PALD (PALD1) (NM_014431) Human Tagged ORF Clone

CAT#: RC214853

PALD1 (Myc-DDK-tagged)-Human KIAA1274 (KIAA1274)

Product Images

Circular map for RC214853

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  Western blot validation of overexpression lysate (Cat# LY415280) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214853 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PALD1 protein (Cat# TP314853). The protein was produced from HEK293T cells transfected with PALD1 cDNA clone (Cat# RC214853) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PALD
• Synonyms: KIAA1274; PALD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214853 representing NM_014431
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTACAACGGCCAGCACAGCCCAGCAGACGGTCTCGGCAGGCACCCCATTTGAGGGCCTACAGGGCA
  GTGGCACGATGGACAGTCGGCACTCCGTCAGCATCCACTCCTTCCAGAGCACTAGCTTGCATAACAGCAA
  GGCCAAGTCCATCATCCCCAACAAGGTGGCCCCTGTTGTGATCACGTACAACTGCAAGGAGGAGTTCCAG
  ATCCATGATGAGCTGCTCAAGGCTCATTACACGTTGGGCCGGCTCTCGGACAACACCCCTGAGCACTACC
  TGGTGCAAGGCCGCTACTTCCTGGTGCGGGATGTCACTGAGAAGATGGATGTGCTGGGCACCGTGGGAAG
  CTGTGGGGCCCCCAACTTCCGGCAGGTGCAGGGTGGGCTCACTGTGTTCGGCATGGGACAGCCCAGCCTC
  TCAGGGTTCAGGCGGGTCCTCCAGAAACTCCAGAAGGACGGACATAGGGAGTGTGTCATCTTCTGTGTGC
  GGGAGGAACCTGTGCTTTTCCTGCGTGCAGATGAGGACTTTGTGTCCTACACACCTCGAGACAAGCAGAA
  CCTTCATGAGAACCTCCAGGGCCTTGGACCCGGGGTCCGGGTGGAGAGCCTGGAGCTGGCCATCCGGAAA
  GAGATCCACGACTTTGCCCAGCTGAGCGAGAACACATACCATGTGTACCATAACACCGAGGACCTGTGGG
  GGGAGCCCCATGCTGTGGCCATCCATGGTGAGGACGACTTGCATGTGACGGAGGAGGTGTACAAGCGGCC
  CCTCTTCCTGCAGCCCACCTACAGGTACCACCGCCTGCCCCTGCCCGAGCAAGGGAGTCCCCTGGAGGCC
  CAGTTGGACGCCTTTGTCAGTGTTCTCCGGGAGACCCCCAGCCTGCTGCAGCTCCGTGATGCCCACGGGC
  CTCCCCCAGCCCTCGTCTTCAGCTGCCAGATGGGCGTGGGCAGGACCAACCTGGGCATGGTCCTGGGCAC
  CCTCATCCTGCTTCACCGCAGTGGGACCACCTCCCAGCCAGAGGCTGCCCCCACGCAGGCCAAGCCCCTG
  CCTATGGAGCAGTTCCAGGTGATCCAGAGCTTTCTCCGCATGGTGCCCCAGGGAAGGAGGATGGTGGAAG
  AGGTGGACAGAGCCATCACTGCCTGTGCCGAGTTGCATGACCTGAAAGAAGTGGTCTTGGAAAACCAGAA
  GAAGTTAGAAGGTATCCGACCGGAGAGCCCAGCCCAGGGAAGCGGCAGCCGACACAGCGTCTGGCAGAGG
  GCGCTGTGGAGCCTGGAGCGATACTTCTACCTGATCCTGTTTAACTACTACCTTCATGAGCAGTACCCGC
  TGGCCTTTGCCCTCAGTTTCAGCCGCTGGCTGTGTGCCCACCCTGAGCTGTACCGCCTGCCCGTGACGCT
  GAGCTCAGCAGGCCCTGTGGCTCCGAGGGACCTCATCGCCAGGGGCTCCCTACGGGAGGACGATCTGGTC
  TCCCCGGACGCGCTCAGCACTGTCAGAGAGATGGATGTGGCCAACTTCCGGCGGGTGCCCCGCATGCCCA
  TCTACGGCACGGCCCAGCCCAGCGCCAAGGCCCTGGGGAGCATCCTGGCCTACCTGACGGACGCCAAGAG
  GAGGCTGCGGAAGGTTGTCTGGGTGAGCCTTCGGGAGGAGGCCGTGTTGGAGTGTGACGGGCACACCTAC
  AGCCTGCGGTGGCCTGGGCCCCCTGTGGCTCCTGACCAGCTGGAGACCCTGGAGGCCCAGCTGAAGGCCC
  ATCTAAGCGAGCCTCCCCCAGGCAAGGAGGGCCCCCTGACCTACAGGTTCCAGACCTGCCTTACCATGCA
  GGAGGTCTTCAGCCAGCACCGCAGGGCCTGTCCTGGCCTCACCTACCACCGCATCCCCATGCCGGACTTC
  TGTGCCCCCCGAGAGGAGGACTTTGACCAGCTGCTGGAGGCCCTGCGGGCCGCCCTCTCCAAGGACCCAG
  GCACTGGCTTCGTGTTCAGCTGCCTCAGCGGCCAGGGCCGTACCACAACTGCGATGGTGGTGGCTGTCCT
  GGCCTTCTGGCACATCCAAGGCTTCCCCGAGGTGGGTGAGGAGGAGCTCGTGAGTGTGCCTGATGCCAAG
  TTCACTAAGGGTGAATTTCAGGTAGTAATGAAGGTGGTGCAGCTGCTACCCGATGGGCACCGTGTGAAGA
  AGGAGGTGGACGCAGCGCTGGACACTGTCAGCGAGACCATGACGCCCATGCACTACCACCTGCGGGAGAT
  CATCATCTGCACCTACCGCCAGGCGAAGGCAGCGAAAGAGGCGCAAGAAATGCGGAGGCTGCAGCTGCGG
  AGCCTGCAGTACTTGGAGCGCTATGTCTGCCTGATTCTCTTCAACGCGTACCTCCACCTGGAGAAGGCCG
  ACTCCTGGCAGAGGCCCTTCAGCACCTGGATGCAGGAGGTGGCATCGAAGGCTGGCATCTACGAGATCCT
  TAACGAGCTGGGCTTCCCCGAGCTGGAGAGCGGGGAGGACCAGCCCTTCTCCAGGCTGCGCTACCGGTGG
  CAGGAGCAGAGCTGCAGCCTCGAGCCCTCTGCCCCCGAGGACTTGCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214853 representing NM_014431
  Red=Cloning site Green=Tags(s)
  
  MGTTASTAQQTVSAGTPFEGLQGSGTMDSRHSVSIHSFQSTSLHNSKAKSIIPNKVAPVVITYNCKEEFQ
  IHDELLKAHYTLGRLSDNTPEHYLVQGRYFLVRDVTEKMDVLGTVGSCGAPNFRQVQGGLTVFGMGQPSL
  SGFRRVLQKLQKDGHRECVIFCVREEPVLFLRADEDFVSYTPRDKQNLHENLQGLGPGVRVESLELAIRK
  EIHDFAQLSENTYHVYHNTEDLWGEPHAVAIHGEDDLHVTEEVYKRPLFLQPTYRYHRLPLPEQGSPLEA
  QLDAFVSVLRETPSLLQLRDAHGPPPALVFSCQMGVGRTNLGMVLGTLILLHRSGTTSQPEAAPTQAKPL
  PMEQFQVIQSFLRMVPQGRRMVEEVDRAITACAELHDLKEVVLENQKKLEGIRPESPAQGSGSRHSVWQR
  ALWSLERYFYLILFNYYLHEQYPLAFALSFSRWLCAHPELYRLPVTLSSAGPVAPRDLIARGSLREDDLV
  SPDALSTVREMDVANFRRVPRMPIYGTAQPSAKALGSILAYLTDAKRRLRKVVWVSLREEAVLECDGHTY
  SLRWPGPPVAPDQLETLEAQLKAHLSEPPPGKEGPLTYRFQTCLTMQEVFSQHRRACPGLTYHRIPMPDF
  CAPREEDFDQLLEALRAALSKDPGTGFVFSCLSGQGRTTTAMVVAVLAFWHIQGFPEVGEEELVSVPDAK
  FTKGEFQVVMKVVQLLPDGHRVKKEVDAALDTVSETMTPMHYHLREIIICTYRQAKAAKEAQEMRRLQLR
  SLQYLERYVCLILFNAYLHLEKADSWQRPFSTWMQEVASKAGIYEILNELGFPELESGEDQPFSRLRYRW
  QEQSCSLEPSAPEDLL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014431
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014431.3
• RefSeq Size: 4569 bp
• RefSeq ORF: 2571 bp
• Locus ID: 27143
• UniProt ID: Q9ULE6
• Cytogenetics: 10q22.1
• MW: 96.6 kDa