FUBP3 (NM_003934) Human Tagged ORF Clone

CAT#: RC215402

FUBP3 (Myc-DDK-tagged)-Human far upstream element (FUSE) binding protein 3 (FUBP3)

Product Images

Circular map for RC215402

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FUBP3 (Cat# RC215402, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FUBP3 antibody (Cat# TA812228). Positive lysates LY418344 (100ug) and LC418344 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418344) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215402 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FUBP3 protein (Cat# TP315402). The protein was produced from HEK293T cells transfected with FUBP3 cDNA clone (Cat# RC215402) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FUBP3
• Synonyms: FBP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215402 representing NM_003934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGGTGCAGGGGCAGAGCGCTCCTGTGGGGATGAAGGCCGAGGGCTTCGTGGATGCCCTGC
  ACCGGGTCCGGCAGATTGCTGCTAAAATTGATTCAATTCCTCACTTGAATAATTCCACACCTCTAGTGGA
  CCCCTCAGTATATGGATACGGAGTACAAAAACGGCCCTTGGATGATGGAGTAGGTAACCAGTTAGGGGCC
  TTGGTACATCAAAGGACGGTAATAACGGAAGAATTCAAAGTGCCTGACAAAATGGTTGGATTTATTATCG
  GCAGGGGAGGTGAGCAGATTTCACGGATTCAAGCAGAATCTGGTTGCAAAATTCAGATTGCTTCAGAGAG
  TTCTGGGATTCCAGAGAGGCCCTGTGTACTTACCGGAACCCCAGAAAGTATTGAACAAGCCAAACGGCTC
  CTGGGACAGATTGTGGACCGCTGTCGAAATGGACCTGGCTTTCATAATGACATAGACAGCAACAGCACAA
  TCCAGGAGATTCTCATTCCCGCATCTAAAGTGGGTCTGGTCATCGGCAGAGGAGGGGAAACAATCAAGCA
  GTTGCAGGAGCGGACAGGGGTGAAGATGGTCATGATCCAGGATGGCCCATTGCCCACGGGAGCAGACAAG
  CCTCTTCGTATCACTGGAGATGCATTTAAAGTACAGCAAGCAAGAGAAATGGTACTAGAGATTATCCGAG
  AAAAAGACCAAGCTGACTTTCGGGGTGTACGCGGCGATTTCAACTCTCGAATGGGAGGAGGCAGTATAGA
  GGTATCTGTGCCTAGGTTTGCTGTGGGGATTGTAATAGGAAGAAACGGGGAAATGATCAAAAAGATCCAG
  AATGATGCTGGTGTGAGGATTCAGTTTAAACCAGATGATGGGATTAGTCCAGAAAGAGCTGCCCAGGTCA
  TGGGCCCTCCGGATCGGTGTCAGCATGCAGCGCATATCATCAGCGAGCTGATTCTTACAGCCCAGGAAAG
  AGACGGCTTTGGAGGCCTGGCAGCAGCCAGAGGAAGAGGTCGTGGCCGTGGCGACTGGAGCGTGGGAGCC
  CCTGGTGGCGTCCAGGAGATAACATACACGGTGCCAGCCGATAAGTGTGGCCTCGTCATAGGCAAAGGGG
  GTGAGAACATCAAAAGCATCAACCAGCAGTCAGGGGCGCACGTGGAGCTTCAGAGGAACCCCCCTCCCAA
  CAGCGACCCCAACCTGCGGAGATTCACCATCAGGGGGGTTCCCCAGCAGATCGAGGTGGCCAGGCAGCTC
  ATAGATGAGAAAGTTGGCGGGACCAATCTCGGAGCACCTGGAGCCTTCGGACAGAGTCCATTCAGCCAGC
  CACCTGCCCCACCTCATCAAAATACCTTTCCTCCAAGGAGCTCCGGGTGCTTCCCAAACATGGCTGCCAA
  GGTGAATGGGAACCCCCACAGCACCCCTGTGAGTGGTCCTCCGGCCTTTCTGACCCAGGGCTGGGGCAGC
  ACCTACCAGGCGTGGCAGCAGCCCACACAGCAGGTCCCAAGCCAGCAGAGCCAGCCGCAGAGCAGCCAGC
  CCAACTACAGCAAGGCCTGGGAAGACTATTACAAAAAACAGAGTCACGCCGCCAGCGCTGCTCCTCAGGC
  CAGCTCCCCACCGGACTACACAATGGCCTGGGCAGAATATTACAGACAGCAGGTCGCTTTCTACGGACAG
  ACGTTAGGGCAGGCGCAGGCCCACAGCCAGGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215402 representing NM_003934
  Red=Cloning site Green=Tags(s)
  
  MAELVQGQSAPVGMKAEGFVDALHRVRQIAAKIDSIPHLNNSTPLVDPSVYGYGVQKRPLDDGVGNQLGA
  LVHQRTVITEEFKVPDKMVGFIIGRGGEQISRIQAESGCKIQIASESSGIPERPCVLTGTPESIEQAKRL
  LGQIVDRCRNGPGFHNDIDSNSTIQEILIPASKVGLVIGRGGETIKQLQERTGVKMVMIQDGPLPTGADK
  PLRITGDAFKVQQAREMVLEIIREKDQADFRGVRGDFNSRMGGGSIEVSVPRFAVGIVIGRNGEMIKKIQ
  NDAGVRIQFKPDDGISPERAAQVMGPPDRCQHAAHIISELILTAQERDGFGGLAAARGRGRGRGDWSVGA
  PGGVQEITYTVPADKCGLVIGKGGENIKSINQQSGAHVELQRNPPPNSDPNLRRFTIRGVPQQIEVARQL
  IDEKVGGTNLGAPGAFGQSPFSQPPAPPHQNTFPPRSSGCFPNMAAKVNGNPHSTPVSGPPAFLTQGWGS
  TYQAWQQPTQQVPSQQSQPQSSQPNYSKAWEDYYKKQSHAASAAPQASSPPDYTMAWAEYYRQQVAFYGQ
  TLGQAQAHSQEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003934
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003934.2
• RefSeq Size: 3175 bp
• RefSeq ORF: 1719 bp
• Locus ID: 8939
• UniProt ID: Q96I24
• Cytogenetics: 9q34.11-q34.12
• MW: 61.5 kDa
• Gene Summary: May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.[UniProtKB/Swiss-Prot Function]