CCDC150 (NM_001080539) Human Tagged ORF Clone

CAT#: RC214293

CCDC150 (Myc-DDK-tagged)-Human coiled-coil domain containing 150 (CCDC150)

Product Images

Circular map for RC214293

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214293 representing NM_001080539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGTAAGGTACATATGGAAACTACAGTGTCCAGACCGGTCCTTTCTCCAACCCACATCAACGCTA
  CAGCTTCTGAAACATTCACAGTACTTCAGCAAAGGATGAGAATAGTTGAGGAACAGACCAGTTCACTGAG
  GGATGACCTAATAATGTTGGATTTTGGTGAAAAAAGAGGCTATTTGGAAGCTCCAGACTGTTTAGAAGAC
  CTGGACAGCCAGAAAGTCATTAGTCCTATCCAAAATGAAGCAATTTGTGCAGGAAAAACAGATATTTTAT
  GGAAGAACTGTGAGTTTCTGGTAAATCGAATGTGCCGTCTTGAAAGCCTCATGCAGTCCTTGAAGATGAA
  CATCTTTCGGCTGCAAACTGAAAAGGATTTGAATCCTCAGAAAACAGCTTTTCTGAAAGATCGACTGAAT
  GCAATACAGGAAGAGCATTCTAAGGACCTGAAGCTGTTGCATCTCGAAGTTATGAATTTGCGCCAGCAAC
  TGAGAGCTGTAAAAGAGGAAGAAGACAAGGCACAAGATGAGGTGCAAAGGTTGACTGCCACTCTGAAGAT
  TGCCTCGCAGACAAAGAAGAATGCAGCCATTATTGAAGAGGAACTGAAGACCACAAAACGTAAAATGAAC
  CTTAAAATTCAAGAGCTAAGGAGACAACTGGCTCAGGAGAAGTACCTTAGGGAATCTTTAGAGAAATCAG
  CATCAGCCATGCTCCTCAAAATACAAGAAATGGGATCAACAGTGGAGGTAGAACGAAAACAGGTGCACAT
  TTTGCAGCAAAACTGCATTGCTCTACGTGATTCTATACAGAGCGCTCAAGAACTACTGGCCCAGGAACAA
  AAAAAAAAAGAAGAGTTGGAGATTGCTACTTCACAGCTCAAATCTGATCTAACTTCTAGAGATGACCTCA
  TTTCCAAGTTGGTTGAAGAAAATAAGAACCTGCAGATATCTTTCAACAAGGAACATGAAGAAAATGCATA
  TTTGAGGTCCGAAATAATGTCTCTTCATGAAGCATCAGAAAAAGCACAAGTTTTGAATGACCAATTGACT
  AAAAAGTGTTCAGAGTTGAGCTGCATGCTTCAGACTGTTACTATGGAAAAAGCCAGAATCATTGCTGACC
  ATCAGGCCATTCTGCAGGTAGAGCAGAAAATGATGACGCAGACATTTCAAGAACAAAACTTATTGCTGGA
  TGCAGCCCATGCCAGTATCACAAATGAACTACAGACTGTTCAGAATGAGAAAACCCAACTCCAGGCACAT
  CTGGATCATTTAATCCTTGAGCATAACCAGTGTATCCAGAAAGCACAGGATGCTGAAAAGAGAACAGCTG
  TGCAAAAAGAGCTGCTAGAATCAACTATTGCAAGATTGCGAGGTGAATTGGAAGCATCAATGCAAGAGAA
  GAAGTCTCTGCTAGAGGAGAAAGAAAGATTTCAGAGGGAGGTTAATAAAACAGAAAAAGAAATAGTGCAA
  GAAAGATGCAATTTGGAAAAGGAATTAGCTAAAAACAAGGTAGACATAAATACATTAACTCATAACCTGC
  AGACTCTTGAAGAAGAGAATAAGCACCTGGCAGATCAAATGGCTTCCCTAGAACTTCAGCAAGTCACTTC
  TGATTACCATGGGCTTGCCCAACAGAAGGTGGAAAAAATCACTGAAAGTAAAAATAAACTGGCCTATGAA
  AACGGAAAACTCCAGATAAAAGTTAAACAGCTAGAAGAACAAGTACAGTCTTTTACTGACACCAGCTTAC
  AGAATGATCATCTACGCAAGATGAATAAGTATTTACAGACTAAATATGCTCAGGCAAACTCAGAACTCAG
  TGCCAAGAGGGTACACCTGCAGCAGGCAGATGCTCACCTGAAAGAAGTGAAATCTATTCTTGAAAGAAGT
  AAAGAGGAGCTGTCCCGAACAGTGAAGTGTCGTAATGCGGCCCTGAAAGAGAGTCAGAAGTTGAAAGAAG
  ACCTCGAGGCTGTGGAGGACAGGGAAAACAAGAAGGTGGGAAACTTTCAGCGACAATTGGCAGAAGCTAA
  AGAAGACAACTGCAAAGTCACAATCATGTTGGAGAATGTGCTGGCTTCTCACAGTAAGATGCAAGGTGCT
  CTGGAGAAAGTACAAATAGAGCTTGGGCGGAGGGATTCAGAGATTGCAGGCCTCAAGAAAGAAAGGGATC
  TCAATCAACAGAGGGTGCAGAAGCTGGAAGCTGAAGTGGACCAGTGGCAGGCCAGGATGCTTGTCATGGA
  GGACCAGCACAACAGTGAGATTGAATCTCTACAAAAAGCTCTAGGTGTAGCTAGAGAAGACAACAGGAAA
  CTTGCTATGAGTCTGGAACAAGCTCTCCAGACAAATAATCATCTGCAAACAAAGCTAGATCACATTCAAG
  AGCAATTGGAAAGCAAAGAACTTGAGCGACAGAATTTGGAAACCTTCAAAGACCGGATGACTGAAGAGTC
  CAAAGTGGAAGCAGAATTGCATGCTGAACGCATAGAAGCTCTAAGAAAGCAGTTTCAAACCGAGAGAGAA
  ACTACAAAGAAAGTGGCACAACGGGAAGTGGCTGAGCTGAAGAAAGCCCTTGATGAAGCTAACTTCAGAT
  CAGTGGAAGTGTCCCGGACCAACCGAGAGCTGCGACAGAAACTTGCAGAGCTAGAAAAAATACTAGAAAG
  TAACAAGGAGAAAATAAAGAATCAAAAGACCCAAATTAAGCTCCACTTGTCAGCTAAGGCGAATAATGCT
  CAGAATATAGAAAGGATGAAGCAAATAGAAAAAGAATTGAAGCAAATGGAGCTAATTAAGGATCAATATC
  AGAAAAAGAACTATGAACAGTCTTTGAGTATCCAGAGATTTGTGTGTGAAATGACTAACCTGCAGAAAGA
  GATGCAGATGTTGGCTAAGAGCCAATATGATGCCTCAGTGCGGAATAAACAGCAAGAGCTGCACCTAGAA
  GCAGAGCGGAAAATAAGGCAGGAGCTAGAGAATCGGTGCCAGGAATTGGAAGAAACTGTCAGACACCTGA
  AGAAATGTAAAGAGGCAACAGAGAATACGCTGAAAGAAGCCAGTGTGGAATCAGAACAGATAACAGCTAA
  TCTGGAAGAAGCTCATCGCTGGTTTAAGCACAGGTTTGATGGTCTACAACTTGAGCTGACAAAAAACCGG
  TTGCAGAGGCCTTCTGGGGAAGACAGGTGGCAGGAAAAGGACCAAGATGTAAAACATGATGTCATGTCCA
  ACCAATCTGTTCTGCATCGATGGGAGAGAAAACAGAATCTTAGGCCCATGCCCAAGAAGTATCATTCTGA
  GGTACAGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214293 representing NM_001080539
  Red=Cloning site Green=Tags(s)
  
  MDCKVHMETTVSRPVLSPTHINATASETFTVLQQRMRIVEEQTSSLRDDLIMLDFGEKRGYLEAPDCLED
  LDSQKVISPIQNEAICAGKTDILWKNCEFLVNRMCRLESLMQSLKMNIFRLQTEKDLNPQKTAFLKDRLN
  AIQEEHSKDLKLLHLEVMNLRQQLRAVKEEEDKAQDEVQRLTATLKIASQTKKNAAIIEEELKTTKRKMN
  LKIQELRRQLAQEKYLRESLEKSASAMLLKIQEMGSTVEVERKQVHILQQNCIALRDSIQSAQELLAQEQ
  KKKEELEIATSQLKSDLTSRDDLISKLVEENKNLQISFNKEHEENAYLRSEIMSLHEASEKAQVLNDQLT
  KKCSELSCMLQTVTMEKARIIADHQAILQVEQKMMTQTFQEQNLLLDAAHASITNELQTVQNEKTQLQAH
  LDHLILEHNQCIQKAQDAEKRTAVQKELLESTIARLRGELEASMQEKKSLLEEKERFQREVNKTEKEIVQ
  ERCNLEKELAKNKVDINTLTHNLQTLEEENKHLADQMASLELQQVTSDYHGLAQQKVEKITESKNKLAYE
  NGKLQIKVKQLEEQVQSFTDTSLQNDHLRKMNKYLQTKYAQANSELSAKRVHLQQADAHLKEVKSILERS
  KEELSRTVKCRNAALKESQKLKEDLEAVEDRENKKVGNFQRQLAEAKEDNCKVTIMLENVLASHSKMQGA
  LEKVQIELGRRDSEIAGLKKERDLNQQRVQKLEAEVDQWQARMLVMEDQHNSEIESLQKALGVAREDNRK
  LAMSLEQALQTNNHLQTKLDHIQEQLESKELERQNLETFKDRMTEESKVEAELHAERIEALRKQFQTERE
  TTKKVAQREVAELKKALDEANFRSVEVSRTNRELRQKLAELEKILESNKEKIKNQKTQIKLHLSAKANNA
  QNIERMKQIEKELKQMELIKDQYQKKNYEQSLSIQRFVCEMTNLQKEMQMLAKSQYDASVRNKQQELHLE
  AERKIRQELENRCQELEETVRHLKKCKEATENTLKEASVESEQITANLEEAHRWFKHRFDGLQLELTKNR
  LQRPSGEDRWQEKDQDVKHDVMSNQSVLHRWERKQNLRPMPKKYHSEVQRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080539
• ORF Size: 3303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080539.2
• RefSeq Size: 3685 bp
• RefSeq ORF: 3306 bp
• Locus ID: 284992
• UniProt ID: Q8NCX0
• Cytogenetics: 2q33.1
• Protein Families: Protease
• MW: 128.6 kDa