Synaptojanin (SYNJ1) (NM_203446) Human Tagged ORF Clone

CAT#: RC215278

SYNJ1 (Myc-DDK-tagged)-Human synaptojanin 1 (SYNJ1), transcript variant 2

Product Images

Circular map for RC215278

• 

  Western blot validation of overexpression lysate (Cat# LY431693) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228665 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SYNJ1 protein (Cat# TP315278). The protein was produced from HEK293T cells transfected with SYNJ1 cDNA clone (Cat# RC215278) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Synaptojanin
• Synonyms: DEE53; EIEE53; INPP5G; PARK20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215278 representing NM_203446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCAGTAAAGGATTCCGGATCTATCACAAATTGGATCCCCCACCTTTCAGCCTCATAGTGGAAA
  CTAGGCATAAGGAAGAATGTCTCATGTTCGAGTCTGGGGCTGTCGCTGTGCTCTCATCTGCAGAAAAAGA
  GGCAATCAAGGGTACATACTCCAAAGTACTGGATGCATATGGACTCTTAGGTGTTCTGCGGTTAAATCTT
  GGTGATACTATGTTACATTATCTGGTCCTAGTCACTGGATGTATGTCTGTTGGAAAAATTCAAGAATCTG
  AAGTTTTCCGAGTTACTTCCACTGAGTTTATATCACTGCGAATCGATTCTTCAGATGAGGATCGCATTTC
  AGAAGTGCGGAAAGTTTTGAATTCAGGAAACTTTTATTTTGCATGGTCTGCATCTGGCATCAGTTTAGAT
  TTGAGTCTTAATGCGCATCGTAGCATGCAAGAACAGACAACTGATAATAGATTTTTCTGGAATCAGTCTT
  TGCATTTGCATCTCAAACACTATGGCGTGAATTGTGATGACTGGTTATTACGTCTTATGTGTGGAGGAGT
  AGAAATCAGAACAATTTATGCTGCTCATAAACAGGCGAAGGCTTGCCTCATTTCAAGATTAAGCTGTGAA
  CGAGCTGGGACCAGGTTTAATGTCCGGGGAACAAATGATGATGGTCATGTTGCCAATTTTGTAGAAACAG
  AACAGGTTGTGTACTTAGATGACTCAGTTTCTTCCTTCATACAAATCCGAGGATCTGTTCCATTGTTCTG
  GGAGCAACCAGGGTTGCAAGTGGGATCTCATCGTGTCCGTATGTCAAGGGGATTTGAAGCCAATGCACCT
  GCTTTTGACAGGCATTTTAGAACACTTAAGAACTTATATGGTAAACAAATAATAGTAAATTTGCTTGGAT
  CTAAGGAAGGTGAACATATGCTAAGTAAAGCTTTCCAGAGTCATTTGAAAGCTTCTGAACATGCTGCTGA
  TATCCAGATGGTGAATTTTGACTATCATCAAATGGTTAAGGGAGGAAAGGCAGAAAAATTACATAGTGTT
  CTTAAACCTCAAGTCCAGAAGTTTCTAGATTATGGATTTTTTTATTTCAATGGAAGTGAAGTTCAAAGAT
  GCCAGAGTGGTACAGTTCGAACAAACTGCTTGGATTGTCTTGATAGAACAAATAGTGTGCAGGCATTTCT
  TGGCTTAGAGATGCTAGCTAAACAGTTGGAAGCTCTTGGTTTAGCTGAAAAGCCTCAGTTGGTGACTCGC
  TTTCAAGAAGTTTTTCGGTCAATGTGGTCCGTGAATGGTGATTCAATCAGTAAGATATATGCAGGAACTG
  GAGCTCTTGAAGGGAAAGCGAAGTTAAAAGATGGTGCTCGCTCTGTTACCCGAACAATTCAGAATAACTT
  CTTTGACAGCTCCAAGCAAGAGGCCATTGATGTTTTGCTACTGGGAAATACTCTGAATAGTGATTTAGCT
  GACAAAGCTCGAGCACTTTTAACTACTGGAAGTTTGCGTGTTTCTGAGCAGACATTACAGTCAGCATCTT
  CTAAAGTACTAAAGAGCATGTGTGAGAATTTCTACAAATATTCAAAGCCTAAGAAAATTCGAGTATGTGT
  CGGAACCTGGAATGTGAATGGTGGGAAGCAATTTCGCAGCATAGCTTTTAAGAATCAGACACTCACTGAC
  TGGCTTCTTGATGCACCCAAGTTAGCTGGCATCCAGGAGTTTCAAGATAAAAGAAGTAAGCCAACTGATA
  TATTTGCAATTGGTTTTGAAGAAATGGTAGAATTGAATGCTGGAAACATTGTGAGTGCAAGCACAACAAA
  TCAGAAGCTCTGGGCTGTAGAACTTCAGAAGACAATCTCCAGAGACAACAAGTATGTGCTGCTGGCTTCT
  GAACAGTTGGTGGGCGTCTGTTTGTTTGTTTTTATCAGACCACAGCATGCTCCTTTTATCAGGGATGTTG
  CAGTTGATACTGTGAAGACTGGAATGGGAGGTGCAACTGGAAATAAGGGAGCAGTTGCAATCCGAATGCT
  CTTCCATACAACCAGCCTTTGCTTCGTCTGTAGCCACTTTGCTGCAGGGCAGTCACAAGTCAAAGAAAGA
  AATGAAGATTTTATAGAAATAGCACGAAAATTGAGTTTTCCTATGGGAAGGATGCTATTTTCCCATGACT
  ATGTATTTTGGTGTGGTGATTTCAACTATCGAATCGATCTCCCTAACGAAGAAGTTAAAGAGCTCATAAG
  ACAGCAAAATTGGGATTCTCTTATAGCAGGAGATCAACTTATCAATCAGAAAAATGCTGGACAGGTTTTT
  AGAGGATTTTTAGAAGGAAAGGTAACCTTTGCTCCGACATATAAGTATGACTTGTTTTCTGACGACTATG
  ACACCAGTGAAAAGTGCCGCACCCCTGCCTGGACAGACCGTGTCCTTTGGAGAAGGAGGAAATGGCCTTT
  TGATAGATCAGCTGAAGATCTAGATCTTCTAAATGCTAGTTTTCAAGATGAAAGCAAAATTCTGTACACG
  TGGACTCCAGGCACTTTGCTGCACTATGGAAGAGCTGAGCTGAAGACTTCTGACCACAGGCCTGTCGTTG
  CCCTGATTGATATAGATATATTTGAAGTTGAAGCTGAAGAGAGGCAAAACATTTATAAAGAAGTAATTGC
  AGTTCAGGGTCCACCAGATGGTACAGTATTGGTCTCAATCAAAAGTTCTTTACCAGAAAATAATTTTTTT
  GATGATGCCTTGATTGATGAGCTTCTGCAGCAGTTTGCAAGTTTTGGTGAAGTTATACTTATAAGATTTG
  TAGAAGATAAAATGTGGGTTACATTTTTGGAGGGAAGCTCTGCCTTGAATGTTCTGAGCCTAAATGGTAA
  AGAGTTATTGAATCGGACTATAACTATTGCTTTAAAAAGTCCAGACTGGATCAAAAATTTGGAAGAAGAA
  ATGAGTTTAGAGAAAATTAGCATTGCATTGCCATCATCAACAAGCTCTACCCTGCTTGGTGAAGATGCAG
  AGGTTGCAGCAGATTTTGATATGGAAGGTGATGTTGATGACTATAGTGCTGAAGTGGAGGAACTTCTTCC
  TCAGCATCTCCAGCCATCTTCAAGTTCCGGCCTTGGTACTTCCCCCAGCTCTTCACCCCGAACTAGTCCC
  TGCCAGTCACCTACAATATCAGAGGGTCCTGTACCTTCCCTTCCCATCAGACCAAGCCGAGCACCGTCAA
  GAACTCCTGGGCCTCCCAGTGCACAGAGTTCTCCTATTGACGCGCAGCCAGCAACGCCGCTGCCGCAGAA
  AGACCCCGCCCAGCCCTTGGAGCCCAAGCGGCCGCCGCCGCCCCGCCCGGTCGCCCCTCCCACACGCCCG
  GCTCCCCCACAGAGACCTCCTCCGCCTTCAGGGGCTAGGAGTCCTGCACCCACTAGAAAGGAATTTGGAG
  CACCCAAAAGCCCTGGAACAACAAGGAAAGATAATATAGGACGCAGTCAGCCTTCACCTCAAGCAGGACT
  TGCAGGCCCAGGACCTGCTGGATACAGTACAGCCAGACCGACGATTCCTCCTCGTGCTGGAGTTATCAGT
  GCCCCACAGAGCCACGCGCGGGCATCTGCTGGAAGACTGACTCCTGAAAGCCAAAGCAAAACATCAGAAA
  CGTCGAAAGGTTCAACTTTCCTTCCTGAACCACTGAAGCCTCAGGCTGCTTTTCCTCCGCAGTCTTCTTT
  GCCCCCGCCTGCTCAAAGGTTGCAAGAGCCTCTTGTCCCTGTGGCAGCACCTATGCCTCAGTCTGGCCCC
  CAGCCAAATTTGGAAACCCCACCACAACCACCACCTCGAAGCAGGTCATCCCATAGCTTGCCTTCAGAAG
  CTTCCTCACAACCGCAACAGGAGCAACCATCAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215278 representing NM_203446
  Red=Cloning site Green=Tags(s)
  
  MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYSKVLDAYGLLGVLRLNL
  GDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRIDSSDEDRISEVRKVLNSGNFYFAWSASGISLD
  LSLNAHRSMQEQTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCE
  RAGTRFNVRGTNDDGHVANFVETEQVVYLDDSVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAP
  AFDRHFRTLKNLYGKQIIVNLLGSKEGEHMLSKAFQSHLKASEHAADIQMVNFDYHQMVKGGKAEKLHSV
  LKPQVQKFLDYGFFYFNGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTR
  FQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLA
  DKARALLTTGSLRVSEQTLQSASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTD
  WLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVSASTTNQKLWAVELQKTISRDNKYVLLAS
  EQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKER
  NEDFIEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQVF
  RGFLEGKVTFAPTYKYDLFSDDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYT
  WTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFF
  DDALIDELLQQFASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEE
  MSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSP
  CQSPTISEGPVPSLPIRPSRAPSRTPGPPSAQSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRP
  APPQRPPPPSGARSPAPTRKEFGAPKSPGTTRKDNIGRSQPSPQAGLAGPGPAGYSTARPTIPPRAGVIS
  APQSHARASAGRLTPESQSKTSETSKGSTFLPEPLKPQAAFPPQSSLPPPAQRLQEPLVPVAAPMPQSGP
  QPNLETPPQPPPRSRSSHSLPSEASSQPQQEQPSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203446
• ORF Size: 3885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203446.1, NP_982271.1
• RefSeq Size: 7039 bp
• RefSeq ORF: 3936 bp
• Locus ID: 8867
• UniProt ID: O43426
• Cytogenetics: 21q22.11
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 149.1 kDa
• Gene Summary: This gene encodes a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]