ANKLE1 (NM_152363) Human Tagged ORF Clone

CAT#: RC214643

ANKLE1 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 1 (ANKLE1)

Product Images

Circular map for RC214643

• 

  Western blot validation of overexpression lysate (Cat# LY407613) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214643 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKLE1
• Synonyms: ANKRD41; LEM3; LEMD6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214643 representing NM_152363
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCGGAGGCCCGCCTGGCTCGCAGGTTGCGGGATGCGCTGCGGGAGGAGGAGCCGTGGGCAGTAG
  AGGAGCTGCTGCGCTGCGGCGCGGACCCTAATTTGGTGCTAGAGGACGGCGCAGCGGCTGTGCACTTGGC
  GGCCGGAGCCCGGCACCCGCGCGGCCTGCGTTGCCTCGGGGCCCTACTGCGCCAAGGCGGGGACCCCAAC
  GCTCGATCTGTCGAGGCACTGACGCCGCTGCATGTGGCCGCCGCGTGGGGCTGCCGCCGCGGCCTGGAGC
  TGCTGCTGAGCCAAGGAGCGGACCCGGCGCTGCGCGACCAGGACGGACTCCGGCCGCTGGACCTGGCCCT
  GCAGCAGGGACACCTGGAGTGCGCGCGAGTCCTGCAGGATCTCGACACGCGGACCAGGACCCGGACCCGG
  ATCGGGGCAGAGACTCAGGAGCCCGAGCCTGCACCTGGCACCCCAGGCCTCTCTGGACCTACCGATGAGA
  CGCTGGACTCCATAGCACTCCAAAAGCAGCCATGCAGAGGTGACAACAGGGACATTGGCTTGGAGGCTGA
  CCCAGGACCCCCCAGCCTCCCTGTTCCCCTTGAAACTGTGGACAAACATGGGAGCTCGGCGTCCCCTCCA
  GGGCACTGGGATTACAGCTCAGACGCCTCTTTCGTCACAGCGGTTGAGGTCTCTGGAGCTGAGGACCCAG
  CCTCGGACACTCCCCCCTGGGCTGGGTCATTGCCACCGACCAGGCAGGGACTTCTGCATGTTGTCCATGC
  CAACCAGAGGGTACCTAGGTCTCAGGGCACGGAGGCAGAACTGAATGCCCGTCTGCAGGCCCTGACTCTG
  ACCCCACCAAATGCTGCTGGCTTCCAGTCCTCCCCTTCCTCCATGCCTCTCCTGGACAGGAGTCCAGCTC
  ATAGCCCCCCACGGACACCAACCCCTGGAGCTTCTGACTGCCACTGCCTGTGGGAGCACCAGACATCCAT
  TGATAGTGACATGGCCACGCTCTGGCTGACAGAGGATGAGGCAAGCTCTACAGGTGGCAGGGAACCTGTC
  GGCCCTTGCCGGCACCTGCCAGTCTCCACTGTGTCTGACTTGGAGTTGCTGAAGGGACTCCGAGCACTTG
  GTGAGAATCCTCACCCCATCACACCCTTCACCAGGCAGTTGTACCACCAGCAGCTGGAAGAAGCCCAGAT
  TGCTCCTGGCCCAGAGTTTTCAGGGCACAGCCTAGAACTGGCTGCAGCCCTGCGGACGGGCTGTATTCCA
  GATGTCCAGGCAGATGAAGACGCGCTGGCCCAGCAGTTTGAGCAGCCAGATCCTGCCAGGAGGTGGCGGG
  AGGGGGTCGTGAAGTCTAGCTTCACCTATCTGCTGCTGGACCCCAGGGAGACTCAGGACCTGCCAGCCCG
  AGCCTTCTCACTGACCCCAGCTGAGCGCCTTCAGACTTTCATCCGTGCCATCTTCTACGTGGGCAAAGGG
  ACGAGGGCCCGGCCATATGTCCACCTCTGGGAGGCCCTTGGTCACCATGGGCGGTCAAGAAAACAGCCCC
  ACCAGGCCTGCCCCAAGGTGCGTCAGATCTTGGACATCTGGGCCAGTGGTTGCGGTGTTGTGTCCCTACA
  TTGCTTCCAGCACGTGGTCGCTGTGGAGGCTTATACACGGGAGGCGTGTATTGTGGAAGCCCTAGGGATC
  CAGACGCTCACCAACCAGAAGCAAGGGCACTGCTATGGAGTGGTGGCAGGCTGGCCACCTGCTCGTCGCC
  GGCGCTTGGGGGTGCACCTGCTGCACCGTGCCCTCCTTGTCTTCCTGGCTGAAGGCGAGCGACAGCTTCA
  TCCCCAGGACATCCAGGCCCGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214643 representing NM_152363
  Red=Cloning site Green=Tags(s)
  
  MCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPN
  ARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTR
  IGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPP
  GHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTL
  TPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPV
  GPCRHLPVSTVSDLELLKGLRALGENPHPITPFTRQLYHQQLEEAQIAPGPEFSGHSLELAAALRTGCIP
  DVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKG
  TRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGI
  QTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152363
• ORF Size: 1845 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152363.6
• RefSeq Size: 3217 bp
• RefSeq ORF: 1848 bp
• Locus ID: 126549
• UniProt ID: Q8NAG6
• Cytogenetics: 19p13.11
• MW: 66.7 kDa
• Gene Summary: Endonuclease that probably plays a role in the DNA damage response and DNA repair.[UniProtKB/Swiss-Prot Function]