Atrophin 1 (ATN1) (NM_001007026) Human Tagged ORF Clone

CAT#: RC215062

ATN1 (Myc-DDK-tagged)-Human atrophin 1 (ATN1), transcript variant 1

Product Images

Circular map for RC215062

• 

  Western blot validation of overexpression lysate (Cat# LY423568) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215062 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atrophin 1
• Synonyms: B37; CHEDDA; D12S755E; DRPLA; HRS; NOD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215062 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACACGACAGAATAAAGACTCGATGTCAATGAGGAGTGGACGGAAGAAAGAGGCCCCTGGGCCCC
  GGGAAGAACTGAGATCGAGGGGCCGGGCCTCCCCTGGAGGGGTCAGCACGTCCAGCAGTGATGGCAAAGC
  TGAGAAGTCCAGGCAGACAGCCAAGAAGGCCCGAGTAGAGGAAGCCTCCACCCCAAAGGTCAACAAGCAG
  GGTCGGAGTGAGGAGATCTCAGAGAGTGAAAGTGAGGAGACCAATGCACCAAAAAAGACCAAAACTGAGC
  AGGAACTCCCTCGGCCACAGTCTCCCTCCGATCTGGATAGCTTGGACGGGCGGAGCCTTAATGATGATGG
  CAGCAGCGACCCTAGGGATATCGACCAGGACAACCGAAGCACGTCCCCCAGTATCTACAGCCCTGGAAGT
  GTGGAGAATGACTCTGACTCATCTTCTGGCCTGTCCCAGGGCCCAGCCCGCCCCTACCACCCACCTCCAC
  TCTTTCCTCCTTCCCCTCAACCGCCAGACAGCACCCCTCGACAGCCAGAGGCTAGCTTTGAACCCCATCC
  TTCTGTGACACCCACTGGATATCATGCTCCCATGGAGCCCCCCACATCTCGAATGTTCCAGGCTCCTCCT
  GGGGCCCCTCCCCCTCACCCACAGCTCTATCCTGGGGGCACTGGTGGAGTTTTGTCTGGACCCCCAATGG
  GTCCCAAGGGGGGAGGGGCTGCCTCATCAGTGGGGGGCCCTAATGGGGGTAAGCAGCACCCCCCACCCAC
  TACTCCCATTTCAGTATCAAGCTCTGGGGCTAGTGGTGCTCCCCCAACAAAGCCGCCTACCACTCCAGTG
  GGTGGTGGGAACCTACCTTCTGCTCCACCACCAGCCAACTTCCCCCATGTGACACCGAACCTGCCTCCCC
  CACCTGCCCTGAGACCCCTCAACAATGCATCAGCCTCTCCCCCTGGCCTGGGGGCCCAACCACTACCTGG
  TCATCTGCCCTCTCCCCACGCCATGGGACAGGGTATGGGTGGACTTCCTCCTGGCCCAGAGAAGGGCCCA
  ACTCTGGCTCCTTCACCCCACTCTCTGCCTCCTGCTTCCTCTTCTGCTCCAGCGCCCCCCATGAGGTTTC
  CTTATTCATCCTCTAGTAGTAGCTCTGCAGCAGCCTCCTCTTCCAGTTCTTCCTCCTCTTCCTCTGCCTC
  CCCCTTCCCAGCTTCCCAGGCATTGCCCAGCTACCCCCACTCTTTCCCTCCCCCAACAAGCCTCTCTGTC
  TCCAATCAGCCCCCCAAGTATACTCAGCCTTCTCTCCCATCCCAGGCTGTGTGGAGCCAGGGTCCCCCAC
  CACCTCCTCCCTATGGCCGCCTCTTAGCCAACAGCAATGCCCATCCAGGCCCCTTCCCTCCCTCTACTGG
  GGCCCAGTCCACCGCCCACCCACCAGTCTCAACACATCACCATCACCACCAGCAACAGCAACAGCAGCAG
  CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCACGGAAACTCTGGGCCCCCTCCTCCTG
  GAGCATTTCCCCACCCACTGGAGGGCGGTAGCTCCCACCACGCACACCCTTACGCCATGTCTCCCTCCCT
  GGGGTCTCTGAGGCCCTACCCACCAGGGCCAGCACACCTGCCCCCACCTCACAGCCAGGTGTCCTACAGC
  CAAGCAGGCCCCAATGGCCCTCCAGTCTCTTCCTCTTCCAACTCTTCCTCTTCCACTTCTCAAGGGTCCT
  ACCCATGTTCACACCCCTCCCCTTCCCAGGGCCCTCAAGGGGCGCCCTACCCTTTCCCACCGGTGCCTAC
  GGTCACCACCTCTTCGGCTACCCTTTCCACGGTCATTGCCACCGTGGCTTCCTCGCCAGCAGGCTACAAA
  ACGGCCTCCCCACCTGGGCCCCCACCGTACGGAAAGAGAGCCCCGTCCCCGGGGGCCTACAAGACAGCCA
  CCCCACCCGGATACAAACCCGGGTCGCCTCCCTCCTTCCGAACGGGGACCCCACCGGGCTATCGAGGAAC
  CTCGCCACCTGCAGGCCCAGGGACCTTCAAGCCGGGCTCGCCCACCGTGGGACCTGGGCCCCTGCCACCT
  GCGGGGCCCTCAGGCCTGCCATCGCTGCCACCACCACCTGCGGCCCCTGCCTCAGGGCCGCCCCTGAGCG
  CCACGCAGATCAAACAGGAGCCGGCTGAGGAGTATGAGACCCCCGAGAGCCCGGTGCCCCCAGCCCGCAG
  CCCCTCGCCCCCTCCCAAGGTGGTAGATGTACCCAGCCATGCCAGTCAGTCTGCCAGGTTCAACAAACAC
  CTGGATCGCGGCTTCAACTCGTGCGCGCGCAGCGACCTGTACTTCGTGCCACTGGAGGGCTCCAAGCTGG
  CCAAGAAGCGGGCCGACCTGGTGGAGAAGGTGCGGCGCGAGGCCGAGCAGCGAGCGCGCGAAGAAAAGGA
  GCGCGAGCGCGAGCGGGAACGCGAGAAAGAGCGCGAGCGCGAGAAGGAGCGCGAGCTTGAACGCAGCGTG
  AAGTTGGCTCAGGAGGGCCGTGCTCCGGTGGAATGCCCATCTCTGGGCCCAGTGCCCCATCGCCCTCCAT
  TTGAACCGGGCAGTGCGGTGGCTACAGTGCCCCCCTACCTGGGTCCTGACACTCCAGCCTTGCGCACTCT
  CAGTGAATATGCCCGGCCTCATGTCATGTCTCCTGGCAATCGCAACCATCCATTCTACGTGCCCCTGGGG
  GCAGTGGACCCGGGGCTCCTGGGTTACAATGTCCCGGCCCTGTACAGCAGTGATCCAGCTGCCCGGGAGA
  GGGAACGGGAAGCCCGTGAACGAGACCTCCGTGACCGCCTCAAGCCTGGCTTTGAGGTGAAGCCTAGTGA
  GCTGGAACCCCTACATGGGGTCCCTGGGCCGGGCTTGGATCCCTTTCCCCGACATGGGGGCCTGGCTCTG
  CAGCCTGGCCCACCTGGCCTGCACCCTTTCCCCTTTCATCCGAGCCTGGGGCCCCTGGAGCGAGAACGTC
  TAGCGCTGGCAGCTGGGCCAGCCCTGCGGCCTGACATGTCCTATGCTGAGCGGCTGGCAGCTGAGAGGCA
  GCACGCAGAAAGGGTGGCGGCCCTGGGCAATGACCCACTGGCCCGGCTGCAGATGCTCAATGTGACTCCC
  CATCACCACCAGCACTCCCACATCCACTCGCACCTGCACCTGCACCAGCAAGATGCTATCCATGCAGCCT
  CTGCCTCGGTGCACCCTCTCATTGACCCCCTGGCCTCAGGGTCTCACCTTACCCGGATCCCCTACCCAGC
  TGGAACTCTCCCTAACCCCCTGCTTCCTCACCCTCTGCACGAGAACGAAGTTCTTCGTCACCAGCTCTTT
  GCTGCCCCTTACCGGGACCTGCCGGCCTCCCTTTCTGCCCCGATGTCAGCAGCTCATCAGCTGCAGGCCA
  TGCACGCACAGTCAGCTGAGCTGCAGCGCTTGGCGCTGGAACAGCAGCAGTGGCTGCATGCCCATCACCC
  GCTGCACAGTGTGCCGCTGCCTGCCCAGGAGGACTACTACAGTCACCTGAAGAAGGAAAGCGACAAGCCA
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215062 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKTRQNKDSMSMRSGRKKEAPGPREELRSRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVNKQ
  GRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGS
  VENDSDSSSGLSQGPARPYHPPPLFPPSPQPPDSTPRQPEASFEPHPSVTPTGYHAPMEPPTSRMFQAPP
  GAPPPHPQLYPGGTGGVLSGPPMGPKGGGAASSVGGPNGGKQHPPPTTPISVSSSGASGAPPTKPPTTPV
  GGGNLPSAPPPANFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQGMGGLPPGPEKGP
  TLAPSPHSLPPASSSAPAPPMRFPYSSSSSSSAAASSSSSSSSSSASPFPASQALPSYPHSFPPPTSLSV
  SNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSNAHPGPFPPSTGAQSTAHPPVSTHHHHHQQQQQQQ
  QQQQQQQQQQQQQHHGNSGPPPPGAFPHPLEGGSSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYS
  QAGPNGPPVSSSSNSSSSTSQGSYPCSHPSPSQGPQGAPYPFPPVPTVTTSSATLSTVIATVASSPAGYK
  TASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGTSPPAGPGTFKPGSPTVGPGPLPP
  AGPSGLPSLPPPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKH
  LDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSV
  KLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLG
  AVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLAL
  QPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTP
  HHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLF
  AAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKP
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007026
• ORF Size: 3573 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007026.1, NP_001007027.1
• RefSeq Size: 4367 bp
• RefSeq ORF: 3573 bp
• Locus ID: 1822
• UniProt ID: P54259
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome
• MW: 125.5 kDa
• Gene Summary: Dentatorubral pallidoluysian atrophy (DRPLA) is a rare neurodegenerative disorder characterized by cerebellar ataxia, myoclonic epilepsy, choreoathetosis, and dementia. The disorder is related to the expansion from 7-35 copies to 49-93 copies of a trinucleotide repeat (CAG/CAA) within this gene. The encoded protein includes a serine repeat and a region of alternating acidic and basic amino acids, as well as the variable glutamine repeat. Alternative splicing results in two transcripts variants that encode the same protein. [provided by RefSeq, Jul 2016]