PDE7A (NM_002603) Human Tagged ORF Clone

CAT#: RC214749

PDE7A (Myc-DDK-tagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 1

Product Images

Circular map for RC214749

• 

  Western blot validation of overexpression lysate (Cat# LY419221) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214749 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE7A
• Synonyms: HCP1; PDE7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214749 representing NM_002603
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATTACATTGATCTGGTGTCTGGCCTTGGTTCTTATCAAGTGGATCACCTCTAAGAGGCGTGGAG
  CTATTTCCTATGACAGTTCTGATCAGACTGCATTATACATTCGTATGCTAGGAGATGTACGTGTAAGGAG
  CCGAGCAGGATTTGAATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATTTTCCACTCTCAA
  TCTGAAATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAGCGATATCTTAGAT
  CTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGATGATGATTATAATGGACA
  AGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTATTTGATAGACTAACAAAT
  GGAAATAGTCTAGTAAGCTTAACCTTTCATTTATTTAGTCTTCATGGATTAATTGAGTACTTCCATTTAG
  ATATGATGAAACTTCGTAGATTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCTTACCATAA
  CGCAGTCCACGCTGCGGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTGCCAATTCT
  GTAACTCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGATCTGGATCATCCAGGTGTTA
  ATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAGAATACCTCAGTACTGGAAAA
  TCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGGCTTATTCTCACATCTGCCATTAGAAAGC
  AGGCAACAAATGGAGACACAGATAGGTGCTCTGATACTAGCCACAGACATCAGTCGCCAGAATGAGTATC
  TGTCTTTGTTTAGGTCCCATTTGGATAGAGGTGATTTATGCCTAGAAGACACCAGACACAGACATTTGGT
  TTTACAGATGGCTTTGAAATGTGCTGATATTTGTAACCCATGTCGGACGTGGGAATTAAGCAAGCAGTGG
  AGTGAAAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAAAAAAAATATCATTTGGGTGTGAGTC
  CACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATTGGTTTTATGACTTACCTAGTGGAGCC
  TTTATTTACAGAATGGGCCAGGTTTTCCAATACAAGGCTATCCCAGACAATGCTTGGACACGTGGGGCTG
  AATAAAGCCAGCTGGAAGGGACTGCAGAGAGAACAGTCGAGCAGTGAGGACACTGATGCTGCATTTGAGT
  TGAACTCACAGTTATTACCTCAGGAAAATCGGTTATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214749 representing NM_002603
  Red=Cloning site Green=Tags(s)
  
  MGITLIWCLALVLIKWITSKRRGAISYDSSDQTALYIRMLGDVRVRSRAGFESERRGSHPYIDFRIFHSQ
  SEIEVSVSARNIRRLLSFQRYLRSSRFFRGTAVSNSLNILDDDYNGQAKCMLEKVGNWNFDIFLFDRLTN
  GNSLVSLTFHLFSLHGLIEYFHLDMMKLRRFLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLANS
  VTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSVLENHHWRSAVGLLRESGLFSHLPLES
  RQQMETQIGALILATDISRQNEYLSLFRSHLDRGDLCLEDTRHRHLVLQMALKCADICNPCRTWELSKQW
  SEKVTEEFFHQGDIEKKYHLGVSPLCDRHTESIANIQIGFMTYLVEPLFTEWARFSNTRLSQTMLGHVGL
  NKASWKGLQREQSSSEDTDAAFELNSQLLPQENRLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002603
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002603.4
• RefSeq Size: 1739 bp
• RefSeq ORF: 1371 bp
• Locus ID: 5150
• UniProt ID: Q13946
• Cytogenetics: 8q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 52.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]