SPEF1 (NM_015417) Human Tagged ORF Clone

CAT#: RC215184

SPEF1 (Myc-DDK-tagged)-Human sperm flagellar 1 (SPEF1)

Product Images

Circular map for RC215184

• 

  Western blot validation of overexpression lysate (Cat# LY414579) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215184 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPEF1
• Synonyms: C20orf28; CLAMP; SPEF1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215184 representing NM_015417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCAGCGTGGACGAGGAGGCGCTGCACCAGCTGTACCTGTGGGTAGACAACATCCCTCTGTCCC
  GGCCCAAGCGAAACCTCTCCCGGGACTTTAGCGATGGAGTCCTTGTTGCAGAGGTCATCAAGTTTTACTT
  CCCCAAGATGGTGGAGATGCACAATTATGTCCCCGCCAACTCTCTCCAGCAGAAGCTCAGCAACTGGGGT
  CATCTGAACAGGAAGGTACTGAAGAGGCTGAACTTTTCAGTACCGGATGACGTGATGCGCAAGATCGCGC
  AGTGCGCCCCAGGCGTGGTGGAGCTGGTGCTCATCCCGCTGAGGCAGCGCCTGGAGGAGAGGCAGAGGCG
  CAGGAAGCAGGGCGCCGGCTCCTTACAGGAGCTGGCTCCCCAGGATGGCAGTGGCTACATGGATGTGGGT
  GTATCCCAGAAGGCCCGAGGTGAAGGTGTCCCGGACCCCCAGGGAGGGGGTCAGCTCAGCTGGGACCGGC
  CGCCGGCGCCTCGGCCTCCAGCGTATAACCGGGCGTTGCAGGGCGACCCCAGCTTCGTCCTCCAGATCGC
  TGAAAAGGAGCAGGAGCTGTTGGCCTCTCAAGAGACCGTGCAGGTCCTGCAGATGAAGGTAAGGCGCCTG
  GAGCACCTGCTCCAGCTCAAGAATGTGCGGATCGAAGACCTCTCCCGGCGGCTCCAGCAGGCGGAGCGTA
  AGCAGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215184 representing NM_015417
  Red=Cloning site Green=Tags(s)
  
  MASSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAEVIKFYFPKMVEMHNYVPANSLQQKLSNWG
  HLNRKVLKRLNFSVPDDVMRKIAQCAPGVVELVLIPLRQRLEERQRRRKQGAGSLQELAPQDGSGYMDVG
  VSQKARGEGVPDPQGGGQLSWDRPPAPRPPAYNRALQGDPSFVLQIAEKEQELLASQETVQVLQMKVRRL
  EHLLQLKNVRIEDLSRRLQQAERKQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015417
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015417.5
• RefSeq Size: 1597 bp
• RefSeq ORF: 711 bp
• Locus ID: 25876
• UniProt ID: Q9Y4P9
• Cytogenetics: 20p13
• MW: 26.8 kDa
• Gene Summary: Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (By similarity).[UniProtKB/Swiss-Prot Function]