PSD95 (DLG4) (NM_001365) Human Tagged ORF Clone

CAT#: RC215178

DLG4 (Myc-DDK-tagged)-Human discs, large homolog 4 (Drosophila) (DLG4), transcript variant 1

Product Images

Circular map for RC215178

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DLG4 (Cat# RC215178, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLG4 (1:2000) (Cat# TA809015). Positive lysates LY419977 (100ug) and LC419977 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY419977) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215178 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DLG4 protein (Cat# TP315178). The protein was produced from HEK293T cells transfected with DLG4 cDNA clone (Cat# RC215178) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSD95
• Synonyms: MRD62; PSD95; SAP-90; SAP90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215178 representing NM_001365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGAGACCAAGAGCTCCCAGGTCAGCCCTCTGGCTCCTGGCACCCCCACTGCTGCGGTGGGCAC
  CCCCACTCCTCACAGTGCTGCATAGCGACCTCTTCCAGGCCTTGCTGGACATCCTGGACTATTATGAGGC
  TTCCCTCTCAGAGAGTCAGAAATACCGCTACCAAGATGAAGACACGCCCCCTCTGGAGCACAGCCCGGCC
  CACCTCCCCAACCAGGCCAATTCTCCCCCAGTGATTGTCAACACAGATACCCTAGAAGCCCCAGGATATG
  AGTTGCAGGTGAACGGGACCGAGGGGGAGATGGAATACGAGGAAATCACATTGGAAAGGGGTAACTCAGG
  TCTGGGCTTCAGCATCGCAGGTGGCACTGACAACCCACACATCGGTGACGACCCATCCATTTTCATCACC
  AAGATCATTCCTGGTGGGGCTGCGGCCCAGGATGGCCGCCTCAGGGTCAACGACAGCATCCTGTTTGTAA
  ATGAAGTGGACGTGCGCGAGGTGACCCACTCAGCGGCGGTGGAAGCCCTCAAAGAGGCAGGCTCCATCGT
  TCGCCTCTATGTCATGCGCCGGAAGCCCCCGGCTGAGAAGGTCATGGAGATCAAGCTCATCAAGGGGCCT
  AAAGGTCTTGGCTTCAGCATCGCAGGGGGCGTAGGGAACCAGCACATCCCAGGAGATAATAGCATCTATG
  TAACAAAGATCATCGAAGGGGGTGCTGCCCACAAGGATGGGAGGTTGCAGATTGGAGACAAGATCCTGGC
  GGTCAACAGTGTGGGGCTAGAGGACGTCATGCATGAAGATGCTGTGGCAGCCCTGAAGAACACGTATGAT
  GTTGTCTACCTAAAGGTGGCCAAGCCCAGCAATGCCTACCTGAGTGACAGCTATGCTCCCCCAGACATCA
  CAACCTCTTATTCCCAGCACCTGGACAATGAGATCAGTCACAGCAGCTACCTGGGCACCGACTACCCCAC
  AGCCATGACCCCCACTTCCCCTCGGCGCTACTCTCCAGTGGCCAAGGACCTGCTCGGGGAGGAAGACATT
  CCCCGAGAACCGAGGCGAATTGTGATCCACCGGGGCTCCACGGGCCTGGGCTTCAACATCGTGGGTGGCG
  AGGACGGTGAAGGCATCTTCATCTCCTTTATCCTGGCCGGGGGCCCTGCAGACCTCAGTGGGGAGCTGCG
  GAAGGGGGACCAGATCCTGTCGGTCAACGGTGTGGACCTCCGAAATGCCAGCCATGAGCAGGCTGCCATT
  GCCCTGAAGAATGCGGGTCAGACGGTCACGATCATTGCTCAGTATAAACCAGAAGAGTACAGCCGATTCG
  AGGCCAAGATCCACGACCTTCGGGAACAGCTCATGAACAGCAGCCTGGGCTCAGGGACTGCGTCCCTGCG
  GAGCAACCCCAAAAGGGGTTTCTACATCAGGGCCCTGTTTGATTACGACAAGACCAAGGACTGCGGCTTC
  CTGAGCCAGGCCCTGAGCTTCCGCTTTGGGGATGTGCTGCATGTCATCGATGCTAGTGATGAGGAGTGGT
  GGCAGGCACGGCGGGTCCACTCTGACAGTGAGACCGACGACATTGGGTTCATCCCCAGCAAACGGCGGGT
  TGAGCGACGAGAGTGGTCAAGGTTAAAGGCCAAGGACTGGGGCTCCAGCTCTGGATCGCAGGGTCGAGAA
  GACTCGGTTCTGAGCTACGAGACAGTGACGCAGATGGAAGTGCACTATGCTCGCCCCATCATCATCCTTG
  GGCCCACCAAGGACCGCGCCAACGATGATCTTCTCTCCGAGTTCCCCGACAAGTTTGGATCCTGTGTTCC
  CCATACGACACGGCCCAAGCGGGAGTATGAGATAGATGGCCGGGATTACCACTTTGTGTCGTCCCGGGAG
  AAAATGGAGAAGGACATTCAGGCGCACAAGTTCATTGAGGCCGGCCAGTACAACAGCCACCTCTATGGGA
  CCAGCGTCCAGTCCGTGCGAGAGGTGGCAGAGCAGGGGAAGCACTGCATCCTCGATGTCTCGGCCAATGC
  CGTGCGGCGGCTGCAGGCGGCCCACCTGCACCCCATCGCCATCTTCATCCGCCCCCGCTCCCTGGAGAAT
  GTGCTAGAGATTAACAAGCGGATCACAGAGGAGCAAGCCCGCAAAGCCTTCGACAGAGCCACCAAGCTGG
  AGCAGGAGTTCACAGAGTGCTTCTCAGCCATCGTGGAGGGTGACAGCTTTGAGGAGATCTACCACAAGGT
  GAAGCGTGTCATCGAGGACCTCTCAGGCCCCTACATCTGGGTTCCAGCCCGAGAGAGACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215178 representing NM_001365
  Red=Cloning site Green=Tags(s)
  
  MSQRPRAPRSALWLLAPPLLRWAPPLLTVLHSDLFQALLDILDYYEASLSESQKYRYQDEDTPPLEHSPA
  HLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFIT
  KIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGP
  KGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYD
  VVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDI
  PREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAI
  ALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGF
  LSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGRE
  DSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSRE
  KMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLEN
  VLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001365
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001365.4
• RefSeq Size: 3995 bp
• RefSeq ORF: 2304 bp
• Locus ID: 1742
• UniProt ID: P78352
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• MW: 85.2 kDa
• Gene Summary: This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]