RECQ4 (RECQL4) (NM_004260) Human Tagged ORF Clone

CAT#: RC215399

RECQL4 (Myc-DDK-tagged)-Human RecQ protein-like 4 (RECQL4)

Product Images

Circular map for RC215399

• 

  Western blot validation of overexpression lysate (Cat# LY418114) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215399 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RECQ4
• Synonyms: RECQ4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215399 representing NM_004260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCTGCGGGACGTGCGGGAGCGGCTGCAGGCGTGGGAGCGCGCGTTCCGACGGCAGCGCGGGC
  GGCGACCGAGCCAGGACGACGTGGAGGCGGCGCCGGAGGAGACCCGCGCGCTCTACCGGGAATACCGCAC
  TCTGAAGCGTACCACGGGCCAGGCCGGCGGCGGGCTCCGCAGCTCCGAGTCGCTCCCCGCGGCGGCCGAA
  GAGGCGCCAGAGCCCCGCTGCTGGGGGCCCCATCTGAATCGGGCTGCGACCAAGAGTCCACAGTCTACGC
  CAGGGCGGAGCCGCCAGGGCTCGGTGCCGGACTACGGGCAGCGGCTCAAGGCCAATCTGAAAGGCACCCT
  GCAGGCCGGACCAGCCCTGGGCCGCAGACCGTGGCCTCTAGGAAGAGCCTCATCTAAGGCATCCACCCCA
  AAGCCCCCAGGTACAGGGCCTGTCCCCTCCTTTGCAGAAAAAGTCAGTGATGAGCCTCCACAGCTCCCTG
  AGCCCCAGCCAAGGCCAGGCCGGCTCCAGCATCTGCAGGCATCCCTGAGCCAGCGGCTGGGCTCCCTAGA
  TCCTGGCTGGTTACAGCGATGTCACAGTGAGGTCCCAGATTTTCTGGGGGCCCCCAAAGCCTGCAGGCCT
  GATCTAGGCTCAGAGGAATCACAACTTCTGATCCCTGGTGAGTCGGCTGTCCTTGGTCCTGGTGCTGGCT
  CCCAGGGCCCAGAGGCTTCAGCCTTCCAAGAAGTCAGCATCCGTGTGGGGAGCCCCCAGCCCAGCAGCAG
  TGGAGGCGAGAAGCGGAGATGGAACGAGGAGCCCTGGGAGAGCCCCGCACAGGTCCAGCAGGAGAGCAGC
  CAAGCTGGACCCCCATCGGAGGGGGCTGGGGCTGTAGCAGTTGAGGAAGACCCTCCAGGGGAACCTGTAC
  AGGCACAGCCACCTCAGCCCTGCAGCAGCCCATCGAACCCCAGGTACCACGGACTCAGCCCCTCCAGTCA
  AGCTAGGGCTGGGAAGGCTGAGGGCACAGCCCCCCTGCACATCTTCCCTCGGCTGGCCCGCCATGACAGG
  GGCAATTACGTACGGCTCAACATGAAGCAGAAACACTACGTGCGGGGCCGGGCACTCCGTAGCAGGCTCC
  TCCGCAAGCAGGCATGGAAGCAGAAGTGGCGGAAGAAAGGGGAGTGTTTTGGGGGTGGTGGTGCCACAGT
  CACAACCAAGGAGTCTTGTTTCCTGAACGAGCAGTTCGATCACTGGGCAGCCCAGTGTCCCCGGCCAGCA
  AGTGAGGAAGACACAGATGCTGTTGGGCCTGAGCCACTGGTTCCTTCACCACAACCTGTACCTGAGGTGC
  CCAGCCTGGACCCCACCGTGCTGCCACTCTACTCCCTGGGGCCCTCAGGGCAGTTGGCAGAGACGCCGGC
  TGAGGTGTTCCAGGCCCTGGAGCAGCTGGGGCACCAAGCCTTTCGCCCTGGGCAGGAGCGTGCAGTCATG
  CGGATCCTGTCTGGCATCTCCACGCTGCTGGTGCTGCCTACAGGTGCCGGCAAGTCCCTGTGCTACCAGC
  TCCCAGCGCTGCTCTACAGCCGGCGCAGCCCCTGCCTCACGTTGGTCGTCTCTCCCCTGCTGTCACTCAT
  GGATGACCAGGTGTCTGGCCTGCCACCGTGTCTCAAGGCGGCCTGCATACACTCGGGCATGACCAGGAAG
  CAACGGGAATCTGTCCTGCAGAAGATTCGGGCAGCCCAGGTACACGTGCTGATGCTGACACCTGAGGCAC
  TGGTGGGGGCGGGAGGCCTCCCTCCAGCCGCACAGCTGCCTCCAGTTGCTTTTGCCTGCATTGATGAGGC
  CCACTGCCTCTCCCAGTGGTCCCACAACTTCCGGCCCTGCTACCTGCGCGTCTGCAAGGTGCTTCGGGAG
  CGCATGGGCGTGCACTGCTTCCTGGGCCTCACAGCCACAGCCACACGCCGCACTGCCAGTGACGTGGCAC
  AGCACCTGGCTGTGGCTGAAGAGCCTGACCTCCACGGGCCAGCCCCAGTTCCCACCAACCTGCACCTTTC
  CGTGTCCATGGACAGGGACACAGACCAGGCACTGTTGACGCTGCTGCAAGGCAAACGTTTTCAAAACCTC
  GATTCCATTATCATTTACTGCAACCGGCGCGAGGACACAGAGCGGATCGCTGCGCTCCTCCGAACCTGCC
  TGCACGCAGCCTGGGTCCCAGGGTCTGGAGGTCGTGCCCCCAAAACCACAGCCGAGGCCTACCACGCGGG
  CATGTGCAGCCGGGAACGGCGGCGGGTACAGCGAGCCTTCATGCAGGGCCAGTTGCGGGTGGTGGTGGCC
  ACGGTGGCCTTTGGGATGGGGCTGGACCGGCCAGATGTGCGGGCTGTGCTGCATCTGGGGCTGCCCCCAA
  GCTTCGAGAGCTACGTGCAGGCCGTGGGCCGGGCCGGGCGTGACGGGCAGCCTGCCCACTGCCACCTCTT
  CCTGCAGCCCCAGGGCGAAGACCTGCGAGAGCTGCGCAGACATGTGCACGCCGACAGCACGGACTTCCTG
  GCTGTGAAGAGGCTGGTACAGCGCGTGTTCCCAGCCTGCACCTGCACCTGCACCAGGCCGCCCTCGGAGC
  AGGAAGGGGCCGTGGGTGGGGAGAGGCCTGTGCCCAAGTACCCCCCTCAAGAGGCTGAGCAGCTTAGCCA
  CCAAGCAGCCCCAGGACCCAGAAGGGTCTGCATGGGCCATGAGCGGGCACTCCCAATACAGCTTACCGTA
  CAGGCTTTGGACATGCCGGAGGAGGCCATCGAGACTTTGCTGTGCTACCTGGAGCTGCACCCACACCACT
  GGCTGGAGCTGCTGGCGACCACCTATACCCATTGCCGTCTGAACTGCCCTGGGGGCCCTGCCCAGCTCCA
  GGCCCTGGCCCACAGGTGTCCCCCTTTGGCTGTGTGCTTGGCCCAGCAGCTGCCTGAGGACCCAGGGCAA
  GGCAGCAGCTCCGTGGAGTTTGACATGGTCAAGCTGGTGGACTCCATGGGCTGGGAGCTGGCCTCTGTGC
  GGCGGGCTCTCTGCCAGCTGCAGTGGGACCACGAGCCCAGGACAGGTGTGCGGCGTGGGACAGGGGTGCT
  TGTGGAGTTCAGTGAGCTGGCCTTCCACCTTCGCAGCCCGGGGGACTTGACCGCTGAGGAGAAGGACCAG
  ATATGTGACTTCCTCTATGGCCGTGTGCAGGCCCGGGAGCGCCAGGCCCTGGCCCGTCTGCGCAGAACCT
  TCCAGGCCTTTCACAGCGTAGCCTTCCCCAGCTGCGGGCCCTGCCTGGAGCAGCAGGATGAGGAGCGCAG
  CACCAGGCTCAAGGACCTGCTCGGCCGCTACTTTGAGGAAGAGGAAGGGCAGGAGCCGGGAGGCATGGAG
  GACGCACAGGGCCCCGAGCCAGGGCAGGCCAGACTCCAGGATTGGGAGGACCAGGTCCGCTGCGACATCC
  GCCAGTTCCTGTCCCTGAGGCCAGAGGAGAAGTTCTCCAGCAGGGCTGTGGCCCGCATCTTCCACGGCAT
  CGGAAGCCCCTGCTACCCGGCCCAGGTGTACGGGCAGGACCGACGCTTCTGGAGAAAATACCTGCACCTG
  AGCTTCCATGCCCTGGTGGGCCTGGCCACGGAAGAGCTCCTGCAGGTGGCCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215399 representing NM_004260
  Red=Cloning site Green=Tags(s)
  
  MERLRDVRERLQAWERAFRRQRGRRPSQDDVEAAPEETRALYREYRTLKRTTGQAGGGLRSSESLPAAAE
  EAPEPRCWGPHLNRAATKSPQSTPGRSRQGSVPDYGQRLKANLKGTLQAGPALGRRPWPLGRASSKASTP
  KPPGTGPVPSFAEKVSDEPPQLPEPQPRPGRLQHLQASLSQRLGSLDPGWLQRCHSEVPDFLGAPKACRP
  DLGSEESQLLIPGESAVLGPGAGSQGPEASAFQEVSIRVGSPQPSSSGGEKRRWNEEPWESPAQVQQESS
  QAGPPSEGAGAVAVEEDPPGEPVQAQPPQPCSSPSNPRYHGLSPSSQARAGKAEGTAPLHIFPRLARHDR
  GNYVRLNMKQKHYVRGRALRSRLLRKQAWKQKWRKKGECFGGGGATVTTKESCFLNEQFDHWAAQCPRPA
  SEEDTDAVGPEPLVPSPQPVPEVPSLDPTVLPLYSLGPSGQLAETPAEVFQALEQLGHQAFRPGQERAVM
  RILSGISTLLVLPTGAGKSLCYQLPALLYSRRSPCLTLVVSPLLSLMDDQVSGLPPCLKAACIHSGMTRK
  QRESVLQKIRAAQVHVLMLTPEALVGAGGLPPAAQLPPVAFACIDEAHCLSQWSHNFRPCYLRVCKVLRE
  RMGVHCFLGLTATATRRTASDVAQHLAVAEEPDLHGPAPVPTNLHLSVSMDRDTDQALLTLLQGKRFQNL
  DSIIIYCNRREDTERIAALLRTCLHAAWVPGSGGRAPKTTAEAYHAGMCSRERRRVQRAFMQGQLRVVVA
  TVAFGMGLDRPDVRAVLHLGLPPSFESYVQAVGRAGRDGQPAHCHLFLQPQGEDLRELRRHVHADSTDFL
  AVKRLVQRVFPACTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTV
  QALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQ
  GSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQ
  ICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGME
  DAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHL
  SFHALVGLATEELLQVAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004260
• ORF Size: 3624 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004260.4
• RefSeq Size: 3820 bp
• RefSeq ORF: 3627 bp
• Locus ID: 9401
• UniProt ID: O94761
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 132.9 kDa
• Gene Summary: The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010]