LIPN (NM_001102469) Human Tagged ORF Clone

CAT#: RC215417

LIPN (Myc-DDK-tagged)-Human lipase, family member N (LIPN)

Product Images

Circular map for RC215417

• 

  Western blot validation of overexpression lysate (Cat# LY420154) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215417 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPN
• Synonyms: ARCI8; bA186O14.3; LI4; LIPL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215417 representing NM_001102469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGCTGCTTTTAACAACAACTTGTTTGATCTGTGGAACTTTAAATGCTGGTGGATTCCTTGATT
  TGGAAAATGAAGTGAATCCTGAGGTGTGGATGAATACTAGTGAAATCATCATCTACAATGGCTACCCCAG
  TGAAGAGTATGAAGTCACCACTGAAGATGGGTATATACTCCTTGTCAACAGAATTCCTTATGGGCGAACA
  CATGCTAGGAGCACAGGTCCCCGGCCAGTTGTGTATATGCAGCATGCCCTGTTTGCAGACAATGCCTACT
  GGCTTGAGAATTATGCTAATGGAAGCCTTGGATTCCTTCTAGCAGATGCAGGTTATGATGTATGGATGGG
  AAACAGTCGGGGAAACACTTGGTCAAGAAGACACAAAACACTCTCAGAGACAGATGAGAAATTCTGGGCC
  TTTAGTTTTGATGAAATGGCCAAATATGATCTCCCAGGAGTAATAGACTTCATTGTAAATAAAACTGGTC
  AGGAGAAATTGTATTTCATTGGACATTCACTTGGCACTACAATAGGGTTTGTAGCCTTTTCCACCATGCC
  TGAACTGGCACAAAGAATCAAAATGAATTTTGCCTTGGGTCCTACGATCTCATTCAAATATCCCACGGGC
  ATTTTTACCAGGTTTTTTCTACTTCCAAATTCCATAATCAAGGCTGTTTTTGGTACCAAAGGTTTCTTTT
  TAGAAGATAAGAAAACGAAGATAGCTTCTACCAAAATCTGCAACAATAAGATACTCTGGTTGATATGTAG
  CGAATTTATGTCCTTATGGGCTGGATCCAACAAGAAAAATATGAATCAGAGTCGAATGGATGTGTATATG
  TCACATGCTCCCACTGGTTCATCAGTACACAACATTCTGCATATAAAACAGCTTTACCACTCTGATGAAT
  TCAGAGCTTATGACTGGGGAAATGACGCTGATAATATGAAACATTACAATCAGAGTCATCCCCCTATATA
  TGACCTGACTGCCATGAAAGTGCCTACTGCTATTTGGGCTGGTGGACATGATGTCCTCGTAACACCCCAG
  GATGTGGCCAGGATACTCCCTCAAATCAAGAGTCTTCATTACTTTAAGCTATTGCCAGATTGGAACCACT
  TTGATTTTGTCTGGGGCCTCGATGCCCCTCAACGGATGTACAGTGAAATCATAGCTTTAATGAAGGCATA
  TTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215417 representing NM_001102469
  Red=Cloning site Green=Tags(s)
  
  MMWLLLTTTCLICGTLNAGGFLDLENEVNPEVWMNTSEIIIYNGYPSEEYEVTTEDGYILLVNRIPYGRT
  HARSTGPRPVVYMQHALFADNAYWLENYANGSLGFLLADAGYDVWMGNSRGNTWSRRHKTLSETDEKFWA
  FSFDEMAKYDLPGVIDFIVNKTGQEKLYFIGHSLGTTIGFVAFSTMPELAQRIKMNFALGPTISFKYPTG
  IFTRFFLLPNSIIKAVFGTKGFFLEDKKTKIASTKICNNKILWLICSEFMSLWAGSNKKNMNQSRMDVYM
  SHAPTGSSVHNILHIKQLYHSDEFRAYDWGNDADNMKHYNQSHPPIYDLTAMKVPTAIWAGGHDVLVTPQ
  DVARILPQIKSLHYFKLLPDWNHFDFVWGLDAPQRMYSEIIALMKAYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102469
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102469.2
• RefSeq Size: 1197 bp
• RefSeq ORF: 1197 bp
• Locus ID: 643418
• UniProt ID: Q5VXI9
• Cytogenetics: 10q23.31
• MW: 45.4 kDa
• Gene Summary: The gene encodes a lipase that is highly expressed in granular keratinocytes in the epidermis, and plays a role in the differentiation of keratinocytes. Mutations in this gene are associated with lamellar ichthyosis type 4. [provided by RefSeq, Dec 2011]