IKB epsilon (NFKBIE) (NM_004556) Human Tagged ORF Clone

CAT#: RC214683

NFKBIE (Myc-DDK-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon (NFKBIE)

Product Images

Circular map for RC214683

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NFKBIE (Cat# RC214683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIE (Cat# TA810713)(1:2000).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IKB epsilon
• Synonyms: IKBE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214683 representing NM_004556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCAACGAAGGAGTGAGTCAAGGCCCGGGAACCACAGACTCCAAGCCTACGCAGAGCCCGGGAAGG
  GGGATTCCGGAGGGGCGGGGCCTCTTTCCGGAAGCGCCCGCCGGGGGCGGGGAGGGGGCGGGGCCATCCG
  CGTGAGGCGACCCTGTTGGTCCGGAGGGGCGGGGCGAGGAGGAGGACCCGCTTGGGCGGTTCGGCTGCCC
  ACAGTAACCGCTGGGTGGACCTGGCCAGCGCTCCGAACCTTGTCCTCGCTGCGCGCCGGCCCCTCGGAGC
  CCCACAGCCCGGGAAGGAGGCCGCCGCGGGCCGGGCGCCCGCTCTGCCAAGCGGACCCGCAACCCGGAAA
  GGCGGCGCGGCGGAGCCTGGAGCCGGATCCTGCTCAGACCGGGCCCCGGCCGGCCAGAGCCGCGGGCATG
  TCGGAGGCGCGGAAGGGGCCGGACGAGGCGGAGGAGAGCCAGTACGACTCTGGCATTGAGTCTCTGCGCT
  CTCTGCGCTCCCTACCCGAGTCCACCTCGGCTCCAGCCTCCGGGCCCTCGGACGGCAGCCCCCAGCCCTG
  CACCCATCCTCCGGGACCCGTCAAGGAACCACAGGAGAAGGAAGACGCGGATGGGGAGCGGGCTGATTCC
  ACCTATGGCTCCTCCTCGCTCACCTACACCCTGTCCTTGCTGGGGGGCCCCGAGGCTGAGGACCCGGCCC
  CACGCCTGCCACTCCCCCACGTGGGGGCGCTGAGCCCTCAGCAGCTGGAAGCACTCACTTACATCTCCGA
  GGACGGAGACACGCTGGTCCACCTGGCAGTGATTCATGAGGCCCCAGCGGTGCTGCTCTGTTGCCTGGCT
  TTGCTGCCCCAGGAGGTCCTGGACATTCAAAATAACCTTTACCAGACAGCACTCCATCTGGCTGTACATC
  TGGACCAACCGGGCGCAGTTCGGGCACTGGTGCTGAAGGGGGCCAGCCGGGCACTACAGGACCGGCATGG
  TGACACAGCCCTTCATGTGGCCTGCCAGCGCCAGCACTTGGCCTGTGCCCGCTGCCTGCTGGAAGGGCGG
  CCAGAGCCAGGCAGAGGAACATCTCACTCTCTGGACCTCCAGCTGCAAAACTGGCAAGGTCTGGCTTGTC
  TCCACATTGCCACCCTTCAGAAGAACCAACCACTCATGGAATTGCTGCTTCGGAATGGAGCTGACATTGA
  TGTGCAGGAGGGCACCAGTGGTAAGACAGCGCTGCACCTGGCTGTGGAAACCCAAGAGCGGGGCCTGGTA
  CAGTTCCTGCTCCAGGCTGGTGCCCAGGTAGATGCCCGCATGCTGAACGGGTGCACACCCCTGCACCTGG
  CAGCTGGCCGGGGTCTCATGGGCATCTCATCCACTCTGTGCAAGGCGGGTGCTGACTCCCTGCTGCGGAA
  TGTGGAGGATGAGACGCCCCAGGACCTGACTGAGGAATCCCTTGTCCTTTTGCCCTTTGATGACCTGAAG
  ATCTCAGGGAAACTGCTGCTGTGTACCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214683 representing NM_004556
  Red=Cloning site Green=Tags(s)
  
  MNQRRSESRPGNHRLQAYAEPGKGDSGGAGPLSGSARRGRGGGGAIRVRRPCWSGGAGRGGGPAWAVRLP
  TVTAGWTWPALRTLSSLRAGPSEPHSPGRRPPRAGRPLCQADPQPGKAARRSLEPDPAQTGPRPARAAGM
  SEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADS
  TYGSSSLTYTLSLLGGPEAEDPAPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLA
  LLPQEVLDIQNNLYQTALHLAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQHLACARCLLEGR
  PEPGRGTSHSLDLQLQNWQGLACLHIATLQKNQPLMELLLRNGADIDVQEGTSGKTALHLAVETQERGLV
  QFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKAGADSLLRNVEDETPQDLTEESLVLLPFDDLK
  ISGKLLLCTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004556
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004556.2, NP_004547.2
• RefSeq Size: 2581 bp
• RefSeq ORF: 1086 bp
• Locus ID: 4794
• UniProt ID: O00221
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, B cell receptor signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway
• MW: 52.7 kDa
• Gene Summary: The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]