAATEKARKQGGAAKDTRAQ
  SGEARKVPPKPDKAMRAPSSAGGLSRKAKAKGSRSSQGDAEAYRKTKAESKSSKPTASKVKNGAASPTKK
  KVVAKAKAPKAGQGPNTKAAAPAKGSGSKVVPAHLSRKTEAPKGPRKAGLPIKASSSKVSSQRAEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153833
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153833.3
• RefSeq Size: 1067 bp
• RefSeq ORF: 1041 bp
• Locus ID: 132243
• UniProt ID: Q8IZA3
• Cytogenetics: 3q22.1
• MW: 35.6 kDa
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Oct 2015]