DBF4B (NM_145663) Human Tagged ORF Clone

CAT#: RC214891

DBF4B (Myc-DDK-tagged)-Human DBF4 homolog B (S. cerevisiae) (DBF4B), transcript variant 1

Product Images

Circular map for RC214891

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  Western blot validation of overexpression lysate (Cat# LY407888) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214891 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DBF4B protein (Cat# TP314891). The protein was produced from HEK293T cells transfected with DBF4B cDNA clone (Cat# RC214891) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBF4B
• Synonyms: ASKL1; CHIFB; DRF1; ZDBF1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214891 representing NM_145663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAACCGGGAAAGGGAGACGATTGCCTCGAGCTGGAGAGTTCCATGGCTGAGAGTAGGCTCCGGG
  CCCCGGACCTAGGAGTTTCCAGGTGTCTAGGAAAATGCCAGAAGAACTCACCAGGTGCCAGGAAGCATCC
  CTTTTCCGGAAAGTCCTTTTACTTGGATCTGCCTGCTGGCAAGAATCTCCAGTTTTTGACGGGGGCCATT
  CAGCAACTGGGTGGGGTAATTGAGGGTTTTCTGAGCAAAGAAGTAAGTTACATCGTGTCCAGCCGCAGAG
  AAGTAAAGGCAGAGAGCAGTGGGAAAAGCCATAGAGGCTGCCCTAGCCCTAGCCCCAGTGAGGTCAGAGT
  GGAAACATCGGCCATGGTTGATCCAAAAGGCAGCCACCCCAGGCCTTCACGGAAACCCGTTGACTCGGTG
  CCTCTAAGCAGAGGGAAGGAGCTGCTGCAGAAGGCTATCAGAAACCAGGGGAGCATCAGTGGAGGAGGCA
  GTGGGGGCAGCAGCAGCCTCCTGACCAATGCCCGCTCTTGGGGAGTGAGGATTCTGCACGTGGATGAAAT
  GATGATGCACGTGCAACAGCTGTCTCTTGCGTCTTTATGTGTGAAAAAACAACAGCCAAAGAAGCCAGAG
  GGAACATGTCCAGCAGCAGAGTCAAGAACACGGAAAGTGGCCAGACTGAAGGCCCCGTTCCTCAAAATCG
  AAGATGAAAGCAGGAAGTTTCGTCCTTTCCATCATCAGTTTAAATCCTTTCCTGAAATTTCTTTTCTTGG
  ACCCAAAGATGCAAGTCCCTTTGAGGCCCCGACGACCCTGGGCAGCATGCACCATACCAGAGAATCCAAG
  GATGGAGAGCCAAGCCCACGATCAGCTGCCCACACCATGCCCAGGAGGAAGAAAGGCTACTGCGAGTGCT
  GTCAGGAGGCCTTCGAGGAGCTCCATGTGCATCTTCAGAGTGCCCAGCACCGGAGCTTTGCCCTGGAAGC
  CCATCTATATGCAGAAGTGGACAGGATCATTGCTCAGCTCAGCCACAGCTTTGCAGACATCCCTTTCCAG
  GCTGGCCTCCCCAGGTGGTCAGGTTCCCCAGCTTCTGATTGTGACCCTCTCTGTCCTGAGACTCTGCACC
  CCCATCAGCCCTCCCATCCCAGGGCAGCATCTCCCAGGATAAGGAAAGAAGACAGCTGCCAGGCATCAGT
  GACCCAAGGCAGGGCTGCGGGCCAGCAGCGATGGACAGAATCACTAGATGGTGTGATGGGACCTCCTGCA
  AGTCACACATGTGTGAGTGCCACAACCCTCCTGCCGGCCTTGCCCAAGGGCTCCAGGGAGCAGGGCTGCC
  TCTGTCCCTGCCCAGCCTCCTTTACCCAGTCTCATCTGGTCACTTCCTTGGCTCTGCTGCCTGGGGAGTG
  GTCGCCTGCAGAGGACATGCCCCTCCATCCCTCCCAAGAAAACTCCTTTGCCCCGGCGGACATTCCTGTT
  AAGGGCCCACTCCTCTTCCCTGAAGCCAGACCGTGGCTTATGTCTGCACGCTGCTGGGTTCGTCCCTTTC
  CTTTTGTGACATGGGGTTGCCTCATTCCCCATGACACCACCCCTCTGCATGAGGAAGTTTCCCCTTGCCC
  CTGTCTCAGACTTGGATACCTTTACCTGCTGCTCACACAAAGCCTGTGGTGCCGGGTTCGGGTGCCCTCA
  TTGTCAACTGCAGGACCCATTCCCCGAACCTCACATCCGTGTACCCTTGCCTTCCCCTCCTATCTCAATG
  ATCATGACCTTGGACATCTCTGCCAGGCCAAACCCCAAGGCTGGAACACTCCTCAGCCATTTCTCCATTG
  CGGCTTCCTGGCTGTAGACTCAGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214891 representing NM_145663
  Red=Cloning site Green=Tags(s)
  
  MSEPGKGDDCLELESSMAESRLRAPDLGVSRCLGKCQKNSPGARKHPFSGKSFYLDLPAGKNLQFLTGAI
  QQLGGVIEGFLSKEVSYIVSSRREVKAESSGKSHRGCPSPSPSEVRVETSAMVDPKGSHPRPSRKPVDSV
  PLSRGKELLQKAIRNQGSISGGGSGGSSSLLTNARSWGVRILHVDEMMMHVQQLSLASLCVKKQQPKKPE
  GTCPAAESRTRKVARLKAPFLKIEDESRKFRPFHHQFKSFPEISFLGPKDASPFEAPTTLGSMHHTRESK
  DGEPSPRSAAHTMPRRKKGYCECCQEAFEELHVHLQSAQHRSFALEAHLYAEVDRIIAQLSHSFADIPFQ
  AGLPRWSGSPASDCDPLCPETLHPHQPSHPRAASPRIRKEDSCQASVTQGRAAGQQRWTESLDGVMGPPA
  SHTCVSATTLLPALPKGSREQGCLCPCPASFTQSHLVTSLALLPGEWSPAEDMPLHPSQENSFAPADIPV
  KGPLLFPEARPWLMSARCWVRPFPFVTWGCLIPHDTTPLHEEVSPCPCLRLGYLYLLLTQSLWCRVRVPS
  LSTAGPIPRTSHPCTLAFPSYLNDHDLGHLCQAKPQGWNTPQPFLHCGFLAVDSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145663
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145663.3
• RefSeq Size: 3081 bp
• RefSeq ORF: 1848 bp
• Locus ID: 80174
• UniProt ID: Q8NFT6
• Cytogenetics: 17q21
• MW: 67.1 kDa
• Gene Summary: This gene encodes a regulator of the cell division cycle 7 homolog (S. cerevisiae) protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and, in complex with the cell division cycle 7 homolog (S. cerevisiae) protein, may facilitate M phase progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]