IMPDH1 (NM_000883) Human Tagged ORF Clone

CAT#: RC215206

IMPDH1 (Myc-DDK-tagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 1

Product Images

Circular map for RC215206

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  Western blot validation of overexpression lysate (Cat# LY400315) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215206 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IMPDH1 protein (Cat# TP315206). The protein was produced from HEK293T cells transfected with IMPDH1 cDNA clone (Cat# RC215206) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IMPDH1
• Synonyms: IMPD; IMPD1; IMPDH-I; LCA11; RP10; sWSS2608
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215206 representing NM_000883
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGCCACTCACTCCACCACCGCTGCAGGGAGGCGGAGCCGCCGCTGTTCCGGAGCCCGGAGCCC
  GGCAACACCCGGGACACGAGACGGCGGCGCAGCGGTACAGCGCCCGACTGCTGCAGGCCGGCTACGAGCC
  CGAGAGCCCTAGATTGGACCTCGCTACACACCCGACGACACCCCGTTCAGAACTATCTTCAGTGGTCTTA
  CTGGCAGGTGTTGGTGTCCAGATGGATCGCCTTCGCAGGGCTAGCATGGCGGACTACCTGATCAGCGGCG
  GCACCGGCTACGTGCCCGAGGATGGGCTCACCGCGCAGCAGCTCTTCGCCAGCGCCGACGGCCTCACCTA
  CAACGACTTCCTGATTCTCCCAGGATTCATAGACTTCATAGCTGATGAGGTGGACCTGACCTCAGCCCTG
  ACCCGGAAGATCACGCTGAAGACGCCACTGATCTCCTCCCCCATGGACACTGTGACAGAGGCTGACATGG
  CCATTGCCATGGCTCTGATGGGAGGTATTGGTTTCATTCACCACAACTGCACCCCAGAGTTCCAGGCCAA
  CGAGGTGCGGAAGGTCAAGAAGTTTGAACAGGGCTTCATCACGGACCCTGTGGTGCTGAGCCCCTCGCAC
  ACTGTGGGCGATGTGCTGGAGGCCAAGATGCGGCATGGCTTCTCTGGCATCCCCATCACTGAGACGGGCA
  CCATGGGCAGCAAGCTGGTGGGCATCGTCACCTCCCGAGACATCGACTTTCTTGCTGAGAAGGACCACAC
  CACCCTCCTCAGTGAGGTGATGACGCCAAGGATTGAACTGGTGGTGGCTCCAGCAGGTGTGACGTTGAAA
  GAGGCAAATGAGATCCTGCAGCGTAGCAAGAAAGGGAAGCTGCCTATCGTCAATGATTGCGATGAGCTGG
  TGGCCATCATCGCCCGCACCGACCTGAAGAAGAACCGAGACTACCCTCTGGCCTCCAAGGATTCCCAGAA
  GCAGCTGCTCTGTGGGGCAGCTGTGGGCACCCGTGAGGATGACAAATACCGTCTGGACCTGCTCACCCAG
  GCGGGCGTCGACGTCATAGTCTTGGACTCGTCCCAAGGGAATTCGGTGTATCAGATCGCCATGGTGCATT
  ACATCAAACAGAAGTACCCCCACCTCCAGGTGATTGGGGGGAACGTGGTGACAGCAGCCCAGGCCAAGAA
  CCTGATTGATGCTGGTGTGGACGGGCTGCGCGTGGGCATGGGCTGCGGCTCCATCTGCATCACCCAGGAA
  GTGATGGCCTGTGGTCGGCCCCAGGGCACTGCTGTGTACAAGGTGGCTGAGTATGCCCGGCGCTTTGGTG
  TGCCCATCATAGCCGATGGCGGCATCCAGACCGTGGGACACGTGGTCAAGGCCCTGGCCCTTGGAGCCTC
  CACAGTGATGATGGGCTCCCTGCTGGCCGCCACTACGGAGGCCCCTGGCGAGTACTTCTTCTCAGACGGG
  GTGCGGCTCAAGAAGTACCGGGGCATGGGCTCACTGGATGCCATGGAGAAGAGCAGCAGCAGCCAGAAAC
  GATACTTCAGCGAGGGGGATAAAGTGAAGATCGCACAGGGTGTCTCGGGCTCCATCCAGGACAAAGGATC
  CATTCAGAAGTTCGTGCCCTACCTCATAGCAGGCATCCAACACGGCTGCCAGGATATCGGGGCCCGCAGC
  CTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCTCAAGTTTGAGAAGCGGACCATGTCGGCCCAGA
  TTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAAAGCGGCTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215206 representing NM_000883
  Red=Cloning site Green=Tags(s)
  
  MEGPLTPPPLQGGGAAAVPEPGARQHPGHETAAQRYSARLLQAGYEPESPRLDLATHPTTPRSELSSVVL
  LAGVGVQMDRLRRASMADYLISGGTGYVPEDGLTAQQLFASADGLTYNDFLILPGFIDFIADEVDLTSAL
  TRKITLKTPLISSPMDTVTEADMAIAMALMGGIGFIHHNCTPEFQANEVRKVKKFEQGFITDPVVLSPSH
  TVGDVLEAKMRHGFSGIPITETGTMGSKLVGIVTSRDIDFLAEKDHTTLLSEVMTPRIELVVAPAGVTLK
  EANEILQRSKKGKLPIVNDCDELVAIIARTDLKKNRDYPLASKDSQKQLLCGAAVGTREDDKYRLDLLTQ
  AGVDVIVLDSSQGNSVYQIAMVHYIKQKYPHLQVIGGNVVTAAQAKNLIDAGVDGLRVGMGCGSICITQE
  VMACGRPQGTAVYKVAEYARRFGVPIIADGGIQTVGHVVKALALGASTVMMGSLLAATTEAPGEYFFSDG
  VRLKKYRGMGSLDAMEKSSSSQKRYFSEGDKVKIAQGVSGSIQDKGSIQKFVPYLIAGIQHGCQDIGARS
  LSVLRSMMYSGELKFEKRTMSAQIEGGVHGLHSYEKRLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000883
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000883.4
• RefSeq Size: 2880 bp
• RefSeq ORF: 1800 bp
• Locus ID: 3614
• UniProt ID: P20839
• Cytogenetics: 7q32.1
• Domains: CBS, IMPDH
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• MW: 64.1 kDa
• Gene Summary: The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]