IMPG1 (NM_001563) Human Tagged ORF Clone

CAT#: RC214997

IMPG1 (Myc-DDK-tagged)-Human interphotoreceptor matrix proteoglycan 1 (IMPG1)

Product Images

Circular map for RC214997

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  Western blot validation of overexpression lysate (Cat# LY419855) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214997 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IMPG1 protein (Cat# TP314997). The protein was produced from HEK293T cells transfected with IMPG1 cDNA clone (Cat# RC214997) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IMPG1
• Synonyms: GP147; IPM150; SPACR; VMD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214997 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTTGGAAACTAGAAGAGCTATTTTTGTTTTTTGGATTTTTCTCCAAGTTCAAGGAACCAAAGATA
  TCTCCATTAACATATACCATTCTGAAACTAAAGACATAGACAATCCCCCAAGAAATGAAACAACTGAAAG
  TACTGAAAAAATGTACAAAATGTCAACTATGAGACGAATATTCGATTTGGCAAAGCATCGAACAAAAAGA
  TCCGCATTTTTCCCAACGGGGGTTAAAGTCTGTCCACAGGAATCCATGAAACAGATTTTAGACAGTCTTC
  AAGCTTATTATAGATTGAGAGTGTGTCAGGAAGCAGTATGGGAAGCATATCGGATCTTTCTGGATCGCAT
  CCCTGACACAGGGGAATATCAGGACTGGGTCAGCATCTGCCAGCAGGAGACCTTCTGCCTCTTTGACATT
  GGAAAAAACTTCAGCAATTCCCAGGAGCACCTGGATCTTCTCCAGCAGAGAATAAAACAGAGAAGTTTCC
  CTGACAGAAAAGATGAAATATCTGCAGAGAAGACATTGGGAGAGCCTGGTGAAACCATTGTCATTTCAAC
  AGATGTTGCCAACGTCTCACTTGGGCCTTTCCCTCTCACTCCTGATGACACCCTCCTCAATGAAATTCTC
  GATAATACACTCAACGACACCAAGATGCCTACAACAGAAAGAGAAACAGAATTCGCTGTGTTGGAGGAGC
  AGAGGGTGGAGCTCAGCGTCTCTCTGGTAAACCAGAAGTTCAAGGCAGAGCTCGCTGACTCCCAGTCCCC
  ATATTACCAGGAGCTAGCAGGAAAGTCCCAACTTCAGATGCAAAAGATATTTAAGAAACTTCCAGGATTC
  AAAAAAATCCATGTGTTAGGATTTAGACCAAAGAAAGAAAAAGATGGCTCAAGCTCCACAGAGATGCAAC
  TTACGGCCATCTTTAAGAGACACAGTGCAGAAGCAAAAAGCCCTGCAAGTGACCTCCTGTCTTTTGATTC
  CAACAAAATTGAAAGTGAGGAAGTCTATCATGGAACCATGGAGGAGGACAAGCAACCAGAAATCTATCTC
  ACAGCTACAGACCTCAAAAGGCTGATCAGCAAAGCACTAGAGGAAGAACAATCTTTGGATGTGGGGACAA
  TTCAGTTCACTGATGAAATTGCTGGATCACTGCCAGCCTTTGGTCCTGACACCCAATCAGAGCTGCCCAC
  ATCTTTTGCTGTTATAACAGAGGATGCTACTTTGAGTCCAGAACTTCCTCCTGTTGAACCCCAGCTTGAG
  ACAGTGGACGGAGCAGAGCATGGTCTACCTGACACTTCTTGGTCTCCACCTGCTATGGCCTCTACCTCCC
  TGTCAGAAGCTCCACCTTTCTTTATGGCATCAAGCATCTTCTCTCTGACTGATCAAGGCACCACAGATAC
  AATGGCCACTGACCAGACAATGCTAGTACCAGGGCTCACCATCCCCACCAGTGATTATTCTGCAATCAGC
  CAACTGGCTCTGGGAATTTCACATCCACCTGCATCTTCAGATGACAGCCGATCAAGTGCAGGTGGCGAAG
  ATATGGTCAGAGACCTAGATGAAATGGATCTGTCTGACACTCCTGCCCCATCTGAGGTACCAGAGCTCAG
  CGAATATGTTTCTGTCCCAGATCATTTCTTGGAGGATACCACTCCTGTCTCAGCTTTACAGTATATCACC
  ACTAGTTCTATGACCATTGCCCCCAAGGGCCGAGAGCTGGTAGTGTTCTTCAGTCTGCGTGTTGCTAACA
  TGGCCTTCTCCAACGACCTGTTCAACAAGAGCTCTCTGGAGTACCGAGCTCTGGAGCAACAATTCACACA
  GCTGCTGGTTCCATATCTACGATCCAATCTTACAGGATTTAAGCAACTTGAAATACTTAACTTCAGAAAC
  GGGAGTGTGATTGTGAATAGCAAAATGAAGTTTGCTAAGTCAGTGCCGTATAACCTCACCAAGGCTGTGC
  ACGGGGTCTTGGAGGATTTTCGTTCTGCTGCAGCCCAACAACTCCATCTGGAAATAGACAGCTACTCTCT
  CAACATTGAACCAGCTGATCAAGCAGATCCCTGCAAGTTCCTGGCCTGCGGCGAATTTGCCCAATGTGTA
  AAGAACGAATGGACTGAGGAAGCGGAGTGTCGCTGCAAACCAGGATATGACAGCCAGGGGAGCCTGGACG
  GTCTGGAACCAGGCCTCTGTGGCCCTGGCACAAAGGAATGCGAGGTCCTCCAGGGAAAGGGAGCTCCATG
  CAGGTTGCCAGATCACTCTGAAAATCAAGCATACAAAACTAGTGTTAAAAAGTTCCAAAATCAACAAAAT
  AACAAGGTAATCAGTAAAAGAAATTCTGAATTACTGACCGTAGAATATGAAGAATTTAACCATCAAGATT
  GGGAAGGAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214997 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYLETRRAIFVFWIFLQVQGTKDISINIYHSETKDIDNPPRNETTESTEKMYKMSTMRRIFDLAKHRTKR
  SAFFPTGVKVCPQESMKQILDSLQAYYRLRVCQEAVWEAYRIFLDRIPDTGEYQDWVSICQQETFCLFDI
  GKNFSNSQEHLDLLQQRIKQRSFPDRKDEISAEKTLGEPGETIVISTDVANVSLGPFPLTPDDTLLNEIL
  DNTLNDTKMPTTERETEFAVLEEQRVELSVSLVNQKFKAELADSQSPYYQELAGKSQLQMQKIFKKLPGF
  KKIHVLGFRPKKEKDGSSSTEMQLTAIFKRHSAEAKSPASDLLSFDSNKIESEEVYHGTMEEDKQPEIYL
  TATDLKRLISKALEEEQSLDVGTIQFTDEIAGSLPAFGPDTQSELPTSFAVITEDATLSPELPPVEPQLE
  TVDGAEHGLPDTSWSPPAMASTSLSEAPPFFMASSIFSLTDQGTTDTMATDQTMLVPGLTIPTSDYSAIS
  QLALGISHPPASSDDSRSSAGGEDMVRDLDEMDLSDTPAPSEVPELSEYVSVPDHFLEDTTPVSALQYIT
  TSSMTIAPKGRELVVFFSLRVANMAFSNDLFNKSSLEYRALEQQFTQLLVPYLRSNLTGFKQLEILNFRN
  GSVIVNSKMKFAKSVPYNLTKAVHGVLEDFRSAAAQQLHLEIDSYSLNIEPADQADPCKFLACGEFAQCV
  KNEWTEEAECRCKPGYDSQGSLDGLEPGLCGPGTKECEVLQGKGAPCRLPDHSENQAYKTSVKKFQNQQN
  NKVISKRNSELLTVEYEEFNHQDWEGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001563
• ORF Size: 2391 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001563.4
• RefSeq Size: 3576 bp
• RefSeq ORF: 2394 bp
• Locus ID: 3617
• UniProt ID: Q17R60
• Cytogenetics: 6q14.1
• Protein Families: Druggable Genome, Secreted Protein
• MW: 89.4 kDa
• Gene Summary: This gene encodes a protein that is a major component of the retinal interphotoreceptor matrix. The encoded protein is a proteoglycan that is thought to play a role in maintaining viability of photoreceptor cells and in adhesion of the neural retina to the retinal pigment epithelium. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]