TMEM16K (ANO10) (NM_018075) Human Tagged ORF Clone

CAT#: RC215283

ANO10 (Myc-DDK-tagged)-Human anoctamin 10 (ANO10), transcript variant 1

Product Images

Circular map for RC215283

• 

  Western blot validation of overexpression lysate (Cat# LY413334) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215283 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM16K
• Synonyms: SCAR10; TMEM16K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215283 representing NM_018075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGACCTTATCAGCTTTGGATACTTCTGAGAGTTCTTTCACACCTTTGGTGGTCATAGAACTTG
  CTCAGGATGTCAAAGAAGAAACCAAAGAATGGCTGAAAAACAGAATTATAGCTAAAAAAAAAGATGGAGG
  TGCCCAGTTGTTGTTTAGACCATTGTTAAATAAATATGAACAAGAAACACTAGAAAATCAGAACTTATAT
  CTTGTTGGTGCCTCCAAGATTAGAATGTTACTAGGGGCAGAAGCAGTGGGATTGGTAAAAGAGTGCAATG
  ATAACACCATGAGAGCCTTCACATACAGAACCAGACAGAACTTCAAAGGTTTTGATGATAACAATGATGA
  TTTCCTGACAATGGCAGAATGTCAATTCATTATCAAACATGAACTTGAAAATCTTAGAGCTAAAGATGAA
  AAAATGATCCCTGGTTACCCTCAGGCAAAGTTGTATCCAGGAAAATCATTGTTGAGAAGATTGCTCACGT
  CTGGCATCGTGATTCAGGTGTTTCCACTGCATGACAGTGAAGCCCTGAAGAAGCTTGAGGACACCTGGTA
  CACTCGGTTTGCTTTGAAGTATCAGCCCATAGACAGTATTCGTGGCTACTTTGGGGAAACAATTGCTCTG
  TACTTTGGATTTTTGGAGTATTTCACTTTTGCATTAATCCCCATGGCTGTCATTGGGTTACCTTACTACT
  TGTTTGTGTGGGAAGACTATGACAAGTACGTGATCTTTGCCTCGTTCAACCTCATCTGGTCCACGGTGAT
  TCTGGAACTGTGGAAGCGTGGCTGTGCCAACATGACCTACAGGTGGGGGACACTGCTCATGAAGAGAAAG
  TTTGAGGAGCCCCGGCCAGGATTTCATGGTGTCTTGGGTATCAATTCCATCACTGGGAAGGAGGAGCCTC
  TGTACCCCAGCTACAAGAGACAGTTGCGCATTTACCTGGTCTCCCTGCCATTCGTGTGCCTCTGCCTCTA
  TTTCTCACTGTATGTCATGATGATTTACTTCGACATGGAGGTTTGGGCCTTGGGTCTACATGAGAACAGC
  GGGTCTGAGTGGACCAGTGTCCTGTTGTATGTGCCCAGCATCATCTATGCCATTGTGATTGAGATCATGA
  ATCGTCTCTATCGATATGCTGCCGAGTTTTTAACTTCATGGGAGAATCACAGATTGGAATCTGCCTATCA
  GAACCATCTAATTCTGAAAGTTTTAGTGTTCAACTTCCTCAATTGCTTTGCCTCACTCTTCTATATTGCC
  TTTGTCTTGAAAGATATGAAGCTTTTGCGCCAGAGCTTGGCCACTCTCCTAATTACCTCCCAGATCCTCA
  ACCAAATTATGGAATCTTTTCTTCCTTATTGGCTCCAAAGGAAGCATGGTGTGCGGGTGAAGAGGAAGGT
  GCAGGCTTTAAAGGCAGACATTGATGCTACATTATATGAACAAGTCATCCTGGAAAAAGAAATGGGAACT
  TATTTGGGCACCTTTGATGATTACTTGGAGTTATTCCTGCAGTTTGGTTATGTGAGCCTTTTCTCCTGTG
  TTTACCCATTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAAGTAAATTCAGATGCCTTAAAAAT
  GTGCAGGGTCTTCAAACGTCCATTCTCAGAACCTTCAGCCAATATTGGTGTGTGGCAGTTGGCTTTTGAA
  ACGATGAGTGTTATATCTGTGGTCACTAACTGTGCGCTGATTGGAATGTCACCACAAGTGAATGCAGTCT
  TTCCAGAATCAAAAGCAGACCTCATTTTGATTGTAGTAGCAGTGGAGCACGCACTCCTGGCTTTAAAGTT
  TATACTTGCATTTGCCATACCTGATAAGCCACGGCATATCCAGATGAAACTAGCCAGACTGGAATTTGAG
  TCTTTGGAGGCACTCAAGCAGCAGCAAATGAAGCTCGTGACCGAGAACCTGAAGGAGGAACCAATGGAAA
  GCGGGAAGGAGAAGGCAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215283 representing NM_018075
  Red=Cloning site Green=Tags(s)
  
  MKVTLSALDTSESSFTPLVVIELAQDVKEETKEWLKNRIIAKKKDGGAQLLFRPLLNKYEQETLENQNLY
  LVGASKIRMLLGAEAVGLVKECNDNTMRAFTYRTRQNFKGFDDNNDDFLTMAECQFIIKHELENLRAKDE
  KMIPGYPQAKLYPGKSLLRRLLTSGIVIQVFPLHDSEALKKLEDTWYTRFALKYQPIDSIRGYFGETIAL
  YFGFLEYFTFALIPMAVIGLPYYLFVWEDYDKYVIFASFNLIWSTVILELWKRGCANMTYRWGTLLMKRK
  FEEPRPGFHGVLGINSITGKEEPLYPSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEVWALGLHENS
  GSEWTSVLLYVPSIIYAIVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLILKVLVFNFLNCFASLFYIA
  FVLKDMKLLRQSLATLLITSQILNQIMESFLPYWLQRKHGVRVKRKVQALKADIDATLYEQVILEKEMGT
  YLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFSEPSANIGVWQLAFE
  TMSVISVVTNCALIGMSPQVNAVFPESKADLILIVVAVEHALLALKFILAFAIPDKPRHIQMKLARLEFE
  SLEALKQQQMKLVTENLKEEPMESGKEKAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018075
• ORF Size: 1980 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018075.5
• RefSeq Size: 2743 bp
• RefSeq ORF: 1983 bp
• Locus ID: 55129
• UniProt ID: Q9NW15
• Cytogenetics: 3p22.1-p21.33
• Domains: DUF590
• Protein Families: Transmembrane
• MW: 76.1 kDa
• Gene Summary: The transmembrane protein encoded by this gene belongs to the anoctamin family of calcium-activated chloride channels, also known as the transmembrane 16 family. The encoded protein contains eight transmembrane domains with cytosolic N- and C-termini. Defects in this gene may cause autosomal recessive spinocerebellar ataxia-10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]