OR52R1 (NM_001005177) Human Tagged ORF Clone

CAT#: RC214886

OR52R1 (Myc-DDK-tagged)-Human olfactory receptor, family 52, subfamily R, member 1 (OR52R1)

Product Images

Circular map for RC214886

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR52R1
• Synonyms: OR11-22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214886 representing NM_001005177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTACCCCATTTTGAATGACATAAGTACGAAAAACAACAGTAACATCATGTCATGTTGTAACATAT
  TATTTATTAAAACAGTTGAAATTATTCTAGTTTATAATCAAACCCAATCACCCTGGTATCCAATAGTCCC
  ATCCAAAAGCCTTGTATATAATAATAACACTTGTTTTGATTGTTATCATCTGCAGAGAGTAGATTGCGTT
  CCCAGCAGAGACCATATTAACCAGTCCATGGTGCTGGCTTCAGGGAACAGCTCTTCTCATCCTGTGTCCT
  TCATCCTGCTTGGAATCCCAGGCCTGGAGAGTTTCCAGTTGTGGATTGCCTTTCCGTTCTGTGCCACGTA
  TGCTGTGGCTGTTGTTGGAAATATCACTCTCCTCCATGTAATCAGAATTGACCACACCCTGCATGAGCCC
  ATGTACCTCTTTCTGGCCATGCTGGCCATCACTGACCTGGTCCTCTCCTCCTCCACTCAACCTAAGATGT
  TGGCCATATTCTGGTTTCATGCTCATGAGATTCAGTACCATGCCTGCCTCATCCAGGTGTTCTTCATCCA
  TGCCTTTTCTTCTGTGGAGTCTGGGGTGCTCATGGCTATGGCCCTGGACTGCTACGTGGCTACCTGCTTC
  CCACTCCGACACTCTAGCATCCTGACCCCATCGGTCGTGATCAAACTGGGGACCATCGTGATGCTGAGAG
  GGCTGCTGTGGGTGAGCCCCTTCTGCTTCATGGTGTCTAGGATGCCCTTCTGCCAACACCAAGCCATTCC
  CCAGTCATACTGTGAGCACATGGCTGTGCTGAAGTTGGTGTGTGCTGATACAAGCATAAGTCGTGGGTAT
  GGGCTCTTTGTGGCCTTCTCTGTGGCTGGCTTTGATATGATTGTCATTGGTATGTCATACGTGATGATTT
  TGAGAGCTGTGCTTCAGTTGCCCTCAGGTGAAGCCCGCCTCAAAGCTTTTAGCACACGTGCCTCCCATAT
  CTGTGTCATCTTGGCTCTTTATATCCCAGCCCTTTTTTCTTTCCTCACCTACCGCTTTGGCCATGATGTG
  CCCCGAGTTGTACACATCCTGTTTGCTAATCTCTATCTACTGATACCTCCCATGCTCAACCCCATCATTT
  ATGGAGTTAGAACCAAACAGATCGGGGACAGGGTTATCCAAGGATGTTGTGGAAACATCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214886 representing NM_001005177
  Red=Cloning site Green=Tags(s)
  
  MFYPILNDISTKNNSNIMSCCNILFIKTVEIILVYNQTQSPWYPIVPSKSLVYNNNTCFDCYHLQRVDCV
  PSRDHINQSMVLASGNSSSHPVSFILLGIPGLESFQLWIAFPFCATYAVAVVGNITLLHVIRIDHTLHEP
  MYLFLAMLAITDLVLSSSTQPKMLAIFWFHAHEIQYHACLIQVFFIHAFSSVESGVLMAMALDCYVATCF
  PLRHSSILTPSVVIKLGTIVMLRGLLWVSPFCFMVSRMPFCQHQAIPQSYCEHMAVLKLVCADTSISRGY
  GLFVAFSVAGFDMIVIGMSYVMILRAVLQLPSGEARLKAFSTRASHICVILALYIPALFSFLTYRFGHDV
  PRVVHILFANLYLLIPPMLNPIIYGVRTKQIGDRVIQGCCGNIP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005177
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005177.1, NP_001005177.1
• RefSeq Size: 948 bp
• RefSeq ORF: 948 bp
• Locus ID: 119695
• UniProt ID: Q8NGF1
• Cytogenetics: 11p15.4
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 44.2 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene is a segregating pseudogene, where some individuals have an allele that encodes a functional olfactory receptor, while other individuals have an allele encoding a protein that is predicted to be non-functional. [provided by RefSeq, Jun 2015]