CNG1 (CNGA1) (NM_000087) Human Tagged ORF Clone

CAT#: RC214525

CNGA1 (Myc-DDK-tagged)-Human cyclic nucleotide gated channel alpha 1 (CNGA1), transcript variant 2

Product Images

Circular map for RC214525

• 

  Western blot validation of overexpression lysate (Cat# LY400028) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214525 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNG1
• Synonyms: CNCG; CNCG1; CNG-1; CNG1; RCNC1; RCNCa; RCNCalpha; RP49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214525 representing NM_000087
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTATCCATGAAGAACAATATTATCAATACACAGCAGTCTTTTGTAACCATGCCCAATGTGATTG
  TACCAGATATTGAAAAGGAAATACGAAGGATGGAAAATGGAGCATGCAGCTCCTTTTCTGAGGATGATGA
  CAGTGCCTCTACATCTGAAGAATCAGAGAATGAAAACCCTCATGCAAGGGGTTCCTTTAGTTATAAGTCA
  CTCAGAAAGGGAGGACCATCACAGAGGGAGCAGTACCTGCCTGGTGCCATTGCACTTTTTAATGTGAACA
  ACAGCAGCAATAAGGACCAGGAACCAGAAGAAAAAAAGAAAAAGAAAAAAGAAAAGAAGAGCAAGTCAGA
  TGATAAAAACGAAAATAAAAACGACCCAGAGAAGAAAAAGAAGAAAAAGGACAAAGAGAAGAAAAAGAAA
  GAGGAGAAAAGCAAAGATAAGAAAGAAGAGGAGAAGAAAGAAGTTGTGGTTATTGATCCCTCGGGAAACA
  CATATTACAACTGGCTGTTTTGCATCACATTACCTGTTATGTACAACTGGACAATGGTTATTGCCAGAGC
  ATGTTTTGATGAACTTCAATCTGATTACCTAGAATATTGGCTCATTTTGGATTACGTATCAGACATAGTC
  TATTTAATCGATATGTTTGTACGAACAAGGACAGGTTACCTAGAACAAGGACTGCTGGTAAAGGAAGAAC
  TTAAACTCATAAATAAATATAAATCCAACTTGCAATTTAAACTTGATGTTCTGTCACTGATACCAACTGA
  TTTGCTGTATTTTAAGTTAGGGTGGAACTATCCAGAAATTAGATTAAACAGGTTGTTACGGTTCTCTCGT
  ATGTTTGAGTTCTTCCAGAGAACAGAAACAAGGACAAACTATCCAAACATCTTCAGGATTTCCAACCTTG
  TTATGTATATCGTCATCATTATCCACTGGAATGCATGTGTGTTCTACTCTATTTCTAAAGCTATTGGATT
  TGGAAATGATACATGGGTCTACCCTGATATTAATGATCCTGAATTTGGCCGTTTGGCTAGAAAATACGTA
  TACAGCCTTTACTGGTCTACACTGACTTTGACTACCATTGGTGAAACACCCCCTCCCGTGAGGGATTCTG
  AGTATGTCTTTGTGGTGGTTGATTTCCTAATTGGAGTGTTAATTTTTGCTACCATCGTTGGTAACATAGG
  TTCTATGATTTCCAACATGAATGCAGCCAGAGCAGAATTTCAAGCAAGAATTGATGCTATCAAGCAATAT
  ATGCATTTTCGAAATGTAAGCAAAGATATGGAAAAGAGGGTTATTAAATGGTTTGACTACCTGTGGACCA
  ACAAAAAAACAGTTGATGAGAAAGAAGTCTTAAAGTATCTACCTGATAAACTAAGAGCAGAAATTGCCAT
  CAACGTTCACTTAGACACATTAAAAAAGGTACGCATTTTTGCTGATTGTGAAGCTGGTCTGTTGGTGGAG
  TTGGTCTTGAAATTGCAACCCCAAGTCTACAGTCCTGGAGATTATATTTGCAAGAAAGGGGATATCGGAC
  GAGAGATGTACATTATCAAGGAAGGCAAACTCGCTGTGGTGGCAGATGATGGAGTCACTCAGTTTGTGGT
  ATTGAGCGATGGCAGCTACTTCGGTGAGATCAGCATTCTTAACATTAAAGGGAGCAAAGCTGGCAATCGA
  AGAACGGCCAATATTAAAAGTATTGGCTACTCAGACCTGTTCTGTCTCTCAAAAGATGACCTCATGGAAG
  CTCTAACTGAGTACCCAGATGCCAAAACTATGCTGGAAGAGAAAGGGAAGCAGATTTTAATGAAAGATGG
  TCTACTGGATCTAAACATTGCAAATGCTGGCAGTGATCCTAAAGATCTTGAAGAGAAGGTTACTCGAATG
  GAGGGGTCAGTAGACCTCCTGCAAACCAGGTTTGCCCGAATCTTGGCTGAGTATGAGTCCATGCAGCAGA
  AACTGAAACAAAGATTAACCAAGGTTGAGAAATTTCTGAAACCGCTTATTGACACAGAATTTTCAAGTAT
  TGAGGGACCTGGAGCGGAAAGTGGGCCCATCGACTCTACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214525 representing NM_000087
  Red=Cloning site Green=Tags(s)
  
  MKLSMKNNIINTQQSFVTMPNVIVPDIEKEIRRMENGACSSFSEDDDSASTSEESENENPHARGSFSYKS
  LRKGGPSQREQYLPGAIALFNVNNSSNKDQEPEEKKKKKKEKKSKSDDKNENKNDPEKKKKKKDKEKKKK
  EEKSKDKKEEEKKEVVVIDPSGNTYYNWLFCITLPVMYNWTMVIARACFDELQSDYLEYWLILDYVSDIV
  YLIDMFVRTRTGYLEQGLLVKEELKLINKYKSNLQFKLDVLSLIPTDLLYFKLGWNYPEIRLNRLLRFSR
  MFEFFQRTETRTNYPNIFRISNLVMYIVIIIHWNACVFYSISKAIGFGNDTWVYPDINDPEFGRLARKYV
  YSLYWSTLTLTTIGETPPPVRDSEYVFVVVDFLIGVLIFATIVGNIGSMISNMNAARAEFQARIDAIKQY
  MHFRNVSKDMEKRVIKWFDYLWTNKKTVDEKEVLKYLPDKLRAEIAINVHLDTLKKVRIFADCEAGLLVE
  LVLKLQPQVYSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKAGNR
  RTANIKSIGYSDLFCLSKDDLMEALTEYPDAKTMLEEKGKQILMKDGLLDLNIANAGSDPKDLEEKVTRM
  EGSVDLLQTRFARILAEYESMQQKLKQRLTKVEKFLKPLIDTEFSSIEGPGAESGPIDST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000087
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000087.4
• RefSeq Size: 2532 bp
• RefSeq ORF: 2061 bp
• Locus ID: 1259
• UniProt ID: P29973
• Cytogenetics: 4p12
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• MW: 79.4 kDa
• Gene Summary: The protein encoded by this gene is involved in phototransduction. Along with another protein, the encoded protein forms a cGMP-gated cation channel in the plasma membrane, allowing depolarization of rod photoreceptors. This represents the last step in the phototransduction pathway. Defects in this gene are a cause of retinitis pigmentosa autosomal recessive (ARRP) disease. Multiple transcript variants have been found for this gene. [provided by RefSeq, Oct 2019]